FastQCFastQC Report
Thu 9 Feb 2017
SRR2727324.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727324.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3068188
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT64140.20904846769493915No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGTA450.003827241520.5555579
CTTATAC19050.019.13123537
TACTTAC12200.018.19672231
GGTATCA17200.017.2093031
GTACTTA14050.016.85409430
AACGCAG36850.016.8181825
ACGTTTA904.449849E-516.44444526
AGTGTAC16800.016.07738127
CAACGCA39550.015.7168144
ACGCAGA40400.015.5693066
TCAACGC40300.015.5620343
ACTTACA9850.015.40101632
ATCAACG40650.015.3370242
TCGTTAC13950.015.11827923
ATGGTTG41950.015.0822432
GGGTAAG33450.014.9880421
CTCGTTA13950.014.85304622
CCGTAAC8350.014.84431113
CGTCAGA12500.014.814
CGTCGAA1252.9624607E-614.79999922
Produced by FastQC (version 0.11.5)