##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727333.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4431272
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33501983177742	33.0	31.0	34.0	30.0	34.0
2	32.482732046238645	34.0	31.0	34.0	31.0	34.0
3	32.57870471503442	34.0	31.0	34.0	31.0	34.0
4	36.11568867810416	37.0	35.0	37.0	35.0	37.0
5	35.91787279137909	37.0	35.0	37.0	35.0	37.0
6	35.92940198660791	37.0	35.0	37.0	35.0	37.0
7	35.8959738874075	37.0	35.0	37.0	35.0	37.0
8	35.849789857178706	37.0	35.0	37.0	35.0	37.0
9	37.693678022924345	39.0	37.0	39.0	35.0	39.0
10	37.50988068437234	39.0	37.0	39.0	35.0	39.0
11	37.62337698972214	39.0	37.0	39.0	35.0	39.0
12	37.5459723528594	39.0	37.0	39.0	35.0	39.0
13	37.62542448308296	39.0	37.0	39.0	35.0	39.0
14	38.8717970821922	40.0	38.0	41.0	36.0	41.0
15	38.795002879534366	40.0	38.0	41.0	35.0	41.0
16	38.766471568434525	40.0	38.0	41.0	35.0	41.0
17	38.699203073067956	40.0	38.0	41.0	35.0	41.0
18	38.658819634633126	40.0	38.0	41.0	35.0	41.0
19	38.71608987216312	40.0	38.0	41.0	34.0	41.0
20	38.67696950221065	40.0	38.0	41.0	34.0	41.0
21	38.6399304759446	40.0	38.0	41.0	34.0	41.0
22	38.610162499616365	40.0	38.0	41.0	34.0	41.0
23	38.548911012458724	40.0	38.0	41.0	34.0	41.0
24	38.507577959556535	40.0	38.0	41.0	34.0	41.0
25	38.393964532080176	40.0	38.0	41.0	34.0	41.0
26	38.29669494447644	40.0	38.0	41.0	34.0	41.0
27	38.21752264361113	40.0	38.0	41.0	34.0	41.0
28	38.135651117782885	40.0	38.0	41.0	34.0	41.0
29	38.064166225860205	40.0	38.0	41.0	33.0	41.0
30	38.01791539765557	40.0	38.0	41.0	33.0	41.0
31	37.93440912676992	40.0	37.0	41.0	33.0	41.0
32	37.85187909927443	40.0	37.0	41.0	33.0	41.0
33	37.76934794343475	40.0	37.0	41.0	33.0	41.0
34	37.69586294860708	40.0	37.0	41.0	33.0	41.0
35	37.6432071874622	40.0	37.0	41.0	33.0	41.0
36	37.56287562577968	40.0	37.0	41.0	33.0	41.0
37	37.501034465950184	40.0	37.0	41.0	32.0	41.0
38	37.42456567775573	40.0	37.0	41.0	32.0	41.0
39	37.32682489361971	40.0	37.0	41.0	32.0	41.0
40	37.234058301995454	39.0	36.0	41.0	31.0	41.0
41	37.141975712617054	39.0	36.0	41.0	31.0	41.0
42	37.05304097785015	39.0	36.0	41.0	31.0	41.0
43	35.369490746675	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	6.0
11	6.0
12	6.0
13	9.0
14	5.0
15	4.0
16	24.0
17	28.0
18	73.0
19	165.0
20	368.0
21	808.0
22	1644.0
23	3054.0
24	5207.0
25	8597.0
26	13489.0
27	20067.0
28	29334.0
29	41193.0
30	56245.0
31	74556.0
32	99120.0
33	131584.0
34	178596.0
35	247588.0
36	367168.0
37	614470.0
38	1237150.0
39	1300707.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.53546791982076	17.74959876080728	12.108306599098407	26.606626720273546
2	16.69026410475367	20.061214928805995	37.3538342940808	25.89468667235954
3	17.97677957931718	22.01406277926519	31.256172042700154	28.75298559871748
4	13.196210929954196	15.072647312103612	36.06045848686337	35.67068327107882
5	13.81989189560018	37.378138827858	35.34104880043473	13.460920476107086
6	34.86082551466035	36.72717449978246	15.009234368822316	13.402765616734879
7	29.612716168179247	31.50686755405671	21.083314226705106	17.79710205105893
8	27.322719074793874	36.6318971166744	19.05764304244921	16.987740766082517
9	27.024046368627335	13.398365074407529	18.339068330718582	41.23852022624655
10	14.578545392835284	27.70263256238841	35.08380889279647	22.635013151979837
11	37.227143808820585	20.90142514384132	22.097154045159044	19.77427700217906
12	20.895535187187786	24.727279210122962	31.77207357165166	22.60511203103759
13	31.173419280062248	19.30955716552719	24.830206766815486	24.686816787595074
14	21.50235869068746	19.359429978570486	27.111515609964815	32.02669572077724
15	24.937625133370283	28.15530168312846	22.363781776428983	24.54329140707228
