##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727329.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4637610
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.332330877326896	33.0	31.0	34.0	30.0	34.0
2	32.4754597734609	34.0	31.0	34.0	31.0	34.0
3	32.56733015497206	34.0	31.0	34.0	31.0	34.0
4	36.1044100301664	37.0	35.0	37.0	35.0	37.0
5	35.909064798463	37.0	35.0	37.0	35.0	37.0
6	35.92462475283605	37.0	35.0	37.0	35.0	37.0
7	35.89119568053372	37.0	35.0	37.0	35.0	37.0
8	35.84664989078426	37.0	35.0	37.0	35.0	37.0
9	37.68490105895062	39.0	37.0	39.0	35.0	39.0
10	37.512521104620696	39.0	37.0	39.0	35.0	39.0
11	37.621837972576394	39.0	37.0	39.0	35.0	39.0
12	37.54723316535888	39.0	37.0	39.0	35.0	39.0
13	37.62748743426032	39.0	37.0	39.0	35.0	39.0
14	38.853394959903916	40.0	38.0	41.0	36.0	41.0
15	38.77644196040633	40.0	38.0	41.0	35.0	41.0
16	38.752595410135825	40.0	38.0	41.0	35.0	41.0
17	38.69610402772118	40.0	38.0	41.0	35.0	41.0
18	38.665785825026255	40.0	38.0	41.0	35.0	41.0
19	38.717614029640266	40.0	38.0	41.0	35.0	41.0
20	38.6845219843842	40.0	38.0	41.0	34.0	41.0
21	38.64151297758975	40.0	38.0	41.0	34.0	41.0
22	38.60378061113375	40.0	38.0	41.0	34.0	41.0
23	38.54037877268679	40.0	38.0	41.0	34.0	41.0
24	38.49960044074426	40.0	38.0	41.0	34.0	41.0
25	38.38237367954615	40.0	38.0	41.0	34.0	41.0
26	38.2751190807334	40.0	38.0	41.0	34.0	41.0
27	38.18523981102335	40.0	38.0	41.0	34.0	41.0
28	38.10066693835834	40.0	38.0	41.0	33.0	41.0
29	38.02035164664558	40.0	37.0	41.0	33.0	41.0
30	37.95714279553477	40.0	37.0	41.0	33.0	41.0
31	37.86272239364673	40.0	37.0	41.0	33.0	41.0
32	37.768611418381454	40.0	37.0	41.0	33.0	41.0
33	37.66845745976915	40.0	37.0	41.0	33.0	41.0
34	37.57369205258743	40.0	37.0	41.0	33.0	41.0
35	37.50455622615959	40.0	37.0	41.0	33.0	41.0
36	37.4183010214313	40.0	37.0	41.0	32.0	41.0
37	37.33361321887783	40.0	36.0	41.0	32.0	41.0
38	37.238934494276144	40.0	36.0	41.0	31.0	41.0
39	37.12804828349085	39.0	36.0	41.0	31.0	41.0
40	37.005288715523726	39.0	36.0	41.0	31.0	41.0
41	36.896000094876456	39.0	36.0	41.0	31.0	41.0
42	36.781215108644325	39.0	36.0	41.0	30.0	41.0
43	35.11405982823049	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	19.0
11	18.0
12	9.0
13	6.0
14	6.0
15	10.0
16	25.0
17	36.0
18	103.0
19	199.0
20	457.0
21	1055.0
22	1903.0
23	3457.0
24	5964.0
25	9819.0
26	15111.0
27	22811.0
28	33073.0
29	45861.0
30	62288.0
31	82163.0
32	107031.0
33	140960.0
34	190915.0
35	263648.0
36	389154.0
37	654711.0
38	1267583.0
39	1339209.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.63935302882304	18.013869212805734	12.608865342277594	25.737912416093632
2	16.59766560793167	20.966855772693265	37.128197498280365	25.3072811210947
3	18.15667984155632	22.512910744974242	31.44537811502045	27.885031298448986
4	13.117122828353397	15.269653981253276	35.98456963824039	35.62865355215294
5	13.972951584975881	37.49539094490481	35.04552991734967	13.48612755276964
6	34.42706480277557	36.788302595517955	15.109614650649798	13.675017951056686
7	29.119481802048902	31.48250930975222	21.36870068850119	18.029308199697688
8	27.318769797374078	36.38602642309293	19.33373008942106	16.961473690111934
9	26.47171711290945	13.689551299052743	18.940682808601846	40.89804877943596
10	14.731596662936298	27.92494409836101	34.86563121952903	22.477828019173668
11	36.531273651730096	21.328529134618908	22.346747570408034	19.793449643242962
12	20.75590659844187	25.457379986674173	31.74346268875563	22.04325072612833
13	31.234019246982818	19.38218177035154	25.166454272782744	24.217344709882894
14	21.343148733938385	19.770442102721013	27.78957264625529	31.096836517085308
15	24.63350734537833	28.581661674871324	22.810283745291215	23.974547234459127
