##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727327.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4367389
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17414729945054	33.0	31.0	34.0	30.0	34.0
2	32.31954080573084	34.0	31.0	34.0	30.0	34.0
3	32.4051418364611	34.0	31.0	34.0	30.0	34.0
4	35.97865772890851	37.0	35.0	37.0	35.0	37.0
5	35.759896130159234	37.0	35.0	37.0	35.0	37.0
6	35.776347836201445	37.0	35.0	37.0	35.0	37.0
7	35.7396442130527	37.0	35.0	37.0	33.0	37.0
8	35.70047916501141	37.0	35.0	37.0	33.0	37.0
9	37.51167345065896	39.0	37.0	39.0	35.0	39.0
10	37.33328105190538	39.0	37.0	39.0	34.0	39.0
11	37.440547887994406	39.0	37.0	39.0	35.0	39.0
12	37.37044284353878	39.0	37.0	39.0	34.0	39.0
13	37.452117042928855	39.0	37.0	39.0	35.0	39.0
14	38.60564836335852	40.0	38.0	41.0	34.0	41.0
15	38.51923700865666	40.0	38.0	41.0	34.0	41.0
16	38.49807150221792	40.0	38.0	41.0	34.0	41.0
17	38.441195872407974	40.0	38.0	41.0	34.0	41.0
18	38.4286616099459	40.0	38.0	41.0	34.0	41.0
19	38.48127199111414	40.0	38.0	41.0	34.0	41.0
20	38.44178409571485	40.0	38.0	41.0	34.0	41.0
21	38.396278188180624	40.0	38.0	41.0	34.0	41.0
22	38.342100966962185	40.0	38.0	41.0	34.0	41.0
23	38.2681329737287	40.0	38.0	41.0	34.0	41.0
24	38.218610249739605	40.0	38.0	41.0	34.0	41.0
25	38.095211120420004	40.0	37.0	41.0	33.0	41.0
26	37.95925918208797	40.0	37.0	41.0	33.0	41.0
27	37.84726915784236	40.0	37.0	41.0	33.0	41.0
28	37.74631982633102	40.0	37.0	41.0	33.0	41.0
29	37.63000341851848	40.0	37.0	41.0	33.0	41.0
30	37.53564635529375	39.0	37.0	41.0	33.0	41.0
31	37.40856287360709	39.0	36.0	41.0	32.0	41.0
32	37.29181531574128	39.0	36.0	41.0	32.0	41.0
33	37.163125153266634	39.0	36.0	41.0	31.0	41.0
34	37.03825603810423	39.0	36.0	41.0	31.0	41.0
35	36.94081452327695	39.0	35.0	40.0	31.0	41.0
36	36.82871367766874	39.0	35.0	40.0	31.0	41.0
37	36.71008215663867	39.0	35.0	40.0	30.0	41.0
38	36.584152911499295	39.0	35.0	40.0	30.0	41.0
39	36.4376246311011	39.0	35.0	40.0	30.0	41.0
40	36.2754645853621	39.0	35.0	40.0	30.0	41.0
41	36.12647030067622	39.0	35.0	40.0	29.0	41.0
42	35.96538961837381	39.0	35.0	40.0	28.0	41.0
43	34.289387091463574	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	10.0
10	37.0
11	34.0
12	20.0
13	9.0
14	14.0
15	22.0
16	30.0
17	53.0
18	125.0
19	288.0
20	675.0
21	1367.0
22	2605.0
23	4749.0
24	7843.0
25	12805.0
26	19626.0
27	28909.0
28	41108.0
29	56530.0
30	74090.0
31	94338.0
32	120363.0
33	154774.0
34	205729.0
35	279514.0
36	408869.0
37	673538.0
38	1171065.0
39	1008247.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.75726549661594	18.12155958628828	13.36446100862552	24.756713908470253
2	16.720195979794795	22.232734478197386	36.17142874152039	24.87564080048743
3	18.74584562996335	23.112253110496912	31.414375957809114	26.72752530173062
4	13.62461644703506	15.531156029380483	34.79930457305269	36.04492295053177
5	14.587411380117501	37.52017509775291	34.064494827458695	13.82791869467089
6	34.426747880713165	36.01852273749831	15.043610724851849	14.511118656936672
7	28.618678116375712	31.32308571551561	21.555052687086036	18.503183481022642
8	27.83125112052075	35.60795706542284	19.56631296181769	16.994478852238718
9	26.110886847954234	13.972284126740256	19.389502515118302	40.52732651018721
10	15.202332560713048	28.294182176123993	34.060671032509354	22.442814230653603
11	35.78359976635926	21.789197161049774	22.040354087991705	20.386848984599265
12	20.81493084311931	26.07429748071445	31.357957809574554	21.752813866591687
13	31.64165133904949	19.30180709801669	25.209364221964197	23.847177340969626
14	21.377120288575167	20.21319832055262	28.331779010296543	30.077902380575672
15	24.62347640661274	28.69208582061273	23.321760438559515	23.36267733421502
16	23.249337304279514	26.45592137544881	25.648345040938647	24.64639627933303
17	23.06410077050613	27.04771661054236	27.373792442120454	22.514390176831053
18	22.942998665793223	25.775972783738748	28.0126180653933	23.26841048507472
