FastQCFastQC Report
Thu 9 Feb 2017
SRR2727321.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727321.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258458
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT143891.143383410491252No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46020.36568562478843153No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37800.30036759272061525No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31020.24649213561358424No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA15650.12435854037242403No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT13500.10727414025736258No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA303.5996715E-430.83333210
GTATTAG706.5938366E-926.428571
ATACTAG705.10256E-621.1428571
TATCTCG450.00382600620.5555575
GTCGCGT555.1434664E-420.1818188
ACGGTAG656.902797E-519.92307719
GTATTAT1052.2599124E-819.380951
AACGGTA701.2193143E-418.518
TAGTACG500.007035243318.54
TATAGTG1552.0008883E-1117.9032255
ACCGTCG953.606936E-617.5263168
CTTATAC11050.016.74208337
ATAATAC1151.2423479E-616.0869563
AACGCAG65850.015.2551265
GTAATAC1001.0934945E-414.83
GTAGGAC1252.959101E-614.7999993
TAATACT1152.210063E-514.4782614
TGAACCG1152.210063E-514.4782615
CGGTAGT908.274781E-414.38888920
CTAGAGT1702.4365363E-814.14705854
Produced by FastQC (version 0.11.5)