16	24.297899113392273	26.63165339433012	24.56123207963763	24.509215412639982
17	23.741354626843037	26.68624268607298	25.86780951383711	23.70459317324687
18	23.789264121001825	26.241020636963835	26.394768815816317	23.574946426218023
19	24.896598538749146	25.88071777133067	26.322915858020004	22.899767831900185
20	24.112850666806278	25.594095781075954	26.358977738220542	23.93407581389723
21	23.86605922633501	25.83982657801191	26.673740632486563	23.620373563166513
22	24.564391443359828	25.152394165828685	26.017518220501923	24.265696170309564
23	23.850894280468452	25.433916040360423	26.49907295241637	24.216116726754755
24	23.775633723228907	25.282266581694827	26.53064402275464	24.411455672321626
25	24.14852890998341	25.17480308137257	26.376421939343825	24.30024606930019
26	23.794883275050598	25.43651123198937	26.694953503192764	24.07365198976727
27	24.243670891789083	25.89552164705755	26.0226183362249	23.838189124928462
28	23.996540948061863	26.021963896596734	25.87557252184023	24.10592263350117
29	23.758212088989346	26.193201410339967	26.30077774508087	23.747808755589816
30	24.33867747229238	25.848605095782883	25.830325919961584	23.982391511963154
31	23.961787947117667	25.366847261914867	26.413521896195945	24.257842894771525
32	23.47745748850443	25.937338082609234	26.33056603160447	24.254638397281862
33	23.68505025193669	25.305081701145856	26.475761361523283	24.53410668539417
34	24.053793132084873	25.24221036307408	27.026709260907477	23.67728724393357
35	23.858567020936654	25.284703805137664	26.591732577011744	24.264996596913935
36	23.63204064205492	24.945816912164272	27.045417207519645	24.37672523826116
37	23.45416846449507	24.685372507036355	27.15297097537682	24.70748805309175
38	23.463037249800962	24.509599049663393	27.40463686273377	24.622726837801878
39	22.9926079915654	24.426236981164777	28.099380945245517	24.481774082024305
40	23.44812053965543	24.201583653632635	28.120751784137827	24.229544022574103
41	22.7879940567855	24.12070394234432	28.530972596581748	24.56032940428843
42	22.36784381550038	24.36767591788543	28.456366478970374	24.80811378764382
43	21.98199523748486	23.819300643246454	28.513550962342187	25.6851531569265
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	412.0
1	538.5
2	665.0
3	2212.5
4	3760.0
5	3760.0
6	4812.0
7	5864.0
8	5314.0
9	4764.0
10	6294.5
11	7825.0
12	7825.0
13	12069.0
14	16313.0
15	27209.5
16	38106.0
17	31449.0
18	24792.0
19	24792.0
20	28224.5
21	31657.0
22	30194.5
23	28732.0
24	35674.0
25	42616.0
26	42616.0
27	52464.0
28	62312.0
29	71534.5
30	80757.0
31	94454.0
32	108151.0
33	108151.0
34	124381.5
35	140612.0
36	159526.5
37	178441.0
38	199242.5
39	220044.0
40	220044.0
41	241971.5
42	263899.0
43	286353.0
44	308807.0
45	330955.0
46	353103.0
47	353103.0
48	371276.5
49	389450.0
50	394095.0
51	398740.0
52	389897.5
53	381055.0
54	381055.0
55	357113.5
56	333172.0
57	307903.0
58	282634.0
59	259231.0
60	235828.0
61	235828.0
62	205346.0
63	174864.0
64	146673.0
65	118482.0
66	97567.0
67	76652.0
68	76652.0
69	62276.0
70	47900.0
71	38636.5
72	29373.0
73	23698.5
74	18024.0
75	18024.0
76	14362.5
77	10701.0
78	8510.5
79	6320.0
80	4830.5
81	3341.0
82	3341.0
83	2516.5
84	1692.0
85	1252.5
86	813.0
87	599.0
88	385.0
89	385.0
90	262.0
91	139.0
92	93.5
93	48.0
94	33.0
95	18.0
96	18.0
97	11.0
98	4.0
99	4.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4431272.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.54746985448913
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.93403630524782	43.05651965853656
2	10.945436853277382	11.940917735967247
3	3.757658276498775	6.149122546823656
4	1.788283839648261	3.901854353379343
5	1.021829402673412	2.786910426937929
6	0.6703992258848611	2.1941148938656387
7	0.4734286461040409	1.8077034361128241
