FastQCFastQC Report
Thu 9 Feb 2017
SRR2727327.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727327.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4367389
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT572931.3118364313323132No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT209390.479439775115063No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT195460.44754428790290945No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156750.3589100947957693No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA67870.15540177437823835No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61030.13974024296896842No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49430.11317975110529425No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48300.11059239284616049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC74200.017.12870637
AACGCAG219250.016.1584955
TATACAC26650.015.8968137
TATACCG2201.8189894E-1114.2954545
GTACTAG3250.014.230771
ACGCAGA249650.014.1834566
CGCAGAG250550.014.1472767
GGACCGT2900.014.0344836
AGAGTAC258300.013.62969410
CAGAGTA263500.013.4519929
GCAGAGT266750.013.3227748
TAGACAG6700.012.9776125
TAGAGAG12550.012.9721135
GGGTAAG11300.012.9336271
GAGTACT180100.012.88117811
ATACCGA2753.6379788E-1212.7818186
GTCTTAG7450.012.664431
TATTCCG1901.0480835E-712.6578955
GTATAAG6450.012.6201551
GTATTAA8650.012.6184981
Produced by FastQC (version 0.11.5)