FastQCFastQC Report
Thu 9 Feb 2017
SRR2727325.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727325.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3568988
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT153070.42888908564556677No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT57130.160073387750253No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT52370.14673627369999565No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39690.11120799509552848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTTA5700.018.17543822
CTTATAC27150.017.7845337
TCGTTAC4800.017.3437523
CGTTACG3500.016.38571524
TATACCG2550.015.9607845
TAGTACT3950.015.4556964
TATACTG6650.015.3007525
TACGGAT5500.014.46363527
AACGCAG75900.014.4295125
ATCTCGT7450.014.15436220
TAAGACT5500.014.1272724
GTTACGG5200.013.87525
GATCTCG7800.013.75641119
TCTATAC4450.013.71913
TTATACT7700.013.6948054
GTATTAC3450.013.4057971
ATTATAC5250.013.3904773
GTACCGT2501.0913936E-1113.326
TACGGGT4050.013.2469144
CTAGTAC3800.013.1447373
Produced by FastQC (version 0.11.5)