FastQCFastQC Report
Thu 9 Feb 2017
SRR2727330.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727330.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3088037
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT506241.6393585957681207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT179680.5818583132261692No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT168620.5460426801880935No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136540.44215791455866627No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA66150.21421375456317396No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56570.18319081021373773No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45440.14714849595390211No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT43040.13937656835070306No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39810.12891684911806434No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA39570.12813965635774444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG1850.018.05
AGTCGGT4800.017.72916611
CTTATAC38450.016.98439437
TACCGAC1655.4569682E-1116.8181827
GTACTAG2250.016.4444451
TCTATAC3350.016.0149253
GTATTAA5050.015.0198021
AACGCAG204950.014.7855585
CGGTGAT5650.014.73451314
CGTACAC1956.7484507E-1014.230773
ATACCGA1702.4410838E-814.1470596
TATACAC14200.013.80985937
TAGACAG6700.013.805975
TACACCG2151.9826984E-1013.7674425
ACGTTTA1356.57509E-613.70370426
ATAATAC2501.0913936E-1113.323
TAATACC3200.013.2968754
GAGTACT156400.013.25991111
AGTACTT158250.013.1516612
CTATACA3800.013.1447374
Produced by FastQC (version 0.11.5)