FastQCFastQC Report
Thu 9 Feb 2017
SRR2727332.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727332.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3327553
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT133400.4008951923530594No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT56590.17006490955966744No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT52420.1575331782844631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC28200.018.43439737
CGTTACG5050.018.3168324
CTCGTTA7900.016.62658122
TCGTTAC6950.015.97122323
TACGGAT12400.015.81451727
GTTACGG10600.015.53301925
AACGCAG61150.015.3990185
TAGATCG1455.3551048E-815.3103455
TACTTAC11000.015.13636331
TAAGACT6400.015.031254
ACGGATG13050.014.74329628
GGGTAAG22750.014.7186811
ACCCGAC20300.014.49014811
GGAGTGT14300.014.489516
GGTATCA37450.014.4739661
CACCCGA21150.014.17021310
GTATAAG4700.014.1702121
GACCGTG5500.014.1272727
CTTAGAC5650.014.0796453
TAGACAG6450.014.0542645
Produced by FastQC (version 0.11.5)