##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727330.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3088037
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28994924607445	33.0	31.0	34.0	30.0	34.0
2	32.412398232275066	34.0	31.0	34.0	31.0	34.0
3	32.50428994212181	34.0	31.0	34.0	31.0	34.0
4	36.04327020693081	37.0	35.0	37.0	35.0	37.0
5	35.84775247187777	37.0	35.0	37.0	35.0	37.0
6	35.87986899120703	37.0	35.0	37.0	35.0	37.0
7	35.83439382364914	37.0	35.0	37.0	35.0	37.0
8	35.80019151324935	37.0	35.0	37.0	35.0	37.0
9	37.62702713730438	39.0	37.0	39.0	35.0	39.0
10	37.46801026023976	39.0	37.0	39.0	35.0	39.0
11	37.58021390287746	39.0	37.0	39.0	35.0	39.0
12	37.511090378774604	39.0	37.0	39.0	35.0	39.0
13	37.587356628175115	39.0	37.0	39.0	35.0	39.0
14	38.74610925970123	40.0	38.0	41.0	35.0	41.0
15	38.67520207821344	40.0	38.0	41.0	35.0	41.0
16	38.65001390851211	40.0	38.0	41.0	35.0	41.0
17	38.60814718217431	40.0	38.0	41.0	34.0	41.0
18	38.61043763400503	40.0	38.0	41.0	34.0	41.0
19	38.67346149026064	40.0	38.0	41.0	34.0	41.0
20	38.639580095704815	40.0	38.0	41.0	34.0	41.0
21	38.592887002325426	40.0	38.0	41.0	34.0	41.0
22	38.541220199110306	40.0	38.0	41.0	34.0	41.0
23	38.46155664585625	40.0	38.0	41.0	34.0	41.0
24	38.41871842856805	40.0	38.0	41.0	34.0	41.0
25	38.29783127598536	40.0	38.0	41.0	34.0	41.0
26	38.170167650193314	40.0	38.0	41.0	34.0	41.0
27	38.05316840439412	40.0	37.0	41.0	33.0	41.0
28	37.94026917423593	40.0	37.0	41.0	33.0	41.0
29	37.80828144222365	40.0	37.0	41.0	33.0	41.0
30	37.70101005914113	40.0	37.0	41.0	33.0	41.0
31	37.55709792337333	39.0	36.0	41.0	33.0	41.0
32	37.41904452569707	39.0	36.0	41.0	33.0	41.0
33	37.269368534120545	39.0	36.0	41.0	32.0	41.0
34	37.12499429249067	39.0	35.0	41.0	32.0	41.0
35	37.00058775202499	39.0	35.0	41.0	31.0	41.0
36	36.86523574685148	39.0	35.0	41.0	31.0	41.0
37	36.724552847002805	39.0	35.0	41.0	31.0	41.0
38	36.58020969308334	39.0	35.0	41.0	30.0	41.0
39	36.421755309279	39.0	35.0	41.0	30.0	41.0
40	36.23372776945354	39.0	35.0	40.0	30.0	41.0
41	36.06089143361948	39.0	35.0	40.0	29.0	41.0
42	35.87096203834345	39.0	35.0	40.0	28.0	41.0
43	34.22120525110289	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	17.0
11	17.0
12	17.0
13	7.0
14	7.0
15	11.0
16	20.0
17	35.0
18	88.0
19	183.0
20	391.0
21	833.0
22	1563.0
23	2987.0
24	5031.0
25	8214.0
26	12711.0
27	19225.0
28	27984.0
29	38483.0
30	50119.0
31	63294.0
32	79280.0
33	103153.0
34	138480.0
35	187349.0
36	278514.0
37	470809.0
38	813184.0
39	786029.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.28854414632985	17.35290088816941	13.117297493520965	24.24125747197977
2	16.28532948277498	22.736482755873716	36.140175781572566	24.83801197977874
3	19.06764718168856	23.793011547465266	31.042536083602624	26.09680518724355
4	13.242911273407671	14.959244335479141	34.69404673583898	37.10379765527421
5	14.673820294251655	37.81823210019828	33.80027506147109	13.707672544078973
6	34.78844327318617	35.64737080546638	14.838811840661236	14.72537408068621
7	28.731488644728024	30.901637512762964	21.772083689411755	18.59479015309726
8	28.482301215950457	35.40171312714194	19.509351733803708	16.6066339231039
9	26.088709429323547	13.964308070142941	19.356374292147407	40.5906082083861
10	15.052928446129368	28.45943879558438	33.93495609022819	22.55267666805806
11	35.593453057719195	21.86563826793526	21.811429072902946	20.7294796014426
12	20.759239607556516	26.260857625734406	31.527633898168965	21.452268868540113
13	32.10764637858938	18.940576165376257	25.43444265726091	23.517334798773458
14	21.520564682353225	19.960609280264453	28.922159935259845	29.596666102122484
15	24.874021911006896	28.501990099211895	23.67371245875616	22.95027553102505
16	23.080520084441993	25.930259255313327	26.094085012582426	24.895135647662254
