##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727324.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3068188
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34260970970488	33.0	31.0	34.0	31.0	34.0
2	32.497093398448854	34.0	31.0	34.0	31.0	34.0
3	32.58824296294751	34.0	31.0	34.0	31.0	34.0
4	36.123183129586586	37.0	35.0	37.0	35.0	37.0
5	35.924961247485484	37.0	35.0	37.0	35.0	37.0
6	35.93008902974655	37.0	35.0	37.0	35.0	37.0
7	35.899685416930126	37.0	35.0	37.0	35.0	37.0
8	35.84919633347109	37.0	35.0	37.0	35.0	37.0
9	37.697473883608176	39.0	37.0	39.0	35.0	39.0
10	37.50678054930141	39.0	37.0	39.0	35.0	39.0
11	37.621269296405565	39.0	37.0	39.0	35.0	39.0
12	37.543093513174554	39.0	37.0	39.0	35.0	39.0
13	37.6234542994106	39.0	37.0	39.0	35.0	39.0
14	38.88537240873114	40.0	38.0	41.0	36.0	41.0
15	38.81094313647012	40.0	38.0	41.0	35.0	41.0
16	38.767198750532884	40.0	38.0	41.0	35.0	41.0
17	38.70028694460704	40.0	38.0	41.0	35.0	41.0
18	38.64444942747967	40.0	38.0	41.0	34.0	41.0
19	38.7059150221564	40.0	38.0	41.0	34.0	41.0
20	38.66795776529991	40.0	38.0	41.0	34.0	41.0
21	38.63214868189303	40.0	38.0	41.0	34.0	41.0
22	38.611256220283764	40.0	38.0	41.0	34.0	41.0
23	38.5526154199156	40.0	38.0	41.0	34.0	41.0
24	38.521940311349894	40.0	38.0	41.0	34.0	41.0
25	38.40575088619081	40.0	38.0	41.0	34.0	41.0
26	38.31381356031638	40.0	38.0	41.0	34.0	41.0
27	38.2382393125845	40.0	38.0	41.0	34.0	41.0
28	38.14597247626286	40.0	38.0	41.0	34.0	41.0
29	38.08916109443098	40.0	38.0	41.0	33.0	41.0
30	38.060137123279276	40.0	38.0	41.0	33.0	41.0
31	37.97716795711345	40.0	38.0	41.0	33.0	41.0
32	37.90646629215681	40.0	37.0	41.0	33.0	41.0
33	37.842048792316504	40.0	37.0	41.0	33.0	41.0
34	37.77572365187531	40.0	37.0	41.0	33.0	41.0
35	37.74438170020872	40.0	37.0	41.0	33.0	41.0
36	37.65820738494512	40.0	37.0	41.0	33.0	41.0
37	37.61893208629979	40.0	37.0	41.0	33.0	41.0
38	37.5580639126416	40.0	37.0	41.0	32.0	41.0
39	37.44830108194152	40.0	37.0	41.0	32.0	41.0
40	37.38374115275857	40.0	37.0	41.0	32.0	41.0
41	37.29729794914783	39.0	37.0	41.0	31.0	41.0
42	37.2338898398664	39.0	37.0	41.0	31.0	41.0
43	35.52788421048515	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	2.0
12	3.0
13	1.0
14	5.0
15	9.0
16	19.0
17	21.0
18	59.0
19	107.0
20	241.0
21	549.0
22	1115.0
23	1964.0
24	3421.0
25	5587.0
26	8665.0
27	13043.0
28	18891.0
29	27072.0
30	37723.0
31	50527.0
32	67183.0
33	89386.0
34	122964.0
35	171181.0
36	252403.0
37	417802.0
38	864361.0
39	913881.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.836557603380236	16.99912130547411	11.614901042569752	27.5494200485759
2	17.031681239871872	19.280956707998335	37.36286694296438	26.324495109165408
3	18.005122241531485	21.462309350013754	31.072835171769135	29.459733236685626
4	13.556079353677152	14.64075864973072	35.675225898804115	36.12793609778801
5	13.836212122594835	37.6882055467266	35.14602103912798	13.329561291550581
6	35.85151887693974	35.96963419451481	14.873208551757585	13.305638376787861
7	30.359091424645424	31.61061186602646	20.413058130727322	17.617238578600787
8	27.71345823658785	37.173504361531954	18.440982104095315	16.672055297784883
9	28.39737982157547	12.870593327397147	17.299950328988967	41.432076522038415
10	14.426919080577852	27.592572554224187	34.8266794603199	23.153828904878058
11	38.425089987966835	20.47266986247257	21.231163149063878	19.871077000496708
12	21.09499157157254	23.83726160196181	31.465575121211607	23.602171705254047
13	31.567361582797403	18.971686220009985	24.170617967347503	25.290334229845108
