FastQCFastQC Report
Thu 9 Feb 2017
SRR2727328.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727328.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2825591
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT203700.7209111297424149No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT81870.28974469411885867No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT72550.2567604440982435No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51410.181944237506419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT1551.8189894E-1219.0967756
CTTATAC26250.018.39428537
TATACCG1005.883894E-616.6500025
TATAAGA4350.015.7356312
AACGCAG88500.015.4271195
GGCGTCA5350.014.52336412
AGGGCGT6000.014.18333310
TATACTG4600.014.0760875
ATAGGAC3550.014.0704223
GTATTAC2252.7284841E-1113.97777751
TAATACG1203.303709E-513.8749994
GGGTAAG14750.013.796611
ACGCAGA100450.013.7207576
TTAATAC5550.013.6666673
TAGACCG1501.3073131E-613.5666675
CGCAGAG101950.013.5370287
TATACAG5100.013.4215695
GTAAGAG15750.013.3904763
AGAGTAC103800.013.2244710
TAGGACT4350.013.18390754
Produced by FastQC (version 0.11.5)