17	22.786514539819308	26.99980602564024	27.949697493909557	22.26398194063089
18	22.61394536399661	25.41838067354763	28.827731014880975	23.139942947574788
19	23.384596751917154	26.431289521466226	28.70250583137443	21.48160789524219
20	22.736126542525234	26.487344549304296	28.417049407115265	22.3594795010552
21	22.135259389702906	27.24672664220021	28.323818658908557	22.29419530918833
22	22.68078394138412	26.669304804314198	27.79921354569262	22.85069770860906
23	22.184157767539702	26.358136252901115	28.080103962484905	23.377602017074278
24	22.27991439221745	26.441004430970228	28.39865584512103	22.8804253316913
25	22.61316817123629	26.33358991488768	28.159571922227617	22.893669991648416
26	22.326092595393124	26.610982964258522	28.770056835458902	22.292867604889448
27	22.738458120806197	27.04828342406519	28.502443461655414	21.7108149934732
28	22.600603554944453	27.228430229301008	28.267407417722	21.903558798032535
29	22.04342111185844	27.340766966198913	28.64175526394276	21.97405665799989
30	21.80475816837687	27.531697320984172	28.669928501504355	21.993616009134605
31	21.853624163181983	26.831543793030978	28.491951359391095	22.822880684395944
32	21.47927631696123	27.258255001478286	28.512903180888056	22.749565500672432
33	22.18580930215538	26.98274016794488	28.189688141690013	22.641762388209727
34	22.27813332547505	26.96479996839416	28.570156380898286	22.186910325232503
35	21.98603837972149	27.05278466546871	28.61769467140452	22.343482283405283
36	21.80430480593335	26.766583431480907	28.719409773911387	22.709701988674357
37	21.3606572719174	26.367430182993274	28.833559960583372	23.43835258450595
38	21.38432926807548	26.419016352459508	29.327465959766673	22.86918841969834
39	21.267620821900774	26.13984223634626	29.630862583576555	22.961674358176406
40	21.40239899975292	26.19699828726145	29.897342551271244	22.503260161714383
41	21.12895668024703	25.7969059308551	30.074963480036022	22.999173908861845
42	20.496613220631748	26.1475493978861	30.012690910115392	23.34314647136676
43	20.285961599553374	25.57802901973001	30.07405675514898	24.061952625567635
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	195.0
1	401.0
2	607.0
3	4359.5
4	8112.0
5	8112.0
6	10962.0
7	13812.0
8	12736.0
9	11660.0
10	14626.5
11	17593.0
12	17593.0
13	29058.5
14	40524.0
15	64317.0
16	88110.0
17	68518.0
18	48926.0
19	48926.0
20	50392.5
21	51859.0
22	39386.5
23	26914.0
24	30921.5
25	34929.0
26	34929.0
27	40865.0
28	46801.0
29	52924.0
30	59047.0
31	64608.0
32	70169.0
33	70169.0
34	78264.0
35	86359.0
36	98499.5
37	110640.0
38	124367.5
39	138095.0
40	138095.0
41	152979.5
42	167864.0
43	182536.0
44	197208.0
45	211290.5
46	225373.0
47	225373.0
48	241357.5
49	257342.0
50	260418.0
51	263494.0
52	263918.0
53	264342.0
54	264342.0
55	243790.0
56	223238.0
57	200541.5
58	177845.0
59	162738.0
60	147631.0
61	147631.0
62	128311.5
63	108992.0
64	91703.5
65	74415.0
66	61672.0
67	48929.0
68	48929.0
69	39843.5
70	30758.0
71	24901.5
72	19045.0
73	15234.5
74	11424.0
75	11424.0
76	9183.5
77	6943.0
78	5567.0
79	4191.0
80	3250.5
81	2310.0
82	2310.0
83	1777.0
84	1244.0
85	949.5
86	655.0
87	473.0
88	291.0
89	291.0
90	186.0
91	81.0
92	63.0
93	45.0
94	32.0
95	19.0
96	19.0
97	11.0
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3088037.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.78838681456594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.13881185750527	44.45464609723289
2	10.00451347081536	10.962623078611353
3	3.274569641860666	5.382251645684906
4	1.6162532599809005	3.5420763519254717
5	0.9060086764693245	2.4819376911877136
6	0.6065780297471112	1.9940059036201208
7	0.40822575097818936	1.5656221246581798
8	0.30157999178590866	1.3218464996399988
9	0.2343473803443366	1.155556343095715
>10	1.3872110584671267	13.715635429026229
