##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727320.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2773156
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32146009816974	33.0	31.0	34.0	30.0	34.0
2	32.4600895153392	34.0	31.0	34.0	31.0	34.0
3	32.554180507695925	34.0	31.0	34.0	31.0	34.0
4	36.089201977818774	37.0	35.0	37.0	35.0	37.0
5	35.89640178915286	37.0	35.0	37.0	35.0	37.0
6	35.91651641667472	37.0	35.0	37.0	35.0	37.0
7	35.88046471240709	37.0	35.0	37.0	35.0	37.0
8	35.83900905682911	37.0	35.0	37.0	35.0	37.0
9	37.67349150210086	39.0	37.0	39.0	35.0	39.0
10	37.49859870847511	39.0	37.0	39.0	35.0	39.0
11	37.614147923881674	39.0	37.0	39.0	35.0	39.0
12	37.53397428777898	39.0	37.0	39.0	35.0	39.0
13	37.617252329115274	39.0	37.0	39.0	35.0	39.0
14	38.82921191595424	40.0	38.0	41.0	36.0	41.0
15	38.75612082407192	40.0	38.0	41.0	35.0	41.0
16	38.72691006203762	40.0	38.0	41.0	35.0	41.0
17	38.66963560650753	40.0	38.0	41.0	35.0	41.0
18	38.65053065893156	40.0	38.0	41.0	35.0	41.0
19	38.710740758904294	40.0	38.0	41.0	34.0	41.0
20	38.675279717405004	40.0	38.0	41.0	34.0	41.0
21	38.6316939977412	40.0	38.0	41.0	34.0	41.0
22	38.58883092043866	40.0	38.0	41.0	34.0	41.0
23	38.51722802467658	40.0	38.0	41.0	34.0	41.0
24	38.47902462032428	40.0	38.0	41.0	34.0	41.0
25	38.361579370219346	40.0	38.0	41.0	34.0	41.0
26	38.246604590581995	40.0	38.0	41.0	34.0	41.0
27	38.15121471709489	40.0	38.0	41.0	34.0	41.0
28	38.05955669280776	40.0	38.0	41.0	33.0	41.0
29	37.95650406973138	40.0	37.0	41.0	33.0	41.0
30	37.878782513497256	40.0	37.0	41.0	33.0	41.0
31	37.767737191849285	40.0	37.0	41.0	33.0	41.0
32	37.66439500698843	40.0	37.0	41.0	33.0	41.0
33	37.552465133587866	40.0	37.0	41.0	33.0	41.0
34	37.44937608991344	40.0	36.0	41.0	32.0	41.0
35	37.360355493884946	40.0	36.0	41.0	32.0	41.0
36	37.26219116414655	39.0	36.0	41.0	32.0	41.0
37	37.16372933942411	39.0	36.0	41.0	31.0	41.0
38	37.05599180139884	39.0	36.0	41.0	31.0	41.0
39	36.92815550225087	39.0	36.0	41.0	31.0	41.0
40	36.789809877266194	39.0	35.0	41.0	31.0	41.0
41	36.66256928928629	39.0	35.0	41.0	30.0	41.0
42	36.52649941077963	39.0	35.0	41.0	30.0	41.0
43	34.86070491526622	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	14.0
12	7.0
13	7.0
14	10.0
15	12.0
16	15.0
17	26.0
18	53.0
19	130.0
20	289.0
21	579.0
22	1163.0
23	2161.0
24	3773.0
25	6163.0
26	9483.0
27	14347.0
28	21160.0
29	29332.0
30	39308.0
31	50976.0
32	66186.0
33	87281.0
34	117224.0
35	161244.0
36	238647.0
37	402166.0
38	751334.0
39	770060.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.38159988114624	17.686166952021452	12.48570221076636	25.44653095606594
2	16.446171798485192	21.381811913934882	36.76670190930478	25.40531437827515
3	18.420312452671254	22.90037055254014	31.22586684629354	27.453450148495072
4	12.961225405278318	15.023785174725116	35.856186958108374	36.15880246188819
5	14.015223088784042	37.793690654258185	34.79551817496023	13.39556808199755
6	34.68571548084565	36.66090187497566	14.785031927522288	13.868350716656403
7	29.232037433162795	31.223198406436563	21.461792989647897	18.082971170752746
8	27.938132582516097	36.30116733425743	19.086520916962478	16.674179166263997
9	26.554871056658914	13.585856691798082	18.710595437112083	41.14867681443092
10	14.634986275564735	28.166464490277505	34.784411695555534	22.414137538602226
11	36.47609438488134	21.475099128934687	21.972078022296618	20.076728463887356
12	20.721156689346003	25.634836265972776	31.729805319282434	21.914201725398787
13	31.657360783165462	19.189688571432693	25.124226693341452	24.028723952060396
14	21.416393452081312	19.659406106255833	28.126401832424865	30.797798609237994
15	24.852550667903284	28.51318137169348	22.95233300975495	23.681934950648287