8	0.35725348187930944	1.5589818826578328
9	0.2697670187741406	1.3243597491886054
>10	1.635813059960794	16.494863914243787
>50	0.107992670928418	3.999034432396109
>100	0.037066043852961784	3.319055013032626
>500	5.796983695067288E-4	0.20605646793004462
>1k	2.8984893656735665E-4	0.24149449339915122
>5k	1.2422097281452116E-4	0.5179076630790056
>10k+	4.140699093817372E-5	0.501103332449665
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	22186	0.5006688824337572	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9210	0.20784099915329052	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8499	0.19179594482126125	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5221	0.11782169995432464	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	2.256688372999897E-5	0.0	0.0	0.0
6	2.256688372999897E-5	2.256688372999897E-5	0.0	0.0	0.0
7	2.256688372999897E-5	2.256688372999897E-5	0.0	2.256688372999897E-5	0.0
8	4.513376745999794E-5	2.256688372999897E-5	0.0	2.256688372999897E-5	0.0
9	4.513376745999794E-5	2.256688372999897E-5	0.0	2.256688372999897E-5	0.0
10	4.513376745999794E-5	2.256688372999897E-5	0.0	6.770065118999691E-5	0.0
11	4.513376745999794E-5	2.256688372999897E-5	0.0	9.026753491999588E-5	0.0
12	4.513376745999794E-5	2.256688372999897E-5	0.0	2.0310195356999073E-4	4.513376745999794E-5
13	4.513376745999794E-5	2.256688372999897E-5	0.0	2.0310195356999073E-4	4.513376745999794E-5
14	4.513376745999794E-5	2.256688372999897E-5	0.0	2.4823572102998867E-4	4.513376745999794E-5
15	4.513376745999794E-5	2.256688372999897E-5	0.0	2.933694884899866E-4	1.1283441864999485E-4
16	4.513376745999794E-5	2.256688372999897E-5	0.0	3.836370234099825E-4	1.1283441864999485E-4
17	4.513376745999794E-5	2.256688372999897E-5	0.0	4.964714420599773E-4	1.3540130237999382E-4
18	4.513376745999794E-5	2.256688372999897E-5	0.0	5.416052095199753E-4	1.8053506983999176E-4
19	4.513376745999794E-5	2.256688372999897E-5	0.0	5.867389769799732E-4	1.8053506983999176E-4
20	6.770065118999691E-5	2.256688372999897E-5	0.0	7.22140279359967E-4	1.8053506983999176E-4
21	6.770065118999691E-5	2.256688372999897E-5	0.0	0.0010380766515799527	1.8053506983999176E-4
22	6.770065118999691E-5	2.256688372999897E-5	0.0	0.0017602169309399198	2.256688372999897E-4
23	6.770065118999691E-5	2.256688372999897E-5	0.0	0.002234121489269898	2.256688372999897E-4
24	6.770065118999691E-5	2.256688372999897E-5	0.0	0.003227064373389853	2.256688372999897E-4
25	6.770065118999691E-5	2.256688372999897E-5	0.0	0.00372353581544983	2.256688372999897E-4
26	6.770065118999691E-5	2.256688372999897E-5	0.0	0.004423109211079798	2.4823572102998867E-4
27	6.770065118999691E-5	2.256688372999897E-5	0.0	0.01405916856378936	2.4823572102998867E-4
28	6.770065118999691E-5	2.256688372999897E-5	0.0	0.08654399910454605	2.4823572102998867E-4
29	6.770065118999691E-5	2.256688372999897E-5	0.0	0.20770559785091053	2.4823572102998867E-4
30	6.770065118999691E-5	2.256688372999897E-5	0.0	0.35718863567842374	2.4823572102998867E-4
31	6.770065118999691E-5	2.256688372999897E-5	0.0	0.8400296799654817	2.4823572102998867E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	4510	0.0	17.76164	37
AACGCAG	8775	0.0	16.866098	5
GGTATCA	4950	0.0	15.584849	1
ACGCAGA	9940	0.0	14.907948	6
CGCAGAG	10160	0.0	14.585139	7
GGACCGT	295	0.0	14.423729	6
GGAGTGT	1595	0.0	14.382446	6
TACTTAC	1080	0.0	14.217593	31
AGAGTAC	10590	0.0	13.992919	10
GTATAGA	670	0.0	13.80597	1
CAGAGTA	10945	0.0	13.67428	9
TATACCG	285	0.0	13.631578	5
CAACGCA	10955	0.0	13.628024	4
TCAACGC	11020	0.0	13.530853	3
CTTAGAC	690	0.0	13.405797	3
CTATACT	760	0.0	13.388158	4
ATCAACG	11060	0.0	13.381555	2
GTACCGT	265	1.8189894E-12	13.264152	6
GCAGAGT	11375	0.0	13.254945	8
CGTTACG	475	0.0	13.2421055	24
>>END_MODULE