FastQCFastQC Report
Thu 9 Feb 2017
SRR2727322.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727322.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3118433
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT319211.0236230824904686No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT121300.38897741269413194No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT111010.3559800707598977No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86580.2776394426303211No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39130.1254796880356256No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA36660.1175590432759017No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34180.10960633112848665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACCG1205.18412E-918.55
ATACCGT1750.017.9714286
CTTATAC35500.017.61408437
TAATACC3900.016.6025644
AACGCAG121350.015.2756485
TCGTTAC5050.014.65346423
CGTTACG3900.014.2307724
TATACTG4950.014.2020215
CTAGGAC4700.014.1702123
GTATACG1702.4412657E-814.1470591
TACCGTC1458.923289E-714.0344837
TATACCG1854.9076334E-914.05
ATACCGA1854.9076334E-914.06
GTCTTAG5450.013.9174311
GTTACGG5600.013.87500125
CTCGTTA4950.013.82828322
GGAGTGT10000.013.696
ACGCAGA138150.013.5921116
GTTCTAG3700.013.51
CGCAGAG140050.013.3548737
Produced by FastQC (version 0.11.5)