FastQCFastQC Report
Thu 9 Feb 2017
SRR2727333.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727333.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4431272
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT221860.5006688824337572No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT92100.20784099915329052No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT84990.19179594482126125No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52210.11782169995432464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC45100.017.7616437
AACGCAG87750.016.8660985
GGTATCA49500.015.5848491
ACGCAGA99400.014.9079486
CGCAGAG101600.014.5851397
GGACCGT2950.014.4237296
GGAGTGT15950.014.3824466
TACTTAC10800.014.21759331
AGAGTAC105900.013.99291910
GTATAGA6700.013.805971
CAGAGTA109450.013.674289
TATACCG2850.013.6315785
CAACGCA109550.013.6280244
TCAACGC110200.013.5308533
CTTAGAC6900.013.4057973
CTATACT7600.013.3881584
ATCAACG110600.013.3815552
GTACCGT2651.8189894E-1213.2641526
GCAGAGT113750.013.2549458
CGTTACG4750.013.242105524
Produced by FastQC (version 0.11.5)