##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727321.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1258458
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.41611639005831	31.0	30.0	31.0	27.0	34.0
2	30.564409777680304	31.0	30.0	31.0	27.0	34.0
3	30.613965662739638	31.0	30.0	33.0	27.0	34.0
4	34.41148453106897	35.0	35.0	37.0	30.0	37.0
5	32.870268217135575	35.0	32.0	37.0	26.0	37.0
6	32.95247596661947	35.0	32.0	37.0	26.0	37.0
7	32.98749660298556	35.0	32.0	36.0	26.0	37.0
8	32.81276609946458	35.0	32.0	36.0	26.0	37.0
9	34.357861764159	37.0	33.0	39.0	28.0	39.0
10	33.69715556657433	35.0	32.0	38.0	27.0	39.0
11	34.10331055943067	35.0	32.0	38.0	27.0	39.0
12	33.830796895883694	35.0	32.0	38.0	27.0	39.0
13	34.106453294428576	35.0	32.0	38.0	27.0	39.0
14	34.61569873607224	37.0	33.0	39.0	27.0	40.0
15	34.54867623710923	37.0	32.0	39.0	27.0	40.0
16	34.4536742584973	37.0	32.0	39.0	27.0	40.0
17	34.297189099675954	36.0	32.0	39.0	26.0	40.0
18	34.20984728930167	36.0	32.0	39.0	26.0	40.0
19	34.25950250226865	37.0	32.0	39.0	25.0	40.0
20	34.17221711014591	36.0	32.0	39.0	25.0	40.0
21	34.07979845175604	36.0	32.0	39.0	25.0	40.0
22	34.02009284378183	36.0	32.0	39.0	25.0	40.0
23	33.87223014196739	36.0	32.0	39.0	25.0	40.0
24	33.72993695459046	36.0	31.0	39.0	24.0	40.0
25	33.53867431412093	36.0	31.0	39.0	24.0	40.0
26	33.23591808387725	36.0	31.0	38.0	23.0	40.0
27	33.01935066565591	36.0	31.0	38.0	23.0	40.0
28	32.82210292278328	36.0	30.0	38.0	21.0	40.0
29	32.62535181944888	35.0	30.0	38.0	21.0	40.0
30	32.46399800390637	35.0	30.0	38.0	19.0	40.0
31	32.27037215385813	35.0	30.0	38.0	19.0	40.0
32	32.086938141757614	35.0	30.0	38.0	19.0	40.0
33	31.926302665643192	35.0	30.0	38.0	18.0	40.0
34	31.708842090876296	35.0	29.0	38.0	18.0	40.0
35	31.553389147671197	34.0	29.0	38.0	17.0	40.0
36	31.384012021060695	34.0	28.0	38.0	16.0	40.0
37	31.210567217976287	34.0	28.0	38.0	16.0	40.0
38	30.98367923283892	34.0	27.0	38.0	15.0	40.0
39	30.763352451969	34.0	27.0	38.0	15.0	40.0
40	30.519875911631537	34.0	26.0	38.0	15.0	40.0
41	30.32990930170097	33.0	26.0	38.0	14.0	39.0
42	30.129348774452545	33.0	26.0	38.0	13.0	39.0
43	28.493453098951257	32.0	24.0	37.0	9.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	1.0
12	13.0
13	29.0
14	51.0
15	143.0
16	271.0
17	547.0
18	1078.0
19	1968.0
20	3391.0
21	5630.0
22	8914.0
23	13376.0
24	19598.0
25	26910.0
26	36194.0
27	47170.0
28	59118.0
29	72794.0
30	86847.0
31	100273.0
32	113082.0
33	123993.0
34	131227.0
35	129088.0
36	115481.0
37	87710.0
38	55862.0
39	17691.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.14355981685523	19.62195003726783	13.542843702372268	24.69164644350467
2	17.920423248133826	23.39490074360845	34.67290922700638	24.01176678125134
3	20.173974816799607	23.621606760019006	30.9005942192747	25.30382420390669
4	14.972688798513737	15.872122867827136	34.242223419454604	34.91296491420452
5	14.370046517245708	38.62393500617422	33.566634722811564	13.4393837537685
6	34.82428495825844	36.70190026206675	14.158676729775646	14.315138049899161
7	28.857776739470047	31.732803160693486	20.78432494370094	18.625095156135522
8	28.30074583339293	36.45636167436657	18.66681287734672	16.576079614893782
9	26.842771073806198	13.516541672427685	18.24462953868941	41.39605771507671
10	14.684320017036722	29.372772075031506	33.87256467836035	22.070343229571428
11	37.31677974155673	21.270952228838784	21.063873406979017	20.348394622625467
12	20.75254001325432	25.99411343088128	31.284635641396058	21.968710914468343
13	32.19185701866888	19.11029211940327	24.738052441956743	23.959798419971108
14	21.329436500860577	19.789059309090966	28.233679630150547	30.647824559897906
15	25.09682484437303	28.862703403689277	22.68744765419267	23.353024097745017
