##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727332.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3327553
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40651193234187	33.0	31.0	34.0	31.0	34.0
2	32.554868096766604	34.0	31.0	34.0	31.0	34.0
3	32.64753829615937	34.0	31.0	34.0	31.0	34.0
4	36.1664382205182	37.0	35.0	37.0	35.0	37.0
5	35.97653771404993	37.0	35.0	37.0	35.0	37.0
6	35.98216437123616	37.0	35.0	37.0	35.0	37.0
7	35.95167860587044	37.0	35.0	37.0	35.0	37.0
8	35.904628716657555	37.0	35.0	37.0	35.0	37.0
9	37.75503290255632	39.0	37.0	39.0	35.0	39.0
10	37.574420302246125	39.0	37.0	39.0	35.0	39.0
11	37.68478067817402	39.0	37.0	39.0	35.0	39.0
12	37.60773667617015	39.0	37.0	39.0	35.0	39.0
13	37.685171055126695	39.0	37.0	39.0	35.0	39.0
14	38.962853484227	40.0	38.0	41.0	36.0	41.0
15	38.88946051347642	40.0	38.0	41.0	36.0	41.0
16	38.85244141866411	40.0	38.0	41.0	35.0	41.0
17	38.7888081722515	40.0	38.0	41.0	35.0	41.0
18	38.74379791997303	40.0	38.0	41.0	35.0	41.0
19	38.801163497621225	40.0	38.0	41.0	35.0	41.0
20	38.76439954525142	40.0	38.0	41.0	35.0	41.0
21	38.724109578419935	40.0	38.0	41.0	35.0	41.0
22	38.6999010984949	40.0	38.0	41.0	35.0	41.0
23	38.6353100912292	40.0	38.0	41.0	34.0	41.0
24	38.602021665770614	40.0	38.0	41.0	34.0	41.0
25	38.49515605010649	40.0	38.0	41.0	34.0	41.0
26	38.40161373838373	40.0	38.0	41.0	34.0	41.0
27	38.32478220482138	40.0	38.0	41.0	34.0	41.0
28	38.24070630881011	40.0	38.0	41.0	34.0	41.0
29	38.1739909176503	40.0	38.0	41.0	34.0	41.0
30	38.129872311575504	40.0	38.0	41.0	34.0	41.0
31	38.054011160753866	40.0	38.0	41.0	33.0	41.0
32	37.97581826645586	40.0	37.0	41.0	33.0	41.0
33	37.89822491181959	40.0	37.0	41.0	33.0	41.0
34	37.82432375983193	40.0	37.0	41.0	33.0	41.0
35	37.78350878257987	40.0	37.0	41.0	33.0	41.0
36	37.70319661324703	40.0	37.0	41.0	33.0	41.0
37	37.64150623596378	40.0	37.0	41.0	33.0	41.0
38	37.575412021987326	40.0	37.0	41.0	33.0	41.0
39	37.46775393209364	40.0	37.0	41.0	32.0	41.0
40	37.38129610557668	40.0	37.0	41.0	32.0	41.0
41	37.29123022232854	40.0	37.0	41.0	32.0	41.0
42	37.21205131819087	39.0	37.0	41.0	31.0	41.0
43	35.54636515180975	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	3.0
11	3.0
12	5.0
13	3.0
14	1.0
15	5.0
16	12.0
17	20.0
18	56.0
19	122.0
20	223.0
21	567.0
22	1089.0
23	2006.0
24	3479.0
25	5738.0
26	9206.0
27	13348.0
28	19581.0
29	27686.0
30	38554.0
31	51681.0
32	69537.0
33	92793.0
34	127695.0
35	179634.0
36	271032.0
37	454831.0
38	938804.0
39	1019836.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.78980590241538	17.46589761305079	11.910914717211115	26.833381767322717
2	16.681176828738717	19.726988570880764	37.72393707928919	25.867897521091326
3	18.14089813144975	21.877277386716305	31.05158655624719	28.93023792558676
4	13.318375394772076	14.835706598813001	35.972860537458004	35.87305746895692
5	13.858562132594132	37.47426412141294	35.300474552922225	13.366699193070705
6	35.25698914487613	36.40852602497992	14.895149679058456	13.439335151085496
7	29.884753150438176	31.517965303633027	20.847661930553773	17.749619615375025
8	27.307303595164374	37.16451698891047	18.786928412560222	16.741251003364933
9	27.50276254052152	13.282553275635278	17.931074275901842	41.28360990794136
10	14.513277474468476	27.705433993087414	34.99102794155345	22.79026059089066
11	37.77803088335482	20.81030715363512	21.67896349058903	19.732698472421024
12	20.927149770416882	24.30140105957741	31.817043935889227	22.954405234116482
13	31.325000683685577	19.186982145738927	24.609465273731175	24.878551896844318
14	21.545862680474208	19.04044804094781	26.836116509639368	32.577572768938616