19	23.79405635724228	26.318218963321105	28.192908852405868	21.69481582703075
20	23.26355632621688	26.36815726741996	27.78053431924658	22.58775208711658
21	22.796938857518757	26.818243119630512	27.83006047778203	22.55475754506869
22	23.385528516008076	26.326484771564886	27.378280249366384	22.90970646306065
23	22.709678482956292	26.234003886532665	27.69437299951985	23.361944630991193
24	22.838084722931708	26.19411735478566	27.870084391383504	23.097713530899124
25	23.037265514933523	26.007964941982497	27.843363620689615	23.111405922394365
26	22.713822835566056	26.418507717082218	28.24183968957196	22.625829757779766
27	23.071313317865663	26.74156114786203	27.873083895206037	22.31404163906627
28	22.862676074881353	27.024979913628027	27.66614102842682	22.44620298306379
29	22.513886443364676	27.114758955522394	27.959199420981278	22.412155180131656
30	22.483204495866982	27.104043170873947	27.872786234521357	22.53996609873771
31	22.4865016603742	26.473666531650835	27.9068340374535	23.13299777052147
32	21.972121100272954	26.8252266972326	28.092780377474963	23.10987182501948
33	22.447393625802512	26.408890987269512	27.966091410680388	23.177623976247595
34	22.61921253178959	26.232607171012244	28.470282816575303	22.67789748062286
35	22.334053595867005	26.2495967270147	28.28781681686701	23.128532860251287
36	22.100985279763265	25.896456670106556	28.73506344408524	23.267494606044938
37	21.76149182039887	25.540614770060554	28.800159546126984	23.89773386341359
38	21.466029245391237	25.527174245298507	29.39426737577074	23.612529133539514
39	21.30664339723345	25.289709709851817	29.979216415116678	23.42443047779806
40	21.42316610679745	25.440371810250927	30.03155890166871	23.104903181282914
41	21.053311257595787	25.06458664433143	30.393193736578077	23.488908361494705
42	20.428223819769663	25.476113989388168	30.21079642779702	23.884865763045152
43	20.16614503539758	24.90957411854085	30.228152335411384	24.696128510650183
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	996.0
1	1099.0
2	1202.0
3	5290.0
4	9378.0
5	9378.0
6	12524.5
7	15671.0
8	14580.0
9	13489.0
10	17580.0
11	21671.0
12	21671.0
13	35090.0
14	48509.0
15	75239.0
16	101969.0
17	78856.0
18	55743.0
19	55743.0
20	58596.5
21	61450.0
22	48912.0
23	36374.0
24	42626.0
25	48878.0
26	48878.0
27	57553.0
28	66228.0
29	74619.5
30	83011.0
31	92690.5
32	102370.0
33	102370.0
34	115205.5
35	128041.0
36	146002.0
37	163963.0
38	184953.0
39	205943.0
40	205943.0
41	228489.5
42	251036.0
43	272468.0
44	293900.0
45	314295.5
46	334691.0
47	334691.0
48	352261.0
49	369831.0
50	371189.0
51	372547.0
52	365058.0
53	357569.0
54	357569.0
55	333421.0
56	309273.0
57	282514.0
58	255755.0
59	233306.5
60	210858.0
61	210858.0
62	183625.0
63	156392.0
64	131123.0
65	105854.0
66	88231.5
67	70609.0
68	70609.0
69	57849.0
70	45089.0
71	36759.0
72	28429.0
73	22906.0
74	17383.0
75	17383.0
76	13836.5
77	10290.0
78	8209.5
79	6129.0
80	4754.0
81	3379.0
82	3379.0
83	2625.5
84	1872.0
85	1410.5
86	949.0
87	687.0
88	425.0
89	425.0
90	288.5
91	152.0
92	100.5
93	49.0
94	38.5
95	28.0
96	28.0
97	19.5
98	11.0
99	7.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4367389.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.27328782264177
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.07590877259294	45.62407949842369
2	10.14239137471994	11.41491418078989
3	3.198407803784939	5.399547687497202
4	1.5846662150263842	3.5669751208398415
5	0.9175133158005703	2.5815745450575953
6	0.5979996422017032	2.019084359007194
7	0.41343660268970633	1.6285805857701123
8	0.32029522523227966	1.4419252318171156
9	0.23996657514297276	1.2153337335750691
>10	1.3940363632172426	14.303983197650414
>50	0.08248406898907819	3.150314925019346
>100	0.029724994364494835	2.926728818829042
>500	0.0017470384220455131	0.6777098227610442
>1k	0.0011782350472035862	1.1606482462240686
>5k	8.125758946229935E-5	0.29470611432731986