16	23.564314422888962	26.374261199022932	25.22881176805265	24.832612610035458
17	23.60905171249259	26.810668294055105	26.68559459274763	22.894685400704674
18	23.410952133483995	25.78401504062909	27.46702710777793	23.338005718108988
19	24.454371937720605	26.13515905973819	27.352204046539498	22.05826495600171
20	23.675323292473806	26.174969685122583	27.01711141730594	23.132595605097666
21	23.10851852028435	26.744396714073893	27.312870195111792	22.834214570529966
22	23.631142239152993	26.08517725661087	26.650074932973528	23.63360557126261
23	22.92805957767363	26.0440952340086	27.113975992842033	23.913869195475733
24	22.968903213297544	25.98068429776759	27.337662440860168	23.712750048074707
25	23.58449785372257	25.792676434175792	27.095461270856873	23.52736444124476
26	23.29032832243905	26.05275662755531	27.442552711334027	23.214362338671613
27	23.801827315651376	26.44236041250483	27.119856204974656	22.635956066869138
28	23.483739624206766	26.9185781329214	26.729378334437857	22.868303908433973
29	23.186788911509165	26.8672454702501	27.104281589055812	22.841684029184922
30	23.398397085957576	26.88552180525691	26.844280858002413	22.8718002507831
31	23.076097891228788	26.06435812716833	27.307466756935867	23.552077224667013
32	22.827539735136174	26.689488246727343	27.069477090216758	23.41349492791972
33	23.293347890831477	26.162017325965586	26.86978826468583	23.674846518517107
34	23.384173329582712	26.383002054895755	27.30277847969499	22.930046135826544
35	23.449014587693828	26.12673605316983	27.04992935799208	23.37432000114426
36	23.09707594532356	26.07214543512775	27.245088830934368	23.58568978861432
37	22.752686223934372	25.886998215276158	27.35236297119173	24.007952589597746
38	23.071886387944613	25.747382908289353	27.447956149509956	23.732774554256082
39	22.653676165593133	25.739992911960513	27.913446455900793	23.692884466545568
40	23.152143337322343	25.496917656369938	28.022150918028256	23.328788088279463
41	22.737826768950573	25.281892601898516	28.049406495886238	23.93087413326468
42	22.276468503517798	25.522981299336173	28.26014058474737	23.940409612398668
43	22.418785529592565	24.88092570431433	27.732351814681145	24.967936951411964
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	139.0
1	157.5
2	176.0
3	587.0
4	998.0
5	998.0
6	1606.0
7	2214.0
8	2614.5
9	3015.0
10	3855.5
11	4696.0
12	4696.0
13	7687.0
14	10678.0
15	18702.0
16	26726.0
17	21333.5
18	15941.0
19	15941.0
20	16356.5
21	16772.0
22	13843.5
23	10915.0
24	12250.0
25	13585.0
26	13585.0
27	15974.0
28	18363.0
29	20439.5
30	22516.0
31	24793.0
32	27070.0
33	27070.0
34	30470.0
35	33870.0
36	38850.0
37	43830.0
38	49318.0
39	54806.0
40	54806.0
41	61490.0
42	68174.0
43	75059.5
44	81945.0
45	88786.5
46	95628.0
47	95628.0
48	102238.0
49	108848.0
50	110192.0
51	111536.0
52	111617.5
53	111699.0
54	111699.0
55	103634.0
56	95569.0
57	86801.5
58	78034.0
59	70917.0
60	63800.0
61	63800.0
62	55748.0
63	47696.0
64	40372.5
65	33049.0
66	27394.5
67	21740.0
68	21740.0
69	17694.5
70	13649.0
71	11096.5
72	8544.0
73	6811.5
74	5079.0
75	5079.0
76	4075.5
77	3072.0
78	2475.0
79	1878.0
80	1472.0
81	1066.0
82	1066.0
83	830.0
84	594.0
85	459.5
86	325.0
87	230.0
88	135.0
89	135.0
90	98.0
91	61.0
92	38.0
93	15.0
94	10.0
95	5.0
96	5.0
97	5.5
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1258458.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.94553873998584
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.0093369940884	64.03824702928641
2	6.372469226530611	9.169414632134513
3	1.8427955142222543	3.977427481750478
4	0.786380069937917	2.263061511442847
5	0.46545066419820874	1.674354939631218
6	0.3126433703754865	1.3495977429089585
7	0.2198403789829879	1.1071574151913606