16	23.750358482062957	26.667809496701967	25.08311393152939	24.49871808970569
17	23.35371020849101	26.97885333178081	26.623821321758406	23.043615137969773
18	23.31347396611617	26.04992657856094	27.214125379236286	23.422474076086605
19	24.300598799812832	26.079187340030746	27.279396068233424	22.340817791922994
20	23.64894848855337	26.065494942438022	27.13218662198848	23.153369947020124
21	23.40252845754602	26.286643335683678	27.19452476598938	23.116303440780918
22	23.87988640700706	25.741405594692097	26.749899193765753	23.628808804535094
23	23.230284564678787	25.847925979114244	27.128995323021986	23.79279413318498
24	23.306121040794718	25.812282619711446	27.181155810859476	23.700440528634363
25	23.649164116861918	25.650863267933268	27.102408352578163	23.597564262626655
26	23.220689104948455	25.992246006024654	27.377808828254206	23.40925606077268
27	23.5391074281796	26.364614532054226	26.94916131369391	23.147116726072266
28	23.447637899694023	26.3421676251345	26.898078967399158	23.312115507772322
29	23.153003378895594	26.600166896310817	27.11017097168585	23.136658753107742
30	23.343101295710504	26.431243679395205	26.920116180532645	23.305538844361642
31	23.28367413387499	25.861963382000642	27.146418090352576	23.707944393771793
32	22.71210817640983	26.440774450632976	27.193425061615788	23.653692311341402
33	23.09734971245965	25.978208603138253	27.151161913140605	23.773279771261492
34	23.326842921246072	25.794795163888296	27.692432093254933	23.185929821610703
35	23.156884688449438	25.870761016989352	27.386929905705742	23.585424388855465
36	22.972048102363072	25.504559460584225	27.757206837142405	23.7661855999103
37	22.814747251278135	25.139436045721826	27.77611743980197	24.26969926319807
38	22.5663218770013	25.14793180107857	28.2608067517536	24.024939570166527
39	22.393603601855265	24.961585816832375	28.68126039058912	23.96355019072324
40	22.533891379395854	24.943171159282475	28.84147222383944	23.68146523748224
41	22.086139196698298	24.716071424720923	29.176019544549884	24.02176983403089
42	21.69440724856122	24.963871476902973	29.04549972938647	24.296221545149333
43	21.29739240686474	24.492033612140737	29.184127168951246	25.026446812043275
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	580.0
1	736.0
2	892.0
3	3616.5
4	6341.0
5	6341.0
6	8190.0
7	10039.0
8	9211.0
9	8383.0
10	11146.0
11	13909.0
12	13909.0
13	21821.0
14	29733.0
15	48039.0
16	66345.0
17	52908.0
18	39471.0
19	39471.0
20	43722.5
21	47974.0
22	42007.0
23	36040.0
24	43727.5
25	51415.0
26	51415.0
27	62125.5
28	72836.0
29	82815.5
30	92795.0
31	105260.5
32	117726.0
33	117726.0
34	133713.5
35	149701.0
36	168058.5
37	186416.0
38	209233.0
39	232050.0
40	232050.0
41	255144.0
42	278238.0
43	299828.5
44	321419.0
45	340936.0
46	360453.0
47	360453.0
48	378073.0
49	395693.0
50	399626.0
51	403559.0
52	392077.5
53	380596.0
54	380596.0
55	357769.0
56	334942.0
57	308552.5
58	282163.0
59	257575.0
60	232987.0
61	232987.0
62	202466.0
63	171945.0
64	144276.0
65	116607.0
66	96590.5
67	76574.0
68	76574.0
69	62272.5
70	47971.0
71	38833.5
72	29696.0
73	23884.0
74	18072.0
75	18072.0
76	14369.5
77	10667.0
78	8437.5
79	6208.0
80	4900.0
81	3592.0
82	3592.0
83	2757.0
84	1922.0
85	1459.0
86	996.0
87	714.0
88	432.0
89	432.0
90	291.0
91	150.0
92	92.0
93	34.0
94	34.0
95	34.0
96	34.0
97	23.0
98	12.0
99	7.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4637610.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.584220604197974
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.28333346773199	41.69052297303411
2	10.705633711232268	11.258948095583525
3	3.5486823533785654	5.598140871728487
4	1.7304327218110336	3.639738239377365
5	1.0515655432860538	2.7647877253963573
6	0.6764190614580523	2.134138148915686
7	0.47131329541062983	1.734854960969486