>10k+	1.625151789245987E-4	2.593893932410971
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	57293	1.3118364313323132	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	20939	0.479439775115063	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	19546	0.44754428790290945	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15675	0.3589100947957693	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	6787	0.15540177437823835	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6103	0.13974024296896842	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4943	0.11317975110529425	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4830	0.11059239284616049	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.2896975744546683E-5	2.2896975744546683E-5
2	0.0	0.0	0.0	2.2896975744546683E-5	2.2896975744546683E-5
3	0.0	0.0	0.0	2.2896975744546683E-5	2.2896975744546683E-5
4	0.0	0.0	0.0	2.2896975744546683E-5	2.2896975744546683E-5
5	0.0	0.0	0.0	2.2896975744546683E-5	2.2896975744546683E-5
6	0.0	0.0	0.0	2.2896975744546683E-5	4.5793951489093366E-5
7	0.0	0.0	0.0	2.2896975744546683E-5	9.158790297818673E-5
8	0.0	0.0	0.0	2.2896975744546683E-5	9.158790297818673E-5
9	0.0	0.0	0.0	4.5793951489093366E-5	9.158790297818673E-5
10	0.0	0.0	0.0	1.373818544672801E-4	9.158790297818673E-5
11	2.2896975744546683E-5	0.0	2.2896975744546683E-5	3.205576604236536E-4	9.158790297818673E-5
12	2.2896975744546683E-5	0.0	2.2896975744546683E-5	4.808364906354804E-4	9.158790297818673E-5
13	2.2896975744546683E-5	0.0	2.2896975744546683E-5	5.495274178691204E-4	9.158790297818673E-5
14	2.2896975744546683E-5	0.0	2.2896975744546683E-5	6.182183451027605E-4	1.1448487872273342E-4
15	2.2896975744546683E-5	0.0	2.2896975744546683E-5	8.929820540373207E-4	1.373818544672801E-4
16	4.5793951489093366E-5	0.0	2.2896975744546683E-5	0.0015798913263737212	1.602788302118268E-4
17	4.5793951489093366E-5	0.0	2.2896975744546683E-5	0.001969139914031015	1.602788302118268E-4
18	4.5793951489093366E-5	0.0	2.2896975744546683E-5	0.002243903622965575	2.5186673319001353E-4
19	4.5793951489093366E-5	0.0	2.2896975744546683E-5	0.003045297774024709	2.5186673319001353E-4
20	4.5793951489093366E-5	0.0	2.2896975744546683E-5	0.004739673979121163	2.747637089345602E-4
21	4.5793951489093366E-5	0.0	2.2896975744546683E-5	0.007258341311021299	2.747637089345602E-4
22	4.5793951489093366E-5	0.0	2.2896975744546683E-5	0.011219518114827876	2.747637089345602E-4
23	4.5793951489093366E-5	0.0	2.2896975744546683E-5	0.01373818544672801	3.205576604236536E-4
24	4.5793951489093366E-5	0.0	2.2896975744546683E-5	0.019233459625419214	3.205576604236536E-4
25	4.5793951489093366E-5	0.0	2.2896975744546683E-5	0.022439036229655752	3.205576604236536E-4
26	4.5793951489093366E-5	0.0	2.2896975744546683E-5	0.026537594887929606	3.6635161191274693E-4
27	4.5793951489093366E-5	0.0	2.2896975744546683E-5	0.046572448664407955	3.6635161191274693E-4
28	4.5793951489093366E-5	0.0	2.2896975744546683E-5	0.18645007348784365	3.6635161191274693E-4
29	4.5793951489093366E-5	0.0	2.2896975744546683E-5	0.44001118288295366	3.6635161191274693E-4
30	6.869092723364006E-5	0.0	2.2896975744546683E-5	0.7362980490173877	3.6635161191274693E-4
31	6.869092723364006E-5	0.0	2.2896975744546683E-5	1.467215308734807	3.6635161191274693E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	7420	0.0	17.128706	37
AACGCAG	21925	0.0	16.158495	5
TATACAC	2665	0.0	15.89681	37
TATACCG	220	1.8189894E-11	14.295454	5
GTACTAG	325	0.0	14.23077	1
ACGCAGA	24965	0.0	14.183456	6
CGCAGAG	25055	0.0	14.147276	7
GGACCGT	290	0.0	14.034483	6
AGAGTAC	25830	0.0	13.629694	10
CAGAGTA	26350	0.0	13.451992	9
GCAGAGT	26675	0.0	13.322774	8
TAGACAG	670	0.0	12.977612	5
TAGAGAG	1255	0.0	12.972113	5
GGGTAAG	1130	0.0	12.933627	1
GAGTACT	18010	0.0	12.881178	11
ATACCGA	275	3.6379788E-12	12.781818	6
GTCTTAG	745	0.0	12.66443	1
TATTCCG	190	1.0480835E-7	12.657895	5
GTATAAG	645	0.0	12.620155	1
GTATTAA	865	0.0	12.618498	1
>>END_MODULE