FastQCFastQC Report
Thu 9 Feb 2017
SRR2727331.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727331.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1133346
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT54130.477612309038899No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22240.19623310092416615No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21010.18538028104391774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6950.028.2158281
TATTACG500.00703482418.4999982
GTATCAA10550.018.2369652
TATCAAC20150.017.7196031
ATCAACG20400.017.5931382
TCAACGC20400.017.502453
CTTATAC10750.017.38139537
CAACGCA20550.017.3746974
AACGCAG21300.016.8497665
GGGTAAG5950.015.8571431
ATCGTGT700.00259246415.8571428
ACGGAAT700.00259246415.85714225
ACCTAAG957.059537E-515.5789481
ACGCAGA23650.015.2537016
ACCCGAC6200.015.21774211
ACCGTGC1101.4517538E-515.1363648
GGGCGTC2750.014.811
ACGCTCG750.004104359514.825
GTTATAC1001.0933425E-414.7999991
GGTAAGA5900.014.7372882
Produced by FastQC (version 0.11.5)