FastQCFastQC Report
Thu 9 Feb 2017
SRR2727334.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727334.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4973386
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT364870.7336450458500506No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT141670.2848562327557121No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT132200.26581487944028476No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87620.17617775897547466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC52650.017.2877537
AACGCAG157650.015.5721535
CGCGAAC1353.979585E-715.07407514
TACGGAT9550.014.33507927
TATACTG8650.014.3294795
TTAGACT5700.014.2807024
ACGCAGA174850.014.1461256
TCGTTAC6950.014.10791523
CGCAGAG178600.013.8283877
GTCTTAT5550.013.6666671
AGAGTAC180400.013.628880510
GTTACGG8450.013.57396525
CTCGTTA7750.013.36774322
CAGAGTA186000.013.3379049
GTCTTAG9600.013.2968741
CAATACT7550.013.2317874
GCAGAGT188900.013.19198
TTACGGA10150.013.12315326
TAGACAG9450.013.1164035
GTACTAC5550.013.01
Produced by FastQC (version 0.11.5)