##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2727331.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1133346 Sequences flagged as poor quality 0 Sequence length 43 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.835875363745934 31.0 31.0 33.0 28.0 34.0 2 31.0164345222024 31.0 31.0 34.0 28.0 34.0 3 31.07275712800857 31.0 31.0 34.0 28.0 34.0 4 34.94787470022394 35.0 35.0 37.0 32.0 37.0 5 33.729920959706924 35.0 33.0 37.0 28.0 37.0 6 33.73296063161647 35.0 33.0 37.0 29.0 37.0 7 33.772018430382246 35.0 33.0 37.0 29.0 37.0 8 33.62732122405691 35.0 33.0 37.0 28.0 37.0 9 35.25901975213218 37.0 34.0 39.0 30.0 39.0 10 34.71428054627625 37.0 33.0 39.0 28.0 39.0 11 34.98649573916527 37.0 33.0 39.0 29.0 39.0 12 34.784865345622606 37.0 33.0 39.0 28.0 39.0 13 35.00234526790583 37.0 34.0 39.0 29.0 39.0 14 35.76691142863697 38.0 34.0 40.0 29.0 40.0 15 35.62170069863925 38.0 34.0 40.0 29.0 40.0 16 35.54764123224505 38.0 34.0 40.0 27.0 40.0 17 35.36685178224479 38.0 33.0 39.0 27.0 40.0 18 35.27039756614485 37.0 33.0 39.0 27.0 40.0 19 35.31806968039769 38.0 34.0 40.0 27.0 40.0 20 35.226203648312165 37.0 33.0 39.0 27.0 40.0 21 35.137766401434334 37.0 33.0 39.0 27.0 40.0 22 35.07981234327381 37.0 33.0 39.0 27.0 40.0 23 34.956053138229635 37.0 33.0 39.0 27.0 40.0 24 34.84653495049173 37.0 33.0 39.0 27.0 40.0 25 34.66880017223337 37.0 33.0 39.0 26.0 40.0 26 34.4280263926462 37.0 32.0 39.0 25.0 40.0 27 34.2593753363933 37.0 32.0 39.0 25.0 40.0 28 34.10634528202332 37.0 32.0 39.0 24.0 40.0 29 33.96073308592433 37.0 32.0 39.0 24.0 40.0 30 33.85146371893491 36.0 32.0 39.0 24.0 40.0 31 33.69619516017174 36.0 31.0 39.0 24.0 40.0 32 33.55822758451523 36.0 31.0 39.0 24.0 40.0 33 33.426506997862965 36.0 31.0 39.0 24.0 40.0 34 33.27092079559111 36.0 31.0 39.0 23.0 40.0 35 33.185038814272076 36.0 30.0 39.0 23.0 40.0 36 33.0751023959144 36.0 30.0 38.0 22.0 40.0 37 32.94769470223568 36.0 30.0 38.0 21.0 40.0 38 32.819754073336824 35.0 30.0 38.0 21.0 40.0 39 32.63042707169743 35.0 30.0 38.0 20.0 40.0 40 32.47526968816231 35.0 30.0 38.0 19.0 40.0 41 32.35394045595961 35.0 30.0 38.0 19.0 40.0 42 32.24374639342266 35.0 30.0 38.0 19.0 40.0 43 30.523855027502634 33.0 27.0 38.0 15.0 39.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 1.0 12 6.0 13 7.0 14 21.0 15 42.0 16 96.0 17 195.0 18 414.0 19 795.0 20 1421.0 21 2525.0 22 4173.0 23 6829.0 24 9924.0 25 14285.0 26 20156.0 27 27174.0 28 35874.0 29 46318.0 30 57780.0 31 71704.0 32 87180.0 33 104069.0 34 122682.0 35 138945.0 36 143687.0 37 126881.0 38 85591.0 39 24569.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.11498165608737 19.687721137234348 13.001678216537579 26.1956189901407 2 17.95744635795247 21.283703299786648 35.81686439975083 24.941985942510055 3 18.669585457574296 22.68971699728062 31.450413201264222 27.19028434388086 4 14.826363705346823 16.274376933434272 35.23804733947091 33.66121202174799 5 13.933256040079552 37.617638391100336 34.79105233529743 13.658053233522685 6 34.423732911220405 37.170113981079034 14.891127687396436 13.515025420304125 7 29.092616023703265 32.174022760922085 20.78182655605614 17.95153465931851 8 27.127020345066732 36.75832446578538 19.032581400560815 17.082073788587067 9 27.20351949007629 13.502584382880425 18.371088793713483 40.9228073333298 10 14.853098700661583 27.994628295330813 34.731494177418014 22.420778826589586 11 37.462434243381985 21.030912007454035 21.862696828682505 19.643956920481475 12 20.876060796967565 24.969691515212478 