>50	0.08012956127379325	3.0065139272277457
>100	0.03652076734906024	3.681038358429408
>500	0.0030087440965779937	1.171169808782685
>1k	0.0018878394327843454	1.9650079302618715
>5k	1.1798996454902158E-4	0.3966604816293334
>10k+	2.3597992909804317E-4	3.2034083289863076
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	50624	1.6393585957681207	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17968	0.5818583132261692	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16862	0.5460426801880935	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13654	0.44215791455866627	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	6615	0.21421375456317396	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5657	0.18319081021373773	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4544	0.14714849595390211	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	4304	0.13937656835070306	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3981	0.12891684911806434	No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	3957	0.12813965635774444	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.476606335999212E-5	0.0
3	0.0	0.0	0.0	9.714909503998818E-5	0.0
4	0.0	0.0	0.0	1.619151583999803E-4	0.0
5	0.0	0.0	0.0	1.619151583999803E-4	0.0
6	0.0	0.0	0.0	1.619151583999803E-4	0.0
7	0.0	0.0	0.0	1.619151583999803E-4	0.0
8	3.238303167999606E-5	0.0	0.0	1.619151583999803E-4	0.0
9	3.238303167999606E-5	0.0	0.0	1.9429819007997636E-4	3.238303167999606E-5
10	3.238303167999606E-5	0.0	0.0	1.9429819007997636E-4	3.238303167999606E-5
11	6.476606335999212E-5	0.0	0.0	2.590642534399685E-4	3.238303167999606E-5
12	6.476606335999212E-5	0.0	0.0	2.9144728511996455E-4	3.238303167999606E-5
13	6.476606335999212E-5	0.0	0.0	3.5621334847995667E-4	3.238303167999606E-5
14	6.476606335999212E-5	0.0	0.0	4.5336244351994485E-4	3.238303167999606E-5
15	9.714909503998818E-5	0.0	0.0	8.095757919999015E-4	6.476606335999212E-5
16	1.2953212671998424E-4	0.0	0.0	0.0015220024889598149	6.476606335999212E-5
17	1.2953212671998424E-4	0.0	0.0	0.002202046154239732	6.476606335999212E-5
18	1.2953212671998424E-4	0.0	0.0	0.0026877916294396733	6.476606335999212E-5
19	1.2953212671998424E-4	0.0	0.0	0.00427456018175948	6.476606335999212E-5
20	1.619151583999803E-4	0.0	0.0	0.006347074209279228	6.476606335999212E-5
21	1.619151583999803E-4	0.0	0.0	0.01020065497919876	6.476606335999212E-5
22	1.619151583999803E-4	0.0	0.0	0.014896194572798189	6.476606335999212E-5
23	1.619151583999803E-4	0.0	0.0	0.017875433487357827	6.476606335999212E-5
24	1.619151583999803E-4	0.0	0.0	0.025064466520316953	6.476606335999212E-5
25	1.619151583999803E-4	0.0	0.0	0.032253499553276076	6.476606335999212E-5
26	1.619151583999803E-4	0.0	0.0	0.03953968168127519	6.476606335999212E-5
27	1.619151583999803E-4	0.0	0.0	0.056022644806393185	6.476606335999212E-5
28	1.619151583999803E-4	0.0	0.0	0.14782853961918202	6.476606335999212E-5
29	1.619151583999803E-4	0.0	0.0	0.3340957378425194	9.714909503998818E-5
30	1.619151583999803E-4	0.0	0.0	0.5550775460268125	9.714909503998818E-5
31	1.619151583999803E-4	0.0	0.0	1.0977847739518665	1.2953212671998424E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	185	0.0	18.0	5
AGTCGGT	480	0.0	17.729166	11
CTTATAC	3845	0.0	16.984394	37
TACCGAC	165	5.4569682E-11	16.818182	7
GTACTAG	225	0.0	16.444445	1
TCTATAC	335	0.0	16.014925	3
GTATTAA	505	0.0	15.019802	1
AACGCAG	20495	0.0	14.785558	5
CGGTGAT	565	0.0	14.734513	14
CGTACAC	195	6.7484507E-10	14.23077	3
ATACCGA	170	2.4410838E-8	14.147059	6
TATACAC	1420	0.0	13.809859	37
TAGACAG	670	0.0	13.80597	5
TACACCG	215	1.9826984E-10	13.767442	5
ACGTTTA	135	6.57509E-6	13.703704	26
ATAATAC	250	1.0913936E-11	13.32	3
TAATACC	320	0.0	13.296875	4
GAGTACT	15640	0.0	13.259911	11
AGTACTT	15825	0.0	13.15166	12
CTATACA	380	0.0	13.144737	4
>>END_MODULE