16	23.666068551498725	26.37651830621862	25.355551580942436	24.601861561340222
17	23.231437394794956	26.99736329294133	26.894952898430525	22.876246413833194
18	23.10169352174923	26.014511985622157	27.66721381703734	23.216580675591274
19	24.10830836779467	26.213058334980072	27.535089984119175	22.14354331310608
20	23.33482140925357	26.177719536874232	27.48691382670142	23.000545227170775
21	23.029898065597465	26.564571196138985	27.431525669670226	22.97400506859333
22	23.577469136247654	25.901211471695063	27.042618590515644	23.47870080154164
23	23.0116156465774	25.973872367800443	27.259555538887824	23.754956446734337
24	23.00840630674942	26.01386290565695	27.38598189211137	23.591748895482258
25	23.451836102981584	25.796060517331153	27.281299717722334	23.470803661964922
26	22.960698929306535	26.156155658030055	27.750620592566737	23.13252482009667
27	23.535639538489722	26.53363171779734	27.253425339216403	22.677303404496538
28	23.33601138918979	26.558657356455967	27.19569328231084	22.909637972043402
29	22.858540954782207	26.780426344569147	27.494053706318724	22.866978994329926
30	23.04558416475669	26.7677332252495	27.255805299088838	22.930877310904975
31	22.988068467839533	26.099361161074242	27.429217829793924	23.483352541292305
32	22.455101696406548	26.733512287083744	27.45424346845255	23.35714254805716
33	22.950457889855457	26.3197238092628	27.25667073904245	23.47314756183929
34	23.199560356503564	26.189078436265394	27.780514330964433	22.83084687626661
35	22.92608133116204	26.362743386956954	27.515004565195756	23.19617071668525
36	22.785194918713554	26.017757385448203	27.734177233448097	23.462870462390143
37	22.515249773182614	25.681966683446582	27.885340745345736	23.917442798025064
38	22.48604117474819	25.716800641579486	28.22026600739374	23.57689217627858
39	22.224534068764974	25.569711909463443	28.581695368021126	23.624058653750456
40	22.449512396706137	25.482554894135056	28.843346713996613	23.22458599516219
41	22.132833493680124	25.20734498888631	29.055054962649052	23.60476655478451
42	21.60668206188184	25.45345447569484	28.99717866575122	23.942684796672093
43	21.354189955415418	24.87653056661796	29.08004454130961	24.689234936657005
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	367.0
1	481.0
2	595.0
3	2595.0
4	4595.0
5	4595.0
6	5994.5
7	7394.0
8	6768.5
9	6143.0
10	7996.5
11	9850.0
12	9850.0
13	15978.5
14	22107.0
15	35995.0
16	49883.0
17	38931.0
18	27979.0
19	27979.0
20	30860.0
21	33741.0
22	27820.5
23	21900.0
24	26190.0
25	30480.0
26	30480.0
27	37060.0
28	43640.0
29	49358.0
30	55076.0
31	62299.0
32	69522.0
33	69522.0
34	78616.5
35	87711.0
36	98826.0
37	109941.0
38	122922.0
39	135903.0
40	135903.0
41	148397.0
42	160891.0
43	172691.5
44	184492.0
45	196902.0
46	209312.0
47	209312.0
48	221223.0
49	233134.0
50	236096.0
51	239058.0
52	235526.0
53	231994.0
54	231994.0
55	215735.0
56	199476.0
57	182992.0
58	166508.0
59	152215.0
60	137922.0
61	137922.0
62	119962.5
63	102003.0
64	85924.5
65	69846.0
66	57991.0
67	46136.0
68	46136.0
69	37754.5
70	29373.0
71	24071.5
72	18770.0
73	15241.0
74	11712.0
75	11712.0
76	9387.0
77	7062.0
78	5549.5
79	4037.0
80	3227.0
81	2417.0
82	2417.0
83	1809.5
84	1202.0
85	888.5
86	575.0
87	415.5
88	256.0
89	256.0
90	178.5
91	101.0
92	65.0
93	29.0
94	23.5
95	18.0
96	18.0
97	11.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2773156.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.303497171610076
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.88416909535364	47.741334212505926
2	9.805657303651117	11.434082257384011
3	3.1084437186934153	5.436994186828519
4	1.4592180897211424	3.403100710672756
5	0.8914099327427532	2.598615824620612