8	0.16047726023347444	0.9236498354411378
9	0.11587618517789708	0.7503097112782351
>10	0.6631151406713931	8.348524628385636
>50	0.03676948707770833	1.8255330743852511
>100	0.013073100184385912	1.743759811939884
>500	0.0011078801995731216	0.5343559815295339
>1k	5.539400997865608E-4	1.1477016375363602
>5k	0.0	0.0
>10k+	1.1078801995731214E-4	1.1469045671581837
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14389	1.143383410491252	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4602	0.36568562478843153	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3780	0.30036759272061525	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3102	0.24649213561358424	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1565	0.12435854037242403	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	1350	0.10727414025736258	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	7.946232611656487E-5	0.0	0.0	0.0
6	0.0	7.946232611656487E-5	0.0	0.0	0.0
7	0.0	7.946232611656487E-5	0.0	0.0	0.0
8	0.0	7.946232611656487E-5	0.0	0.0	0.0
9	0.0	7.946232611656487E-5	0.0	0.0	0.0
10	7.946232611656487E-5	7.946232611656487E-5	0.0	0.0	0.0
11	7.946232611656487E-5	7.946232611656487E-5	0.0	7.946232611656487E-5	0.0
12	7.946232611656487E-5	7.946232611656487E-5	0.0	7.946232611656487E-5	0.0
13	7.946232611656487E-5	7.946232611656487E-5	0.0	7.946232611656487E-5	0.0
14	7.946232611656487E-5	7.946232611656487E-5	0.0	3.178493044662595E-4	0.0
15	7.946232611656487E-5	7.946232611656487E-5	0.0	3.9731163058282436E-4	0.0
16	7.946232611656487E-5	7.946232611656487E-5	0.0	4.7677395669938925E-4	0.0
17	1.5892465223312974E-4	7.946232611656487E-5	0.0	7.151609350490839E-4	0.0
18	1.5892465223312974E-4	7.946232611656487E-5	0.0	8.740855872822137E-4	0.0
19	1.5892465223312974E-4	7.946232611656487E-5	0.0	0.0011124725656319083	7.946232611656487E-5
20	1.5892465223312974E-4	7.946232611656487E-5	0.0	0.0016687088484478624	7.946232611656487E-5
21	1.5892465223312974E-4	7.946232611656487E-5	0.0	0.002622256761846641	7.946232611656487E-5
22	2.3838697834969462E-4	7.946232611656487E-5	0.0	0.004926664219227023	7.946232611656487E-5
23	3.178493044662595E-4	7.946232611656487E-5	0.0	0.005880212132625801	7.946232611656487E-5
24	3.178493044662595E-4	7.946232611656487E-5	0.0	0.009138167503404961	7.946232611656487E-5
25	3.178493044662595E-4	7.946232611656487E-5	0.0	0.011124725656319083	7.946232611656487E-5
26	3.178493044662595E-4	7.946232611656487E-5	0.0	0.013746982418165724	7.946232611656487E-5
27	3.178493044662595E-4	7.946232611656487E-5	0.0	0.023123536899920378	7.946232611656487E-5
28	3.178493044662595E-4	7.946232611656487E-5	0.0	0.08327651777015999	7.946232611656487E-5
29	3.178493044662595E-4	7.946232611656487E-5	0.0	0.2115287121222957	7.946232611656487E-5
30	3.178493044662595E-4	7.946232611656487E-5	0.0	0.3575804675245419	7.946232611656487E-5
31	3.178493044662595E-4	7.946232611656487E-5	0.0	0.7354238282088079	7.946232611656487E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAA	30	3.5996715E-4	30.833332	10
GTATTAG	70	6.5938366E-9	26.42857	1
ATACTAG	70	5.10256E-6	21.142857	1
TATCTCG	45	0.003826006	20.555557	5
GTCGCGT	55	5.1434664E-4	20.181818	8
ACGGTAG	65	6.902797E-5	19.923077	19
GTATTAT	105	2.2599124E-8	19.38095	1
AACGGTA	70	1.2193143E-4	18.5	18
TAGTACG	50	0.0070352433	18.5	4
TATAGTG	155	2.0008883E-11	17.903225	5
ACCGTCG	95	3.606936E-6	17.526316	8
CTTATAC	1105	0.0	16.742083	37
ATAATAC	115	1.2423479E-6	16.086956	3
AACGCAG	6585	0.0	15.255126	5
GTAATAC	100	1.0934945E-4	14.8	3
GTAGGAC	125	2.959101E-6	14.799999	3
TAATACT	115	2.210063E-5	14.478261	4
TGAACCG	115	2.210063E-5	14.478261	5
CGGTAGT	90	8.274781E-4	14.388889	20
CTAGAGT	170	2.4365363E-8	14.1470585	4
>>END_MODULE