15	25.3405129835648	27.874988016719794	22.125537895264177	24.65896110445123
16	24.42479503707379	26.56618842735187	24.43456197391897	24.57445456165537
17	24.061074308959167	26.47020197724875	25.43169109552876	24.03703261826333
18	23.969024685707485	26.400511126344195	26.0585481283093	23.57191605963902
19	25.33290979888224	25.79366880106793	25.85046729533684	23.022954104712984
20	24.299688089115335	25.376906092855624	25.952434116000557	24.370971702028488
21	23.957154100926417	25.809776733834145	26.51029149648405	23.722777668755388
22	24.798372858373703	24.839123524103147	25.58180140181088	24.780702215712267
23	23.84226487151369	25.321640256368568	26.36862583405884	24.467469038058898
24	23.868169793238454	24.981209916115535	26.387438457028335	24.763181833617676
25	24.34109990133891	25.034492313120182	25.95471807661666	24.669689708924246
26	23.907267592732556	25.244616689801784	26.44889502886956	24.399220688596092
27	24.583680560459893	25.763706844038246	25.674331858876474	23.978280736625383
28	24.16463389163148	26.184526587555478	25.322091038069118	24.328748482743926
29	24.000008414591743	26.109366252017622	26.00836109898174	23.88226423440889
30	24.88807841678254	25.63862994819316	25.41615415291657	24.057137482107724
31	24.328237596816642	25.244676794028525	26.040005974360135	24.3870796347947
32	23.817351669530133	25.927701226697213	25.949849634250754	24.305097469521897
33	23.987626943883388	25.05955577567059	26.04177904904896	24.911038231397065
34	24.29202480020604	25.284105166769695	26.68423312866842	23.739636904355844
35	24.187082820318714	25.18096631368456	26.07162079762516	24.560330068371563
36	23.96379561798114	24.91311182721958	26.669327280437006	24.453765274362272
37	23.67917205225582	24.848409627134412	26.80927997240014	24.663138348209632
38	24.128661511927834	24.543470832771106	26.67891390460197	24.648953750699086
39	23.282153582527464	24.683754097981307	27.535910021568398	24.498182297922828
40	24.169652594564234	24.24592485829677	27.31998558700643	24.264436960132567
41	23.29384385462831	24.42296185815823	27.593129245424493	24.690065041788966
42	22.956899559526175	24.55639324152012	27.766499887454838	24.72020731149887
43	22.58281085229897	24.019392027715263	27.470817144009423	25.926979975976337
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	200.0
1	309.0
2	418.0
3	1413.5
4	2409.0
5	2409.0
6	3091.0
7	3773.0
8	3451.0
9	3129.0
10	4121.0
11	5113.0
12	5113.0
13	7734.0
14	10355.0
15	16940.0
16	23525.0
17	19866.0
18	16207.0
19	16207.0
20	18592.5
21	20978.0
22	20380.0
23	19782.0
24	24807.5
25	29833.0
26	29833.0
27	36360.0
28	42887.0
29	49668.5
30	56450.0
31	66647.0
32	76844.0
33	76844.0
34	88230.0
35	99616.0
36	113948.0
37	128280.0
38	144896.0
39	161512.0
40	161512.0
41	177770.5
42	194029.0
43	211587.0
44	229145.0
45	248463.0
46	267781.0
47	267781.0
48	283205.5
49	298630.0
50	304989.5
51	311349.0
52	302704.5
53	294060.0
54	294060.0
55	275941.5
56	257823.0
57	237555.0
58	217287.0
59	199009.5
60	180732.0
61	180732.0
62	157287.5
63	133843.0
64	112204.5
65	90566.0
66	74727.0
67	58888.0
68	58888.0
69	48031.5
70	37175.0
71	30289.0
72	23403.0
73	18703.0
74	14003.0
75	14003.0
76	11099.0
77	8195.0
78	6405.0
79	4615.0
80	3545.0
81	2475.0
82	2475.0
83	1862.5
84	1250.0
85	922.0
86	594.0
87	424.0
88	254.0
89	254.0
90	175.0
91	96.0
92	60.0
93	24.0
94	20.0
95	16.0
96	16.0
97	11.0
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3327553.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.67286175532637
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.17877473148705	44.08108979582207
