##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727326.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2798855
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.192060681957443	31.0	31.0	34.0	30.0	34.0
2	31.346910790305323	31.0	31.0	34.0	30.0	34.0
3	31.387258361008342	31.0	31.0	34.0	28.0	34.0
4	35.21494754104803	37.0	35.0	37.0	32.0	37.0
5	34.41233432957406	35.0	35.0	37.0	30.0	37.0
6	34.41312536733771	35.0	35.0	37.0	30.0	37.0
7	34.39807492706839	35.0	35.0	37.0	30.0	37.0
8	34.28633959244048	35.0	35.0	37.0	30.0	37.0
9	35.98760814690293	37.0	35.0	39.0	32.0	39.0
10	35.55234980018615	37.0	35.0	39.0	30.0	39.0
11	35.800617752616695	37.0	35.0	39.0	30.0	39.0
12	35.63408215145122	37.0	35.0	39.0	30.0	39.0
13	35.82093070201922	37.0	35.0	39.0	30.0	39.0
14	36.566975066589734	38.0	36.0	40.0	31.0	41.0
15	36.44625713014786	38.0	35.0	40.0	31.0	41.0
16	36.39493292792946	38.0	35.0	40.0	30.0	41.0
17	36.29992264693955	38.0	35.0	40.0	30.0	41.0
18	36.25446763051319	38.0	35.0	40.0	30.0	41.0
19	36.31690137574115	38.0	35.0	40.0	30.0	41.0
20	36.24772380134019	38.0	35.0	40.0	30.0	41.0
21	36.18545833921372	38.0	35.0	40.0	30.0	41.0
22	36.12687438256001	38.0	34.0	40.0	30.0	41.0
23	36.012835605988876	38.0	34.0	40.0	29.0	41.0
24	35.90735640109973	38.0	34.0	40.0	29.0	41.0
25	35.74825562596133	38.0	34.0	40.0	29.0	41.0
26	35.5152428403758	38.0	34.0	40.0	27.0	41.0
27	35.3398539760009	38.0	34.0	40.0	27.0	41.0
28	35.16819913857631	38.0	34.0	40.0	27.0	41.0
29	35.00208156549732	37.0	33.0	40.0	27.0	40.0
30	34.86110355841942	37.0	33.0	39.0	26.0	40.0
31	34.66776092366343	37.0	33.0	39.0	25.0	40.0
32	34.502952814633126	37.0	33.0	39.0	25.0	40.0
33	34.32249116156428	37.0	33.0	39.0	25.0	40.0
34	34.14330538738162	37.0	33.0	39.0	24.0	40.0
35	34.01357269311915	37.0	33.0	39.0	24.0	40.0
36	33.84386615240875	36.0	32.0	39.0	24.0	40.0
37	33.677980817155586	36.0	32.0	39.0	23.0	40.0
38	33.49674563348226	36.0	31.0	39.0	22.0	40.0
39	33.2612382563584	36.0	31.0	39.0	21.0	40.0
40	33.05764142836981	36.0	31.0	39.0	21.0	40.0
41	32.84989540365614	36.0	31.0	39.0	19.0	40.0
42	32.6554358836024	36.0	30.0	39.0	18.0	40.0
43	31.03118632440766	34.0	28.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	1.0
11	2.0
12	4.0
13	17.0
14	24.0
15	64.0
16	108.0
17	270.0
18	539.0
19	1160.0
20	2219.0
21	4161.0
22	6990.0
23	11046.0
24	17053.0
25	25320.0
26	36198.0
27	49782.0
28	66539.0
29	86574.0
30	109808.0
31	137828.0
32	171430.0
33	214569.0
34	271908.0
35	342257.0
36	412511.0
37	425044.0
38	318247.0
39	87179.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.601849684960456	19.190490396965902	14.264190177769123	23.94346974030452
2	17.370031673666553	23.966550607301915	34.76503784583338	23.89837987319815
3	19.378960324847124	23.679861943544772	31.86145763178157	25.079720099826535
4	14.20734550378637	16.096403707944855	34.25111340173035	35.44513738653842
5	14.333932983309246	38.755491084747156	33.42841983596864	13.482156095974961
6	34.10984134583607	36.53622642116151	14.634520187719621	14.719412045282803
7	28.187312311641726	31.939239438984874	21.317181490287993	18.556266759085414
8	28.688695913150198	35.610276345148286	19.029603177013456	16.67142456468806
9	26.63206918543476	13.624214187587425	19.21993100750128	40.52378561947654
10	15.003063752856077	29.521679401040785	33.469257964417594	22.005998881685546
11	36.02676808909357	22.242988650716097	21.526910111456292	20.203333148734036
12	20.66366424841587	26.951056771429744	31.094822704284432	21.290456275869953
13	32.0813689883899	19.51930342943811	25.04988647143207	23.349441110739928
14	21.153507416425647	20.810259909855993	28.931580950067083	29.10465172365128
15	24.459752291562083	29.57502264318802	23.494821989706505	22.47040307554339
16	22.321949511496666	26.974816487456476	26.006241838180255	24.6969921628666
