##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727334.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4973386
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.336047915846464	33.0	31.0	34.0	30.0	34.0
2	32.47944056624601	34.0	31.0	34.0	31.0	34.0
3	32.573557129890986	34.0	31.0	34.0	31.0	34.0
4	36.107302952153724	37.0	35.0	37.0	35.0	37.0
5	35.915195603156484	37.0	35.0	37.0	35.0	37.0
6	35.93243898623594	37.0	35.0	37.0	35.0	37.0
7	35.89872312344146	37.0	35.0	37.0	35.0	37.0
8	35.85449108514803	37.0	35.0	37.0	35.0	37.0
9	37.69134509165386	39.0	37.0	39.0	35.0	39.0
10	37.51879584653192	39.0	37.0	39.0	35.0	39.0
11	37.62625704097772	39.0	37.0	39.0	35.0	39.0
12	37.54853574606918	39.0	37.0	39.0	35.0	39.0
13	37.62801881856747	39.0	37.0	39.0	35.0	39.0
14	38.863067535879985	40.0	38.0	41.0	36.0	41.0
15	38.790752819105535	40.0	38.0	41.0	35.0	41.0
16	38.762734684176934	40.0	38.0	41.0	35.0	41.0
17	38.70411325402854	40.0	38.0	41.0	35.0	41.0
18	38.673134761709626	40.0	38.0	41.0	35.0	41.0
19	38.730637235879136	40.0	38.0	41.0	35.0	41.0
20	38.693689972988224	40.0	38.0	41.0	34.0	41.0
21	38.65434072481002	40.0	38.0	41.0	34.0	41.0
22	38.61893587185873	40.0	38.0	41.0	34.0	41.0
23	38.5519764603029	40.0	38.0	41.0	34.0	41.0
24	38.51507986711669	40.0	38.0	41.0	34.0	41.0
25	38.40030695385397	40.0	38.0	41.0	34.0	41.0
26	38.29593801888693	40.0	38.0	41.0	34.0	41.0
27	38.20952204393546	40.0	38.0	41.0	34.0	41.0
28	38.12399017490297	40.0	38.0	41.0	34.0	41.0
29	38.03989796890891	40.0	38.0	41.0	33.0	41.0
30	37.978361020037454	40.0	37.0	41.0	33.0	41.0
31	37.884141508421024	40.0	37.0	41.0	33.0	41.0
32	37.794293063116356	40.0	37.0	41.0	33.0	41.0
33	37.69882912768082	40.0	37.0	41.0	33.0	41.0
34	37.60847117034552	40.0	37.0	41.0	33.0	41.0
35	37.54695010602435	40.0	37.0	41.0	33.0	41.0
36	37.46064632827615	40.0	37.0	41.0	32.0	41.0
37	37.37852863220349	40.0	37.0	41.0	32.0	41.0
38	37.28679857143604	40.0	36.0	41.0	32.0	41.0
39	37.1808805912109	39.0	36.0	41.0	31.0	41.0
40	37.06787126517025	39.0	36.0	41.0	31.0	41.0
41	36.95857610891252	39.0	36.0	41.0	31.0	41.0
42	36.84687394061108	39.0	36.0	41.0	31.0	41.0
43	35.178408432404	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	9.0
10	12.0
11	18.0
12	26.0
13	16.0
14	10.0
15	19.0
16	29.0
17	48.0
18	95.0
19	214.0
20	485.0
21	1019.0
22	1990.0
23	3586.0
24	6259.0
25	10064.0
26	15922.0
27	24222.0
28	34888.0
29	49188.0
30	66574.0
31	87044.0
32	113542.0
33	148818.0
34	200672.0
35	279189.0
36	413622.0
37	696255.0
38	1368464.0
39	1451087.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.97834795047076	17.632132313880323	12.349534100108055	26.03998563554086
2	16.399833031258783	20.539105551027006	37.66369632278693	25.397365094927277
3	18.15465359012954	22.598145408379725	31.15798773712718	28.089213264363554
4	13.026175728165882	15.088211532344362	36.101862996357006	35.783749743132745
5	13.844752850472494	37.59291959240646	35.21132282915503	13.351004727966018
6	34.58997552170694	36.84079618995992	14.971088107780092	13.598140180553047
7	29.229744081798597	31.29714846183264	21.4293843268952	18.043723129473562
8	27.256400367878143	36.82814887080955	19.142371012424935	16.77307974888738
9	26.68059547358681	13.614366550273798	18.58824149181262	41.11679648432677
10	14.61638006782502	27.921259278889675	34.93841016965102	22.523950483634287
11	36.84940199694936	21.252161002584558	21.99893593620121	19.899501064264868
12	20.744136891847926	25.238620127213128	31.85332889906394	22.163914081875006
13	31.473607719167585	19.25635774098371	24.975600124341845	24.29443441550686
14	21.411086933529795	19.46283678765332	27.66612123008349	31.459955048733395
15	24.907899768889845	28.33514229541001	22.701736804663863	24.05522113103628
16	23.96914295411617	26.557842081833183	24.944816268031477	24.52819869601917
17	23.505495049047067	26.88854233313079	26.36477442128964	23.2411881965325
