FastQCFastQC Report
Thu 9 Feb 2017
SRR2727326.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727326.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2798855
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT508301.8160997979530917No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT186070.6648075730968557No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT165030.5896339753220513No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124900.44625391454719876No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA58110.20762061628773193No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33060.1181197311043266No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31930.11408236582459612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG171050.017.7591345
CTTATAC30250.017.4909137
TAGACGG700.002593740115.8571435
AACCGTC1659.749783E-1015.696977
TATACAC11250.015.62222337
AGTCGGT3650.015.2054811
ACGCAGA202650.014.9716266
CGCAGAG203300.014.905567
GGAGTGT10350.014.6570056
GAGTGTC8600.014.6279077
GGCGTCA4050.014.61728412
AGAGTAC210550.014.19021610
TATACAG5250.014.0952385
CAGAGTA213000.014.0791079
GCAGAGT213750.014.0643288
TCGTCGA800.00630160113.87531
CGTAAAC800.00630160113.8753
TATATCG950.001245855113.6315795
TATCTCG950.001245855113.6315795
GACCGTG4250.013.4941187
Produced by FastQC (version 0.11.5)