31.192504319069375 22.961743368750582 13 31.09509364307105 19.80921977930835 24.56019609192603 24.535490485694574 14 21.493612718446087 19.675809505658467 26.87872900244056 31.951848773454884 15 25.173424532314048 28.331595117466335 22.042165411092466 24.45281493912715 16 24.34066913369792 27.059697568086005 24.39140386078038 24.208229437435698 17 23.867115602825614 26.69467223601619 25.509861948601753 23.92835021255645 18 23.828380741627004 26.689995817693802 26.13350203733017 23.34812140334902 19 24.95539755732142 25.965856852188125 26.276265147624823 22.80248044286564 20 24.264169988688362 25.507744325210485 26.11091405448998 24.117171631611175 21 24.092289556763777 25.935063078706765 26.456704307422445 23.51594305710701 22 24.848810513294264 25.28442329173968 25.622713628494743 24.244052566471318 23 24.08840724721312 25.62633123512149 26.09873771999019 24.186523797675203 24 23.97379088116074 25.262364714747303 26.26479468758879 24.49904971650317 25 24.15158301171928 25.20368889994759 26.164648748043405 24.480079340289727 26 24.000614110783467 25.26509997829436 26.455204324186965 24.279081586735206 27 24.482285197988965 25.789741173481 25.81691734033561 23.911056288194427 28 24.164818157914706 26.282794486414563 25.38659862036836 24.16578873530237 29 24.14434779846578 26.239294972585604 26.006621102470028 23.609736126478587 30 24.758987987781314 25.79450582611135 25.428333448037932 24.0181727380694 31 24.404021366820018 25.40777485428104 26.097325971062673 24.090877807836264 32 23.963908638668155 25.747565174271582 26.04385598043316 24.244670206627102 33 24.1095834811258 25.02201445983839 26.148325401069044 24.720076657966764 34 24.275993385956273 25.207041803650434 26.828788384129826 23.68817642626347 35 24.08461317197043 24.96986798382842 26.445586784618293 24.499932059582864 36 24.06555456144902 24.60660733791799 26.897699378654004 24.430138721978988 37 23.525384128059745 24.443815039714263 27.189754938033044 24.84104589419295 38 23.848056992304205 24.093171899843473 27.17554921444996 24.883221893402368 39 23.122329809255074 24.23434679259467 28.01845155848258 24.624871839667676 40 23.710499706179753 23.81143975449686 28.06459810155063 24.413462437772754 41 23.016272171075734 23.807910382178083 28.333712740857603 24.84210470588858 42 22.508836665943146 24.129700903342847 28.426888170073394 24.934574260640616 43 22.309691832855986 23.465561267256422 28.2078906176931 26.016856282194496 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 187.0 1 231.5 2 276.0 3 615.5 4 955.0 5 955.0 6 1185.5 7 1416.0 8 1342.0 9 1268.0 10 1663.0 11 2058.0 12 2058.0 13 2837.5 14 3617.0 15 6197.0 16 8777.0 17 7389.5 18 6002.0 19 6002.0 20 6821.5 21 7641.0 22 7518.0 23 7395.0 24 9182.0 25 10969.0 26 10969.0 27 13564.5 28 16160.0 29 18433.5 30 20707.0 31 24172.0 32 27637.0 33 27637.0 34 31731.0 35 35825.0 36 40835.5 37 45846.0 38 50749.5 39 55653.0 40 55653.0 41 61099.0 42 66545.0 43 72587.5 44 78630.0 45 84569.0 46 90508.0 47 90508.0 48 95258.5 49 100009.0 50 101509.5 51 103010.0 52 100340.5 53 97671.0 54 97671.0 55 91812.0 56 85953.0 57 78969.0 58 71985.0 59 66088.5 60 60192.0 61 60192.0 62 52145.0 63 44098.0 64 37298.5 65 30499.0 66 25240.0 67 19981.0 68 19981.0 69 16258.5 70 12536.0 71 10130.5 72 7725.0 73 6371.5 74 5018.0 75 5018.0 76 3941.5 77 2865.0 78 2287.5 79 1710.0 80 1348.5 81 987.0 82 987.0 83 776.5 84 566.0 85 421.0 86 276.0 87 198.0 88 120.0 89 120.0 90 88.0 91 56.0 92 33.0 93 10.0 94 8.0 95 6.0 96 6.0 97 3.