FastQCFastQC Report
Thu 9 Feb 2017
SRR2727320.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727320.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2773156
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT290641.0480477838246387No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT112220.40466529830994No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT100370.3619342006003268No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76600.27621958519463025No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA37620.1356577127287466No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30540.11012723409718025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC26800.016.29104637
TATACCG1151.2436667E-616.0869565
CAATACT5350.015.2149524
AACGCAG117750.014.8628455
TAGACGG908.2792115E-414.3888895
CCCGACT7100.014.07042212
ACCCGAC6700.013.80597111
TAATACT5250.013.7428574
TTAGACT3100.013.7258074
TTATACT6150.013.5365854
TCGTTAC3600.013.36111223
ACGCAGA132050.013.3233626
GTCCTAC3500.013.2142861
GGAGTGT6600.013.1742426
CGCAGAG133650.013.1361767
GTGTTAT3400.013.0588241
TATACAC11550.012.97402637
AGAGTAC137250.012.85901510
TAAGACT3600.012.8472224
CGAACCG1307.004765E-512.80769318
Produced by FastQC (version 0.11.5)