17	22.749910231148093	27.196121271019756	27.647055670979743	22.40691282685241
18	22.365681680544366	25.971156062032506	28.668902104610634	22.99426015281249
19	23.71509063527764	26.34034274730202	29.044591449003253	20.899975168417086
20	22.740906549285334	26.47014582749017	28.792059610090554	21.99688801313394
21	22.316161430299175	26.970779122176747	29.03158613075704	21.681473316767036
22	22.965855680269254	26.428343018841634	28.259734784402905	22.346066516486207
23	22.100001607800333	26.630532842894684	28.68748113067665	22.58198441862833
24	22.07227598428643	26.306543211420387	29.096398348610414	22.52478245568277
25	22.406841369059848	26.22247311847166	28.82228625634411	22.54839925612438
26	21.98463300170963	26.6733003317428	29.315345025019155	22.02672164152841
27	22.505381665002293	27.066854124275817	28.792131067883115	21.635633142838767
28	22.02397051651479	27.608397005203916	28.442988293427135	21.924644184854163
29	21.835536317529847	27.71704857879383	28.830325258007296	21.61708984566903
30	22.24920547866896	27.563485782578944	28.425874152108634	21.761434586643468
31	21.852936290018597	27.162607566308367	28.816533904042902	22.167922239630133
32	21.473852700479306	27.568737930332226	28.61920320988404	22.33820615930443
33	21.76786578797401	26.977460425781256	28.652002336669817	22.602671449574917
34	21.942687277475965	27.015761802594273	29.197046649433428	21.84450427049633
35	21.960408810031247	26.888102456182978	28.780304803214175	22.371183930571608
36	21.602226624816222	26.581155508234616	29.285868685587502	22.530749181361664
37	21.266410728672973	26.459998820946424	29.415385934605403	22.858204515775203
38	21.386995753620678	26.24012319323438	29.5752370165657	22.797644036579243
39	20.83955760480625	26.346130828499508	30.24933410269557	22.56497746399867
40	21.21974879013025	26.120217017316012	30.23068361883699	22.429350573716754
41	20.657197318189045	26.022319841506615	30.576753708212824	22.743729132091516
42	20.219375423164117	26.2867851317771	30.34930355448925	23.144535890569536
43	20.01379135396439	25.731415167988338	30.143898129770925	24.110895348276348
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1483.0
1	1462.5
2	1442.0
3	3315.5
4	5189.0
5	5189.0
6	7179.5
7	9170.0
8	9275.5
9	9381.0
10	12538.0
11	15695.0
12	15695.0
13	26356.5
14	37018.0
15	62664.0
16	88310.0
17	68522.0
18	48734.0
19	48734.0
20	51175.5
21	53617.0
22	41312.0
23	29007.0
24	32659.5
25	36312.0
26	36312.0
27	42219.0
28	48126.0
29	52661.5
30	57197.0
31	63357.0
32	69517.0
33	69517.0
34	76835.5
35	84154.0
36	94469.5
37	104785.0
38	116212.5
39	127640.0
40	127640.0
41	140410.5
42	153181.0
43	165752.0
44	178323.0
45	190231.5
46	202140.0
47	202140.0
48	214474.0
49	226808.0
50	230166.5
51	233525.0
52	226237.0
53	218949.0
54	218949.0
55	204022.5
56	189096.0
57	174325.5
58	159555.0
59	146318.0
60	133081.0
61	133081.0
62	115319.0
63	97557.0
64	81563.0
65	65569.0
66	54178.5
67	42788.0
68	42788.0
69	34950.5
70	27113.0
71	22252.0
72	17391.0
73	14164.5
74	10938.0
75	10938.0
76	8872.5
77	6807.0
78	5492.0
79	4177.0
80	3273.5
81	2370.0
82	2370.0
83	1872.5
84	1375.0
85	1046.5
86	718.0
87	570.5
88	423.0
89	423.0
90	275.5
91	128.0
92	82.5
93	37.0
94	26.0
95	15.0
96	15.0
97	13.0
98	11.0
99	7.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2798855.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.835450034039404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.481993985103	51.3950012385675
2	8.428236182192784	10.254710822737442
3	2.596984262110867	4.73966118950497
4	1.2534835061595666	3.0502493282985133
5	0.7397914955057189	2.2502774280222724
6	0.4818191308447887	1.7587010195971462
7	0.3586935566680788	1.5274898760948952
8	0.2672344145761873	1.3005860700260332
9	0.19646137336605674	1.0756634456726553
>10	1.1111202430915192	12.105844166813982
>50	0.056577266594582405	2.328628405338521