2	10.839964452044068	12.069836847426032
3	3.661840153305322	6.115953618752109
4	1.7728210032320404	3.9479207451950558
5	1.0099034288662714	2.8112106990751005
6	0.6826384781932169	2.2802662575190404
7	0.4901523459793353	1.9101728657729504
8	0.3488381207607316	1.5536653177680047
9	0.27753654913887726	1.3906128539033151
>10	1.6130672571164244	16.39347471921934
>50	0.09315765123704285	3.4743419681010517
>100	0.030064466563832222	2.695440021996019
>500	7.01639435435709E-4	0.24780506469006175
>1k	3.7780584815459847E-4	0.2998179854657789
>5k	1.0794452804417098E-4	0.3275522007980012
>10k+	5.397226402208549E-5	0.40083903849604036
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13340	0.4008951923530594	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5659	0.17006490955966744	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5242	0.1575331782844631	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.005211336979456E-5	0.0
2	0.0	0.0	0.0	3.005211336979456E-5	0.0
3	0.0	0.0	0.0	3.005211336979456E-5	0.0
4	0.0	0.0	0.0	3.005211336979456E-5	0.0
5	0.0	6.010422673958912E-5	0.0	3.005211336979456E-5	0.0
6	0.0	6.010422673958912E-5	0.0	3.005211336979456E-5	0.0
7	0.0	6.010422673958912E-5	0.0	3.005211336979456E-5	0.0
8	0.0	6.010422673958912E-5	0.0	3.005211336979456E-5	0.0
9	0.0	6.010422673958912E-5	0.0	3.005211336979456E-5	0.0
10	0.0	6.010422673958912E-5	0.0	3.005211336979456E-5	0.0
11	0.0	6.010422673958912E-5	0.0	3.005211336979456E-5	0.0
12	0.0	6.010422673958912E-5	0.0	6.010422673958912E-5	0.0
13	0.0	6.010422673958912E-5	0.0	6.010422673958912E-5	0.0
14	0.0	6.010422673958912E-5	0.0	6.010422673958912E-5	6.010422673958912E-5
15	0.0	6.010422673958912E-5	0.0	9.015634010938369E-5	1.2020845347917824E-4
16	0.0	6.010422673958912E-5	0.0	1.502605668489728E-4	1.502605668489728E-4
17	0.0	6.010422673958912E-5	0.0	1.8031268021876737E-4	1.502605668489728E-4
18	0.0	6.010422673958912E-5	0.0	2.1036479358856194E-4	1.502605668489728E-4
19	0.0	6.010422673958912E-5	0.0	2.7046902032815107E-4	1.502605668489728E-4
20	0.0	6.010422673958912E-5	0.0	4.207295871771239E-4	1.502605668489728E-4
21	0.0	6.010422673958912E-5	0.0	7.51302834244864E-4	1.8031268021876737E-4
22	0.0	6.010422673958912E-5	0.0	0.0013523451016407553	2.1036479358856194E-4
23	0.0	6.010422673958912E-5	0.0	0.0015927620085991118	3.005211336979456E-4
24	0.0	6.010422673958912E-5	0.0	0.002344064842843976	3.005211336979456E-4
25	0.0	6.010422673958912E-5	0.0	0.0028549507701304835	3.3057324706774015E-4
26	0.0	6.010422673958912E-5	0.0	0.0036062536043753473	3.3057324706774015E-4
27	0.0	6.010422673958912E-5	0.0	0.01093896926660522	3.3057324706774015E-4
28	0.0	6.010422673958912E-5	0.0	0.07005147626499113	3.3057324706774015E-4
29	0.0	6.010422673958912E-5	0.0	0.17048563914684453	3.3057324706774015E-4
30	0.0	6.010422673958912E-5	0.0	0.2910547179864603	3.3057324706774015E-4
31	0.0	6.010422673958912E-5	0.0	0.6966680921385775	3.3057324706774015E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2820	0.0	18.434397	37
CGTTACG	505	0.0	18.31683	24
CTCGTTA	790	0.0	16.626581	22
TCGTTAC	695	0.0	15.971223	23
TACGGAT	1240	0.0	15.814517	27
GTTACGG	1060	0.0	15.533019	25
AACGCAG	6115	0.0	15.399018	5
TAGATCG	145	5.3551048E-8	15.310345	5
TACTTAC	1100	0.0	15.136363	31
TAAGACT	640	0.0	15.03125	4
ACGGATG	1305	0.0	14.743296	28
GGGTAAG	2275	0.0	14.718681	1
ACCCGAC	2030	0.0	14.490148	11
GGAGTGT	1430	0.0	14.48951	6
GGTATCA	3745	0.0	14.473966	1
CACCCGA	2115	0.0	14.170213	10
GTATAAG	470	0.0	14.170212	1
GACCGTG	550	0.0	14.127272	7
CTTAGAC	565	0.0	14.079645	3
TAGACAG	645	0.0	14.054264	5
>>END_MODULE