FastQCFastQC Report
Thu 9 Feb 2017
SRR2727329.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727329.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4637610
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT379250.8177703601639638No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT147490.31803019227576274No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT133910.2887478679750992No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96980.2091163336287441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC49500.017.07979837
AACGCAG138900.016.7152635
AGTCGGT3900.015.65384711
TACGGAT8000.015.49375127
TAGGCCG1802.0190782E-1015.4166675
TATACTG8100.015.0740745
GTTACGG6900.015.01449225
ACGCAGA157650.014.7389786
CGCAGAG160600.014.4567247
ACGGATG9100.013.82417728
AGAGTAC167200.013.80861210
TAAGACT7950.013.7295614
CAGAGTA172150.013.6479839
GTATAGA6050.013.4545461
GCAGAGT174150.013.3850148
TATACAG7900.013.3481015
TCTATAC5400.013.01851753
TCTTATA75200.012.84175537
TTACGGA9400.012.79255226
GTATTAG5950.012.7478991
Produced by FastQC (version 0.11.5)