18	23.485126632037005	26.159702866417366	26.995270425420426	23.359900076125196
19	24.560691649512023	26.07925465668661	26.857416657383926	22.502637036417443
20	23.735117282270068	25.880717885159125	26.880680486091368	23.503484346479443
21	23.469181760675724	26.243549163487412	27.005967363080202	23.28130171275666
22	23.986515424300467	25.569541555793172	26.487487598991915	23.956455420914445
23	23.425469086855514	25.650070193626632	26.896645464478325	24.027815255039524
24	23.336093357724497	25.62141768203795	26.991510411618965	24.050978548618588
25	23.78602827128238	25.50720977619674	26.811532424790673	23.8952295277302
26	23.448009062638615	25.77405011394652	27.174243060964905	23.60369776244997
27	23.90144259866417	26.184756220409998	26.64209051941675	23.271710661509083
28	23.671357903850616	26.31933254326127	26.530536740964806	23.478772811923303
29	23.383385082115083	26.454311006626067	26.835841818833284	23.32646209242556
30	23.671458438979	26.27051268491929	26.58983235968413	23.46819651641759
31	23.51653380614334	25.64546568474677	26.920190791545235	23.91780971756465
32	22.986271325008754	26.252255505605238	26.94972801226368	23.81174515712233
33	23.415777500479553	25.711316193836552	26.836163531244107	24.036742774439787
34	23.62294018602216	25.663280509495944	27.406579742654202	23.307199561827694
35	23.432225047482742	25.789090169152363	26.989238317717547	23.789446465647348
36	23.248326190647578	25.418859505375213	27.378691298041215	23.954123005935994
37	22.979615899509913	25.192092469798244	27.418442887803202	24.40984874288865
38	23.021780332353046	25.12316558577999	27.72722648111367	24.12782760075329
39	22.6614624322343	24.9803855964528	28.2993920037576	24.058759967555304
40	23.04299324444151	24.836660576918824	28.381891130107334	23.738455048532327
41	22.542851087769982	24.678136786487116	28.630876429056585	24.14813569668632
42	22.08205838034691	24.8957350183557	28.616198300312906	24.40600830098448
43	21.819360089886448	24.392938734294905	28.576104891114422	25.211596284704225
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	402.0
1	606.0
2	810.0
3	3629.0
4	6448.0
5	6448.0
6	8199.5
7	9951.0
8	9176.0
9	8401.0
10	10895.0
11	13389.0
12	13389.0
13	20736.0
14	28083.0
15	45663.0
16	63243.0
17	50608.0
18	37973.0
19	37973.0
20	42049.5
21	46126.0
22	41027.5
23	35929.0
24	43649.0
25	51369.0
26	51369.0
27	62999.5
28	74630.0
29	85486.5
30	96343.0
31	110001.5
32	123660.0
33	123660.0
34	140989.0
35	158318.0
36	178812.5
37	199307.0
38	223010.5
39	246714.0
40	246714.0
41	269600.5
42	292487.0
43	315969.5
44	339452.0
45	363222.0
46	386992.0
47	386992.0
48	407084.5
49	427177.0
50	434121.0
51	441065.0
52	432232.0
53	423399.0
54	423399.0
55	395872.0
56	368345.0
57	338385.0
58	308425.0
59	282111.0
60	255797.0
61	255797.0
62	222275.5
63	188754.0
64	158452.5
65	128151.0
66	105706.0
67	83261.0
68	83261.0
69	67572.5
70	51884.0
71	42157.5
72	32431.0
73	26009.5
74	19588.0
75	19588.0
76	15457.5
77	11327.0
78	8966.0
79	6605.0
80	5077.0
81	3549.0
82	3549.0
83	2766.0
84	1983.0
85	1458.0
86	933.0
87	694.5
88	456.0
89	456.0
90	298.5
91	141.0
92	98.0
93	55.0
94	40.5
95	26.0
96	26.0
97	16.0
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4973386.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.03473519591279
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.45879386838774	42.14076091796562
2	10.71789332391654	11.368412685839104
3	3.6244821938515406	5.766703601196524
4	1.6775611954816902	3.5587605510924125
5	0.9948577797387957	2.638100945302038
6	0.657827819788147	2.0932634536181403
7	0.44837539977190743	1.6645629416685248
8	0.3481961619100668	1.4773192994506852
9	0.25910897412984946	1.2367598246875062
>10	1.6350300032290623	16.400334437424466
>50	0.12588173438092348	4.520642830694579
>100	0.049754527118256064	4.527625509574813
>500	0.0015166232742134542	0.5380008534221066