14	21.705938488775786	18.711141559774042	26.26204782757771	33.32087212387246
15	25.99355059077214	27.546454128625758	21.506895926846724	24.953099353755377
16	24.763736772322947	26.38638831779539	24.065539660542314	24.78433524933935
17	24.47353291258554	25.942249953392686	24.797502630216922	24.786714503804852
18	24.24512448389734	26.58578939752062	25.429699874975064	23.739386243606976
19	26.018907576719545	25.449972426722223	25.06146950577996	23.46965049077827
20	24.657811059817718	24.928133478131066	25.24639950355063	25.16765595850059
21	24.401927130932002	25.38583033373444	26.165215430084466	24.047027105249093
22	25.261261695828285	24.239714124427838	24.868293598697342	25.630730581046535
23	24.28361625819539	24.890554294586902	25.959002512231976	24.86682693498573
24	24.014108653055157	24.30969027973514	26.134089566871392	25.54211150033831
25	24.862850646700917	24.42894633575257	25.36308074994101	25.345122267605507
26	24.445633709538008	24.5974496999532	25.980285432313792	24.976631158195
27	25.184864812716825	25.340494128782197	25.01851255529322	24.456128503207754
28	24.435888543987527	26.163129508361287	24.30613769430035	25.09484425335084
29	24.565215690824683	25.87765156502796	25.2394898878426	24.317642856304765
30	25.939121070807918	25.114790879828746	24.4593551633733	24.486732885990037
31	25.002314069411653	24.92523274323477	25.43599023267153	24.63646295468205
32	24.40740267545535	25.501207879047826	25.137736018783723	24.953653426713096
33	24.423861901552314	24.493218798848048	25.434882086756094	25.648037212843544
34	24.86715286025498	24.877973579193974	26.212572371706038	24.042301188845013
35	24.91092462391483	24.63766887817826	25.35278151143281	25.0986249864741
36	24.530928352499913	24.399352321304953	26.212148668856017	24.85757065733912
37	24.153441705658192	24.468904773762233	26.43416896226698	24.943484558312594
38	25.12538345107927	23.900425919141853	25.898282634571284	25.075907995207597
39	23.67384267196143	24.305388066181084	27.171737846572636	24.849031415284852
40	25.100254612820333	23.39618693509003	26.814393381370373	24.689165070719266
41	23.89201704719528	24.027015293717334	26.955714578115813	25.12525308097157
42	23.682544876650322	23.98441686102677	27.222223670778973	25.110814591543935
43	22.997124035424164	23.55680290777488	26.803344514742903	26.642728542058048
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	68.0
1	104.0
2	140.0
3	513.5
4	887.0
5	887.0
6	1160.0
7	1433.0
8	1289.5
9	1146.0
10	1619.0
11	2092.0
12	2092.0
13	3366.5
14	4641.0
15	7835.0
16	11029.0
17	9563.0
18	8097.0
19	8097.0
20	9823.5
21	11550.0
22	12391.5
23	13233.0
24	17238.5
25	21244.0
26	21244.0
27	26845.0
28	32446.0
29	38470.0
30	44494.0
31	53249.5
32	62005.0
33	62005.0
34	72949.0
35	83893.0
36	97447.5
37	111002.0
38	126814.5
39	142627.0
40	142627.0
41	157738.5
42	172850.0
43	191696.5
44	210543.0
45	232668.0
46	254793.0
47	254793.0
48	273414.0
49	292035.0
50	300392.5
51	308750.0
52	300499.0
53	292248.0
54	292248.0
55	271227.0
56	250206.0
57	229958.5
58	209711.0
59	192584.5
60	175458.0
61	175458.0
62	151266.0
63	127074.0
64	105803.5
65	84533.0
66	69572.5
67	54612.0
68	54612.0
69	44182.5
70	33753.0
71	27340.5
72	20928.0
73	16858.5
74	12789.0
75	12789.0
76	9965.0
77	7141.0
78	5671.0
79	4201.0
80	3233.0
81	2265.0
82	2265.0
83	1724.5
84	1184.0
85	905.5
86	627.0
87	445.5
88	264.0
89	264.0
90	192.5
91	121.0
92	78.0
93	35.0
94	31.0
95	27.0
96	27.0
97	18.5
98	10.0
99	6.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3068188.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.2908549069526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.74525033905269	44.88928316354228