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 1133346.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.97347724856664 #Duplication Level Percentage of deduplicated Percentage of total 1 86.38785582337228 63.04022231479314 2 7.951099252515341 11.604387208090468 3 2.4081526150400343 5.271938101941006 4 1.0907216640243567 3.1837501013680063 5 0.5952416489566057 2.1718426463767084 6 0.391576396465888 1.7144814755147513 7 0.24090394403365728 1.2305718935321044 8 0.1830188211148939 1.068441582294976 9 0.13542911297243912 0.8894459964859065 >10 0.5902871573689141 7.104938299634465 >50 0.01893081929737439 0.9235448061763485 >100 0.006177145405482438 0.7851299087867325 >500 1.2111988654495627E-4 0.05223576717516168 >1k 3.633596596348688E-4 0.48063976632576855 >5k 1.2111988654495627E-4 0.4784301315044842 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 5413 0.477612309038899 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2224 0.19623310092416615 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 2101 0.18538028104391774 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.823430796949917E-5 2 0.0 0.0 0.0 8.823430796949917E-5 8.823430796949917E-5 3 0.0 0.0 0.0 8.823430796949917E-5 8.823430796949917E-5 4 0.0 0.0 0.0 8.823430796949917E-5 8.823430796949917E-5 5 0.0 0.0 0.0 8.823430796949917E-5 8.823430796949917E-5 6 0.0 0.0 0.0 8.823430796949917E-5 8.823430796949917E-5 7 0.0 0.0 0.0 8.823430796949917E-5 8.823430796949917E-5 8 0.0 0.0 0.0 8.823430796949917E-5 8.823430796949917E-5 9 0.0 0.0 0.0 8.823430796949917E-5 1.7646861593899834E-4 10 0.0 0.0 0.0 1.7646861593899834E-4 1.7646861593899834E-4 11 0.0 0.0 0.0 1.7646861593899834E-4 1.7646861593899834E-4 12 0.0 0.0 0.0 1.7646861593899834E-4 1.7646861593899834E-4 13 0.0 0.0 0.0 1.7646861593899834E-4 1.7646861593899834E-4 14 0.0 0.0 0.0 1.7646861593899834E-4 2.647029239084975E-4 15 0.0 0.0 0.0 1.7646861593899834E-4 3.529372318779967E-4 16 0.0 0.0 0.0 1.7646861593899834E-4 3.529372318779967E-4 17 0.0 0.0 0.0 1.7646861593899834E-4 3.529372318779967E-4 18 0.0 0.0 0.0 1.7646861593899834E-4 4.4117153984749583E-4 19 0.0 0.0 0.0 1.7646861593899834E-4 4.4117153984749583E-4 20 0.0 0.0 0.0 3.529372318779967E-4 4.4117153984749583E-4 21 0.0 0.0 0.0 4.4117153984749583E-4 4.4117153984749583E-4 22 0.0 0.0 0.0 6.176401557864942E-4 5.29405847816995E-4 23 0.0 0.0 0.0 7.058744637559933E-4 6.176401557864942E-4 24 0.0 0.0 0.0 8.823430796949917E-4 6.176401557864942E-4 25 0.0 0.0 0.0 0.00105881169563399 6.176401557864942E-4 26 0.0 0.0 0.0 0.0017646861593899833 6.176401557864942E-4 27 0.0 0.0 0.0 0.010852819880248396 6.176401557864942E-4 28 0.0 0.0 0.0 0.06882276021620935 6.176401557864942E-4 29 0.0 0.0 0.0 0.16561579605874993 6.176401557864942E-4 30 0.0 0.0 0.0 0.28429094027772633 6.176401557864942E-4 31 0.0 0.0 0.0 0.744344622030695 7.058744637559933E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 695 0.0 28.215828 1 TATTACG 50 0.007034824 18.499998 2 GTATCAA 1055 0.0 18.236965 2 TATCAAC 2015 0.0 17.719603 1 ATCAACG 2040 0.0 17.593138 2 TCAACGC 2040 0.0 17.50245 3 CTTATAC 1075 0.0 17.381395 37 CAACGCA 2055 0.0 17.374697 4 AACGCAG 2130 0.0 16.849766 5 GGGTAAG 595 0.0 15.857143 1 ATCGTGT 70 0.002592464 15.857142 8 ACGGAAT 70 0.002592464 15.857142 25 ACCTAAG 95 7.059537E-5 15.578948 1 ACGCAGA 2365 0.0 15.253701 6 ACCCGAC 620 0.0 15.217742 11 ACCGTGC 110 1.4517538E-5 15.136364 8 GGGCGTC 275 0.0 14.8 11 ACGCTCG 75 0.0041043595 14.8 25 GTTATAC 100 1.0933425E-4 14.799999 1 GGTAAGA 590 0.0 14.737288 2 >>END_MODULE