##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727325.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3568988
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37398696773427	33.0	31.0	34.0	31.0	34.0
2	32.520915172592346	34.0	31.0	34.0	31.0	34.0
3	32.61520688777883	34.0	31.0	34.0	31.0	34.0
4	36.138792004904474	37.0	35.0	37.0	35.0	37.0
5	35.945137108894734	37.0	35.0	37.0	35.0	37.0
6	35.95516992491989	37.0	35.0	37.0	35.0	37.0
7	35.92054050055646	37.0	35.0	37.0	35.0	37.0
8	35.87361347250257	37.0	35.0	37.0	35.0	37.0
9	37.71474742980363	39.0	37.0	39.0	35.0	39.0
10	37.541298821962975	39.0	37.0	39.0	35.0	39.0
11	37.651791488231396	39.0	37.0	39.0	35.0	39.0
12	37.57425214094304	39.0	37.0	39.0	35.0	39.0
13	37.64881109154752	39.0	37.0	39.0	35.0	39.0
14	38.915885399446566	40.0	38.0	41.0	36.0	41.0
15	38.839606353397656	40.0	38.0	41.0	35.0	41.0
16	38.810405358605855	40.0	38.0	41.0	35.0	41.0
17	38.737614135995976	40.0	38.0	41.0	35.0	41.0
18	38.69820016206275	40.0	38.0	41.0	35.0	41.0
19	38.74970131589123	40.0	38.0	41.0	35.0	41.0
20	38.71531509772518	40.0	38.0	41.0	35.0	41.0
21	38.67678652884235	40.0	38.0	41.0	34.0	41.0
22	38.648795120633636	40.0	38.0	41.0	34.0	41.0
23	38.58736398105009	40.0	38.0	41.0	34.0	41.0
24	38.55062975835167	40.0	38.0	41.0	34.0	41.0
25	38.43774313614952	40.0	38.0	41.0	34.0	41.0
26	38.33999161667117	40.0	38.0	41.0	34.0	41.0
27	38.2616262649244	40.0	38.0	41.0	34.0	41.0
28	38.18262347757964	40.0	38.0	41.0	34.0	41.0
29	38.113030640618575	40.0	38.0	41.0	33.0	41.0
30	38.0630985590313	40.0	38.0	41.0	33.0	41.0
31	37.98676767756014	40.0	37.0	41.0	33.0	41.0
32	37.90896467009696	40.0	37.0	41.0	33.0	41.0
33	37.83234967447355	40.0	37.0	41.0	33.0	41.0
34	37.756220250670495	40.0	37.0	41.0	33.0	41.0
35	37.704566952872916	40.0	37.0	41.0	33.0	41.0
36	37.62943248898567	40.0	37.0	41.0	33.0	41.0
37	37.55919717298013	40.0	37.0	41.0	33.0	41.0
38	37.48102655430615	40.0	37.0	41.0	32.0	41.0
39	37.38734089327283	40.0	37.0	41.0	32.0	41.0
40	37.2858698320084	40.0	37.0	41.0	31.0	41.0
41	37.197671440755755	39.0	36.0	41.0	31.0	41.0
42	37.10454812400602	39.0	36.0	41.0	31.0	41.0
43	35.41177106787694	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	3.0
12	6.0
13	3.0
14	2.0
15	6.0
16	9.0
17	26.0
18	55.0
19	140.0
20	319.0
21	643.0
22	1276.0
23	2292.0
24	4054.0
25	6652.0
26	10161.0
27	15518.0
28	23239.0
29	32278.0
30	44464.0
31	59224.0
32	77831.0
33	103371.0
34	140262.0
35	196209.0
36	291088.0
37	486772.0
38	989480.0
39	1083601.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.28493455287605	17.233176463468077	11.698890553848878	26.78299842980699
2	16.760409393363048	19.73422157765731	36.98642304205001	26.518945986929626
3	18.26652261089138	21.837114610640327	30.788167402075885	29.10819537639241
4	13.256755136189865	14.727872438909854	35.70359440827484	36.31177801662544
5	14.072980912236185	37.22413748659284	35.12951010202332	13.573371499147658
6	35.78011469918083	36.093564898509044	14.697807894002446	13.428512508307676
7	30.190014648410134	30.9895970510408	20.791580134200508	18.028808166348558
8	27.891800140544042	36.34335559547973	18.703032904565664	17.061811359410566
9	27.12368324017901	13.177881236922062	17.872881612378634	41.82555391052029
10	14.55348126695859	27.579947032604203	34.87795419878128	22.98861750165593
11	37.83946597747037	20.56299993163328	21.577265039837624	20.02026905105873
12	21.125456291811574	24.386324638805174	31.642695352295945	22.84552371708731
13	31.667296163506293	18.896252943411408	24.536507267606392	24.899943625475906