2	10.601172822622647	11.934981624035613
3	3.5589163981097958	6.010033397759187
4	1.7483944001556748	3.936744619971662
5	0.9836535476360141	2.7685349564344035
6	0.6603291369724514	2.2302294984089697
7	0.4602678824369315	1.8136210812011344
8	0.3338121311249991	1.5032456191470371
9	0.26888736257602047	1.3622309561771933
>10	1.5152720095255086	15.653473536341552
>50	0.08787241274619745	3.362177103026012
>100	0.03408767141005018	3.2312227652967103
>500	0.0014471427995269039	0.5395644526684458
>1k	5.788571194905708E-4	0.5556611273193345
>5k	5.7885711949057074E-5	0.208996098670393
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6414	0.20904846769493915	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.259252692468649E-5
2	0.0	0.0	0.0	0.0	3.259252692468649E-5
3	0.0	0.0	0.0	0.0	3.259252692468649E-5
4	0.0	0.0	0.0	0.0	3.259252692468649E-5
5	0.0	0.0	0.0	3.259252692468649E-5	3.259252692468649E-5
6	0.0	0.0	0.0	3.259252692468649E-5	6.518505384937298E-5
7	0.0	0.0	0.0	3.259252692468649E-5	6.518505384937298E-5
8	6.518505384937298E-5	0.0	0.0	3.259252692468649E-5	6.518505384937298E-5
9	6.518505384937298E-5	0.0	0.0	1.3037010769874596E-4	1.3037010769874596E-4
10	1.3037010769874596E-4	0.0	0.0	1.3037010769874596E-4	1.3037010769874596E-4
11	1.3037010769874596E-4	0.0	0.0	1.3037010769874596E-4	1.6296263462343248E-4
12	1.3037010769874596E-4	0.0	0.0	1.9555516154811896E-4	2.2814768847280545E-4
13	1.3037010769874596E-4	0.0	0.0	2.2814768847280545E-4	2.2814768847280545E-4
14	1.3037010769874596E-4	0.0	0.0	2.2814768847280545E-4	2.607402153974919E-4
15	1.3037010769874596E-4	0.0	0.0	2.607402153974919E-4	4.237028500209244E-4
16	1.6296263462343248E-4	0.0	0.0	2.607402153974919E-4	4.562953769456109E-4
17	1.6296263462343248E-4	0.0	0.0	3.9111032309623793E-4	4.562953769456109E-4
18	1.6296263462343248E-4	0.0	0.0	4.237028500209244E-4	4.562953769456109E-4
19	1.6296263462343248E-4	0.0	0.0	4.562953769456109E-4	4.888879038702974E-4
20	1.6296263462343248E-4	0.0	0.0	5.866654846443569E-4	4.888879038702974E-4
21	1.6296263462343248E-4	0.0	0.0	7.170355923431028E-4	4.888879038702974E-4
22	1.6296263462343248E-4	0.0	0.0	0.0012711085500627733	4.888879038702974E-4
23	1.6296263462343248E-4	0.0	0.0	0.0013688861308368326	5.866654846443569E-4
24	1.6296263462343248E-4	0.0	0.0	0.0016948114000836976	5.866654846443569E-4
25	1.6296263462343248E-4	0.0	0.0	0.0018577740347071302	5.866654846443569E-4
26	1.6296263462343248E-4	0.0	0.0	0.002444439519351487	5.866654846443569E-4
27	1.6296263462343248E-4	0.0	0.0	0.006909615708033536	6.192580115690434E-4
28	1.6296263462343248E-4	0.0	0.0	0.03744881343646478	6.518505384937299E-4
29	1.6296263462343248E-4	0.0	0.0	0.09983090997031473	6.518505384937299E-4
30	1.6296263462343248E-4	0.0	0.0	0.17140409909692628	6.518505384937299E-4
31	1.6296263462343248E-4	0.0	0.0	0.5063249057750047	6.844430654184163E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCGTA	45	0.0038272415	20.555557	9
CTTATAC	1905	0.0	19.131235	37
TACTTAC	1220	0.0	18.196722	31
GGTATCA	1720	0.0	17.209303	1
GTACTTA	1405	0.0	16.854094	30
AACGCAG	3685	0.0	16.818182	5
ACGTTTA	90	4.449849E-5	16.444445	26
AGTGTAC	1680	0.0	16.077381	27
CAACGCA	3955	0.0	15.716814	4
ACGCAGA	4040	0.0	15.569306	6
TCAACGC	4030	0.0	15.562034	3
ACTTACA	985	0.0	15.401016	32
ATCAACG	4065	0.0	15.337024	2
TCGTTAC	1395	0.0	15.118279	23
ATGGTTG	4195	0.0	15.08224	32
GGGTAAG	3345	0.0	14.988042	1
CTCGTTA	1395	0.0	14.853046	22
CCGTAAC	835	0.0	14.844311	13
CGTCAGA	1250	0.0	14.8	14
CGTCGAA	125	2.9624607E-6	14.799999	22
>>END_MODULE