>1k	5.687329115203621E-4	0.6081584366862246
>5k	3.791552743427869E-5	0.1761897970577793
>10k+	1.1374658230283607E-4	1.2844039143196297
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	36487	0.7336450458500506	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14167	0.2848562327557121	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13220	0.26581487944028476	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8762	0.17617775897547466	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.010702567626965E-5	2.010702567626965E-5
2	0.0	0.0	0.0	2.010702567626965E-5	2.010702567626965E-5
3	0.0	0.0	0.0	4.02140513525393E-5	2.010702567626965E-5
4	0.0	0.0	0.0	4.02140513525393E-5	2.010702567626965E-5
5	0.0	4.02140513525393E-5	0.0	6.0321077028808944E-5	2.010702567626965E-5
6	0.0	4.02140513525393E-5	0.0	6.0321077028808944E-5	4.02140513525393E-5
7	0.0	4.02140513525393E-5	0.0	6.0321077028808944E-5	6.0321077028808944E-5
8	0.0	4.02140513525393E-5	0.0	6.0321077028808944E-5	6.0321077028808944E-5
9	0.0	4.02140513525393E-5	0.0	8.04281027050786E-5	8.04281027050786E-5
10	0.0	4.02140513525393E-5	0.0	8.04281027050786E-5	8.04281027050786E-5
11	0.0	4.02140513525393E-5	0.0	1.2064215405761789E-4	8.04281027050786E-5
12	0.0	4.02140513525393E-5	0.0	1.608562054101572E-4	1.0053512838134823E-4
13	0.0	4.02140513525393E-5	0.0	1.8096323108642684E-4	1.0053512838134823E-4
14	2.010702567626965E-5	4.02140513525393E-5	0.0	2.4128430811523577E-4	1.8096323108642684E-4
15	2.010702567626965E-5	4.02140513525393E-5	0.0	3.619264621728537E-4	2.4128430811523577E-4
16	2.010702567626965E-5	4.02140513525393E-5	0.0	4.222475392016626E-4	3.0160538514404473E-4
17	2.010702567626965E-5	4.02140513525393E-5	0.0	4.8256861623047155E-4	3.0160538514404473E-4
18	2.010702567626965E-5	4.02140513525393E-5	0.0	5.831037446118198E-4	3.217124108203144E-4
19	6.0321077028808944E-5	4.02140513525393E-5	0.0	7.238529243457074E-4	3.217124108203144E-4
20	6.0321077028808944E-5	4.02140513525393E-5	0.0	0.0010053512838134825	3.4181943649658404E-4
21	6.0321077028808944E-5	4.02140513525393E-5	2.010702567626965E-5	0.0017090971824829202	3.4181943649658404E-4
22	6.0321077028808944E-5	4.02140513525393E-5	2.010702567626965E-5	0.0029356257487353687	3.4181943649658404E-4
23	6.0321077028808944E-5	4.02140513525393E-5	2.010702567626965E-5	0.003619264621728537	3.820334878491233E-4
24	6.0321077028808944E-5	4.02140513525393E-5	2.010702567626965E-5	0.004906114265009794	3.820334878491233E-4
25	6.0321077028808944E-5	4.02140513525393E-5	2.010702567626965E-5	0.006012000677204625	3.820334878491233E-4
26	6.0321077028808944E-5	4.02140513525393E-5	2.010702567626965E-5	0.00796238216780278	3.820334878491233E-4
27	6.0321077028808944E-5	4.02140513525393E-5	2.010702567626965E-5	0.02002659757356457	3.820334878491233E-4
28	6.0321077028808944E-5	4.02140513525393E-5	2.010702567626965E-5	0.10373214546387512	3.820334878491233E-4
29	6.0321077028808944E-5	4.02140513525393E-5	2.010702567626965E-5	0.25192092469798244	4.0214051352539294E-4
30	6.0321077028808944E-5	4.02140513525393E-5	2.010702567626965E-5	0.431898911526272	4.0214051352539294E-4
31	6.0321077028808944E-5	4.02140513525393E-5	2.010702567626965E-5	0.9202583511515092	4.0214051352539294E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	5265	0.0	17.28775	37
AACGCAG	15765	0.0	15.572153	5
CGCGAAC	135	3.979585E-7	15.074075	14
TACGGAT	955	0.0	14.335079	27
TATACTG	865	0.0	14.329479	5
TTAGACT	570	0.0	14.280702	4
ACGCAGA	17485	0.0	14.146125	6
TCGTTAC	695	0.0	14.107915	23
CGCAGAG	17860	0.0	13.828387	7
GTCTTAT	555	0.0	13.666667	1
AGAGTAC	18040	0.0	13.6288805	10
GTTACGG	845	0.0	13.573965	25
CTCGTTA	775	0.0	13.367743	22
CAGAGTA	18600	0.0	13.337904	9
GTCTTAG	960	0.0	13.296874	1
CAATACT	755	0.0	13.231787	4
GCAGAGT	18890	0.0	13.1919	8
TTACGGA	1015	0.0	13.123153	26
TAGACAG	945	0.0	13.116403	5
GTACTAC	555	0.0	13.0	1
>>END_MODULE