14	21.83683441916868	18.793983056261325	26.68815361665548	32.68102890791451
15	25.28442236286589	27.786420128058708	22.013915429247728	24.91524207982767
16	24.876435561004968	26.059684145757846	24.228941089182705	24.834939204054482
17	24.215211707072147	26.29818312642127	25.59493055174184	23.891674614764746
18	24.179431256143197	25.825948420112365	26.006475785292636	23.9881445384518
19	25.163855972617448	25.610985523067043	25.868229313183456	23.356929191132053
20	24.56186459579018	25.410256352781236	25.752230043922815	24.27564900750577
21	24.226979748881195	25.63328876420991	26.03782921096961	24.101902275939285
22	24.80924564610472	24.98313247340703	25.502439346952134	24.705182533536117
23	24.20352772270459	25.108882405880884	25.89717309220429	24.790416779210243
24	24.187865019439684	25.018744809452986	25.989103914050705	24.804286257056624
25	24.62742940015489	24.919024664694867	25.78630132687473	24.66724460827551
26	24.32047964296882	25.130709321521955	26.029927811469243	24.518883224039982
27	24.696580655356644	25.50215915548049	25.636146717220683	24.16511347194219
28	24.530539189260374	25.67086244055738	25.45318168623711	24.34541668394514
29	24.293217012777852	25.75536818840523	25.763605817671564	24.187808981145356
30	24.583635473136923	25.6032242193025	25.583106471638462	24.230033835922114
31	24.400894595330666	25.09966410646379	25.86010376050578	24.639337537699763
32	23.901621412008108	25.612078269806453	25.849064216523004	24.63723610166243
33	24.155446866170465	25.09638586624556	25.861224526392355	24.88694274119162
34	24.41599691565228	25.094312449355392	26.325753967231048	24.16393666776128
35	24.336618671735515	25.167414404307326	25.9912333692352	24.504733554721955
36	24.120394913067795	24.863294580984864	26.309839091641663	24.706471414305682
37	23.946424028323996	24.59660833827404	26.45256862729715	25.004399006104812
38	23.968923403497016	24.50882435020796	26.597651771314446	24.924600474980583
39	23.54563814728433	24.395711053105252	27.222786963699512	24.835863835910907
40	23.99901596755159	24.178590681728267	27.286054197996744	24.536339152723404
41	23.515041238580796	24.05513831932189	27.578882305011952	24.85093813708536
42	23.010584513032825	24.29187209371396	27.638535069324977	25.05900832392824
43	22.889990103637224	23.739250454190376	27.608526562711894	25.762232879460505
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	68.0
1	141.5
2	215.0
3	1181.0
4	2147.0
5	2147.0
6	3035.0
7	3923.0
8	3573.5
9	3224.0
10	4119.5
11	5015.0
12	5015.0
13	8331.0
14	11647.0
15	19174.5
16	26702.0
17	21295.0
18	15888.0
19	15888.0
20	17866.5
21	19845.0
22	18522.5
23	17200.0
24	21692.5
25	26185.0
26	26185.0
27	33113.0
28	40041.0
29	46834.5
30	53628.0
31	64078.5
32	74529.0
33	74529.0
34	87335.0
35	100141.0
36	116024.5
37	131908.0
38	150810.5
39	169713.0
40	169713.0
41	188134.5
42	206556.0
43	228035.0
44	249514.0
45	268732.5
46	287951.0
47	287951.0
48	304915.0
49	321879.0
50	327733.5
51	333588.0
52	329428.0
53	325268.0
54	325268.0
55	306925.5
56	288583.0
57	266174.0
58	243765.0
59	222777.5
60	201790.0
61	201790.0
62	174590.5
63	147391.0
64	123638.0
65	99885.0
66	82153.5
67	64422.0
68	64422.0
69	52319.0
70	40216.0
71	32266.5
72	24317.0
73	19333.0
74	14349.0
75	14349.0
76	11255.0
77	8161.0
78	6382.0
79	4603.0
80	3487.5
81	2372.0
82	2372.0
83	1818.0
84	1264.0
85	971.0
86	678.0
87	476.0
88	274.0
89	274.0
90	178.5
91	83.0
92	58.5
93	34.0
94	24.0
95	14.0
96	14.0
97	11.0
98	8.0