8	0.3594860202958843	1.5122633756291173
9	0.264998812486696	1.2541280414085823
>10	1.7264172143677976	17.068031386354008
>50	0.13376330849978532	4.758844776109305
>100	0.04647670908916131	4.145377992737565
>500	8.620391281782512E-4	0.3001254521596845
>1k	4.5154400425177294E-4	0.5047130013080539
>5k	4.104945493195086E-5	0.2093365275290308
>10k+	1.2314836479585258E-4	1.4260484317596844
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	37925	0.8177703601639638	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14749	0.31803019227576274	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13391	0.2887478679750992	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9698	0.2091163336287441	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.1562830854685926E-5	0.0
2	0.0	0.0	0.0	2.1562830854685926E-5	0.0
3	0.0	0.0	0.0	2.1562830854685926E-5	0.0
4	0.0	0.0	0.0	2.1562830854685926E-5	0.0
5	0.0	0.0	0.0	2.1562830854685926E-5	0.0
6	0.0	0.0	0.0	2.1562830854685926E-5	0.0
7	0.0	0.0	0.0	2.1562830854685926E-5	0.0
8	0.0	0.0	0.0	2.1562830854685926E-5	2.1562830854685926E-5
9	0.0	0.0	0.0	6.468849256405778E-5	4.312566170937185E-5
10	0.0	0.0	0.0	6.468849256405778E-5	4.312566170937185E-5
11	0.0	0.0	0.0	1.2937698512811556E-4	4.312566170937185E-5
12	0.0	2.1562830854685926E-5	0.0	2.587539702562311E-4	4.312566170937185E-5
13	0.0	2.1562830854685926E-5	0.0	3.0187963196560296E-4	4.312566170937185E-5
14	0.0	2.1562830854685926E-5	0.0	3.450052936749748E-4	8.62513234187437E-5
15	2.1562830854685926E-5	2.1562830854685926E-5	0.0	3.6656812452966073E-4	1.5093981598280148E-4
16	2.1562830854685926E-5	2.1562830854685926E-5	0.0	5.606336022218341E-4	2.587539702562311E-4
17	2.1562830854685926E-5	2.1562830854685926E-5	0.0	7.546990799140074E-4	2.587539702562311E-4
18	2.1562830854685926E-5	2.1562830854685926E-5	0.0	8.84076065042123E-4	2.8031680111091704E-4
19	2.1562830854685926E-5	2.1562830854685926E-5	0.0	0.0012290813587170978	2.8031680111091704E-4
20	2.1562830854685926E-5	2.1562830854685926E-5	0.0	0.0018975291152123616	3.0187963196560296E-4
21	2.1562830854685926E-5	2.1562830854685926E-5	0.0	0.003148173304784145	3.0187963196560296E-4
22	2.1562830854685926E-5	2.1562830854685926E-5	0.0	0.0054553962062355394	3.234424628202889E-4
23	2.1562830854685926E-5	2.1562830854685926E-5	0.0	0.006576663410679208	4.0969378623903263E-4
24	2.1562830854685926E-5	2.1562830854685926E-5	0.0	0.00884076065042123	4.0969378623903263E-4
25	2.1562830854685926E-5	2.1562830854685926E-5	0.0	0.009918902193155527	4.0969378623903263E-4
26	2.1562830854685926E-5	2.1562830854685926E-5	0.0	0.011643928661530401	4.3125661709371855E-4
27	2.1562830854685926E-5	2.1562830854685926E-5	0.0	0.02397786791041075	4.3125661709371855E-4
28	2.1562830854685926E-5	2.1562830854685926E-5	0.0	0.10723195784035311	4.3125661709371855E-4
29	2.1562830854685926E-5	2.1562830854685926E-5	0.0	0.25519610316520797	4.3125661709371855E-4
30	2.1562830854685926E-5	2.1562830854685926E-5	0.0	0.4305666065063686	4.3125661709371855E-4
31	2.1562830854685926E-5	2.1562830854685926E-5	0.0	0.9485273664667793	4.528194479484045E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	4950	0.0	17.079798	37
AACGCAG	13890	0.0	16.715263	5
AGTCGGT	390	0.0	15.653847	11
TACGGAT	800	0.0	15.493751	27
TAGGCCG	180	2.0190782E-10	15.416667	5
TATACTG	810	0.0	15.074074	5
GTTACGG	690	0.0	15.014492	25
ACGCAGA	15765	0.0	14.738978	6
CGCAGAG	16060	0.0	14.456724	7
ACGGATG	910	0.0	13.824177	28
AGAGTAC	16720	0.0	13.808612	10
TAAGACT	795	0.0	13.729561	4
CAGAGTA	17215	0.0	13.647983	9
GTATAGA	605	0.0	13.454546	1
GCAGAGT	17415	0.0	13.385014	8
TATACAG	790	0.0	13.348101	5
TCTATAC	540	0.0	13.0185175	3
TCTTATA	7520	0.0	12.841755	37
TTACGGA	940	0.0	12.792552	26
GTATTAG	595	0.0	12.747899	1
>>END_MODULE