>100	0.024436244696054283	2.658530076110236
>500	0.0019362072221730832	0.8082895164117057
>1k	9.387671380033268E-4	1.0255942890116176
>5k	5.867294612520793E-5	0.2074175482353569
>10k+	2.346917845008317E-4	3.5133555795570786
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	50830	1.8160997979530917	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18607	0.6648075730968557	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16503	0.5896339753220513	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12490	0.44625391454719876	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	5811	0.20762061628773193	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3306	0.1181197311043266	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3193	0.11408236582459612	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.572889628080054E-5	0.0
5	0.0	0.0	0.0	3.572889628080054E-5	0.0
6	0.0	0.0	0.0	3.572889628080054E-5	3.572889628080054E-5
7	0.0	0.0	0.0	3.572889628080054E-5	3.572889628080054E-5
8	0.0	0.0	0.0	3.572889628080054E-5	3.572889628080054E-5
9	0.0	0.0	0.0	7.145779256160108E-5	7.145779256160108E-5
10	0.0	0.0	0.0	7.145779256160108E-5	7.145779256160108E-5
11	7.145779256160108E-5	0.0	0.0	1.0718668884240163E-4	7.145779256160108E-5
12	7.145779256160108E-5	0.0	0.0	1.786444814040027E-4	1.0718668884240163E-4
13	7.145779256160108E-5	3.572889628080054E-5	0.0	1.786444814040027E-4	1.0718668884240163E-4
14	1.0718668884240163E-4	3.572889628080054E-5	0.0	2.5010227396560377E-4	1.0718668884240163E-4
15	1.0718668884240163E-4	3.572889628080054E-5	0.0	4.6447565165040703E-4	1.4291558512320217E-4
16	1.0718668884240163E-4	3.572889628080054E-5	0.0	7.503068218968114E-4	1.4291558512320217E-4
17	1.0718668884240163E-4	3.572889628080054E-5	0.0	0.0011433246809856173	1.4291558512320217E-4
18	1.0718668884240163E-4	3.572889628080054E-5	0.0	0.001250511369828019	1.786444814040027E-4
19	2.1437337768480326E-4	3.572889628080054E-5	0.0	0.0018221737103208276	1.786444814040027E-4
20	2.1437337768480326E-4	3.572889628080054E-5	0.0	0.0025367516359368386	1.786444814040027E-4
21	2.1437337768480326E-4	3.572889628080054E-5	0.0	0.00396590748716886	1.786444814040027E-4
22	2.5010227396560377E-4	3.572889628080054E-5	0.0	0.006502659123105698	1.786444814040027E-4
23	2.5010227396560377E-4	3.572889628080054E-5	0.0	0.0075745260115297145	3.215600665272049E-4
24	2.5010227396560377E-4	3.572889628080054E-5	0.0	0.010718668884240163	3.215600665272049E-4
25	2.5010227396560377E-4	3.572889628080054E-5	0.0	0.012076366942910583	3.215600665272049E-4
26	2.5010227396560377E-4	3.572889628080054E-5	0.0	0.01450593189000502	3.572889628080054E-4
27	2.5010227396560377E-4	3.572889628080054E-5	0.0	0.02436710726350597	3.9301785908880596E-4
28	2.5010227396560377E-4	3.572889628080054E-5	0.0	0.08814318712473494	3.9301785908880596E-4
29	2.5010227396560377E-4	3.572889628080054E-5	0.0	0.2378115336450084	3.9301785908880596E-4
30	2.5010227396560377E-4	3.572889628080054E-5	0.0	0.42124368715063837	3.9301785908880596E-4
31	2.5010227396560377E-4	3.572889628080054E-5	3.572889628080054E-5	0.9147312025810554	3.9301785908880596E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCAG	17105	0.0	17.759134	5
CTTATAC	3025	0.0	17.49091	37
TAGACGG	70	0.0025937401	15.857143	5
AACCGTC	165	9.749783E-10	15.69697	7
TATACAC	1125	0.0	15.622223	37
AGTCGGT	365	0.0	15.20548	11
ACGCAGA	20265	0.0	14.971626	6
CGCAGAG	20330	0.0	14.90556	7
GGAGTGT	1035	0.0	14.657005	6
GAGTGTC	860	0.0	14.627907	7
GGCGTCA	405	0.0	14.617284	12
AGAGTAC	21055	0.0	14.190216	10
TATACAG	525	0.0	14.095238	5
CAGAGTA	21300	0.0	14.079107	9
GCAGAGT	21375	0.0	14.064328	8
TCGTCGA	80	0.006301601	13.875	31
CGTAAAC	80	0.006301601	13.875	3
TATATCG	95	0.0012458551	13.631579	5
TATCTCG	95	0.0012458551	13.631579	5
GACCGTG	425	0.0	13.494118	7
>>END_MODULE