99	6.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3568988.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.33733647229732
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.50588054371049	45.58655424261005
2	10.901952423449037	12.501778286165491
3	3.645066072472743	6.269951395833748
4	1.7548781167599503	4.024801481941469
5	0.9867863109317208	2.828984936807454
6	0.643979619249638	2.215444566613104
7	0.4403966361128129	1.7675819074247763
8	0.33345681419801787	1.5295620443721663
9	0.24228517892219006	1.2502788143501133
>10	1.4344503816179632	14.963143617184446
>50	0.08221514733426749	3.207295019509557
>100	0.027578014382822237	2.563542464999053
>500	6.350741493472316E-4	0.24668718640295817
>1k	2.9311113938176826E-4	0.3089261873271209
>5k	9.770371312725608E-5	0.3067133694108236
>10k+	4.885185656362804E-5	0.42875447904762354
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15307	0.42888908564556677	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5713	0.160073387750253	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5237	0.14673627369999565	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3969	0.11120799509552848	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.603829432881254E-5
2	0.0	0.0	0.0	0.0	5.603829432881254E-5
3	0.0	0.0	0.0	0.0	5.603829432881254E-5
4	0.0	0.0	0.0	0.0	5.603829432881254E-5
5	0.0	0.0	0.0	0.0	5.603829432881254E-5
6	0.0	0.0	0.0	0.0	8.40574414932188E-5
7	0.0	0.0	0.0	2.801914716440627E-5	8.40574414932188E-5
8	0.0	0.0	0.0	2.801914716440627E-5	8.40574414932188E-5
9	0.0	0.0	0.0	8.40574414932188E-5	1.1207658865762507E-4
10	0.0	0.0	0.0	8.40574414932188E-5	1.1207658865762507E-4
11	0.0	0.0	0.0	1.4009573582203134E-4	1.1207658865762507E-4
12	0.0	0.0	0.0	1.681148829864376E-4	1.681148829864376E-4
13	0.0	0.0	0.0	1.681148829864376E-4	1.961340301508439E-4
14	0.0	0.0	0.0	1.681148829864376E-4	1.961340301508439E-4
15	0.0	0.0	0.0	1.681148829864376E-4	1.961340301508439E-4
16	0.0	0.0	0.0	1.961340301508439E-4	3.0821061880846894E-4
17	0.0	0.0	0.0	3.362297659728752E-4	3.0821061880846894E-4
18	0.0	0.0	0.0	4.2028720746609404E-4	4.2028720746609404E-4
19	0.0	0.0	0.0	7.004786791101567E-4	4.2028720746609404E-4
20	0.0	0.0	0.0	8.966127092610006E-4	4.483063546305003E-4
21	0.0	0.0	0.0	0.0014289765053847197	4.483063546305003E-4
22	0.0	0.0	0.0	0.0024656849504677515	4.483063546305003E-4
23	0.0	0.0	0.0	0.0030540870409202833	6.164212376169379E-4
24	0.0	0.0	0.0	0.004062776338838909	6.164212376169379E-4
25	0.0	0.0	0.0	0.0052395805197439724	6.164212376169379E-4
26	0.0	0.0	0.0	0.006248269817662598	6.444403847813442E-4
27	0.0	0.0	0.0	0.011992194986365882	6.444403847813442E-4
28	0.0	0.0	0.0	0.058560017573609104	6.444403847813442E-4
29	0.0	0.0	0.0	0.15099518406898538	6.444403847813442E-4
30	0.0	0.0	0.0	0.2594012644480732	6.444403847813442E-4
31	0.0	0.0	0.0	0.6240704647928208	6.444403847813442E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTTA	570	0.0	18.175438	22
CTTATAC	2715	0.0	17.78453	37
TCGTTAC	480	0.0	17.34375	23
CGTTACG	350	0.0	16.385715	24
TATACCG	255	0.0	15.960784	5
TAGTACT	395	0.0	15.455696	4
TATACTG	665	0.0	15.300752	5
TACGGAT	550	0.0	14.463635	27
AACGCAG	7590	0.0	14.429512	5
ATCTCGT	745	0.0	14.154362	20
TAAGACT	550	0.0	14.127272	4
GTTACGG	520	0.0	13.875	25
GATCTCG	780	0.0	13.756411	19
TCTATAC	445	0.0	13.7191	3
TTATACT	770	0.0	13.694805	4
GTATTAC	345	0.0	13.405797	1
ATTATAC	525	0.0	13.390477	3
GTACCGT	250	1.0913936E-11	13.32	6
TACGGGT	405	0.0	13.246914	4
CTAGTAC	380	0.0	13.144737	3
>>END_MODULE