6	0.5413721877399976	1.8938335090012384
7	0.39099143060591335	1.5957317437918954
8	0.28470571507462417	1.3279471082875662
9	0.21888008942553358	1.1485327207269023
>10	1.3040334248587073	13.911447012675051
>50	0.07833563825881988	3.10754367461668
>100	0.030683961680873555	3.0629772432766003
>500	0.001173200931636538	0.47265586584398916
>1k	6.792215919764939E-4	0.7777635254339489
>5k	6.174741745240854E-5	0.27576692301536976
>10k+	1.8524225235722562E-4	1.8116734813188582
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	29064	1.0480477838246387	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11222	0.40466529830994	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10037	0.3619342006003268	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7660	0.27621958519463025	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	3762	0.1356577127287466	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3054	0.11012723409718025	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.6059998067184104E-5	0.0
5	0.0	0.0	0.0	3.6059998067184104E-5	0.0
6	0.0	0.0	0.0	3.6059998067184104E-5	1.0817999420155231E-4
7	3.6059998067184104E-5	0.0	0.0	3.6059998067184104E-5	1.0817999420155231E-4
8	3.6059998067184104E-5	0.0	0.0	3.6059998067184104E-5	1.0817999420155231E-4
9	3.6059998067184104E-5	0.0	0.0	3.6059998067184104E-5	1.4423999226873641E-4
10	3.6059998067184104E-5	0.0	0.0	3.6059998067184104E-5	1.4423999226873641E-4
11	3.6059998067184104E-5	0.0	0.0	1.802999903359205E-4	1.4423999226873641E-4
12	3.6059998067184104E-5	0.0	0.0	2.1635998840310462E-4	1.4423999226873641E-4
13	7.211999613436821E-5	0.0	0.0	2.5241998647028874E-4	1.4423999226873641E-4
14	7.211999613436821E-5	0.0	0.0	2.8847998453747283E-4	2.5241998647028874E-4
15	7.211999613436821E-5	0.0	0.0	4.6877997487339333E-4	3.245399826046569E-4
16	1.0817999420155231E-4	0.0	0.0	6.130199671421297E-4	4.3271997680620924E-4
17	1.0817999420155231E-4	0.0	0.0	8.293799555452344E-4	4.3271997680620924E-4
18	1.4423999226873641E-4	0.0	0.0	9.375599497467867E-4	4.3271997680620924E-4
19	1.4423999226873641E-4	0.0	0.0	0.0016587599110904688	4.3271997680620924E-4
20	1.802999903359205E-4	0.0	0.0	0.0026323798589044396	4.3271997680620924E-4
21	2.1635998840310462E-4	0.0	0.0	0.003822359795121515	4.3271997680620924E-4
22	2.1635998840310462E-4	0.0	0.0	0.006743219638563428	4.6877997487339333E-4
23	2.1635998840310462E-4	0.0	0.0	0.008293799555452343	4.6877997487339333E-4
24	2.1635998840310462E-4	0.0	0.0	0.011539199381498912	4.6877997487339333E-4
25	2.1635998840310462E-4	0.0	0.0	0.01363067926939559	5.048399729405775E-4
26	2.1635998840310462E-4	0.0	0.0	0.016515479114770318	5.408999710077616E-4
27	2.1635998840310462E-4	0.0	0.0	0.02617955859677566	5.408999710077616E-4
28	2.1635998840310462E-4	0.0	0.0	0.09386417496888022	5.408999710077616E-4
29	2.1635998840310462E-4	0.0	0.0	0.21895630826394188	5.408999710077616E-4
30	2.5241998647028874E-4	0.0	0.0	0.3636650805075517	5.408999710077616E-4
31	2.5241998647028874E-4	0.0	0.0	0.7973586772615749	5.408999710077616E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2680	0.0	16.291046	37
TATACCG	115	1.2436667E-6	16.086956	5
CAATACT	535	0.0	15.214952	4
AACGCAG	11775	0.0	14.862845	5
TAGACGG	90	8.2792115E-4	14.388889	5
CCCGACT	710	0.0	14.070422	12
ACCCGAC	670	0.0	13.805971	11
TAATACT	525	0.0	13.742857	4
TTAGACT	310	0.0	13.725807	4
TTATACT	615	0.0	13.536585	4
TCGTTAC	360	0.0	13.361112	23
ACGCAGA	13205	0.0	13.323362	6
GTCCTAC	350	0.0	13.214286	1
GGAGTGT	660	0.0	13.174242	6
CGCAGAG	13365	0.0	13.136176	7
GTGTTAT	340	0.0	13.058824	1
TATACAC	1155	0.0	12.974026	37
AGAGTAC	13725	0.0	12.859015	10
TAAGACT	360	0.0	12.847222	4
CGAACCG	130	7.004765E-5	12.807693	18
>>END_MODULE