FastQCFastQC Report
Thu 9 Feb 2017
SRR2727319.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727319.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2061648
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT306021.4843465033798204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT112790.5470866025626101No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT99040.4803923851210294No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80050.38828160772352993No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA36400.17655778289989368No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30120.1460967148611208No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24210.11743032758259411No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22830.11073665339573002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC1653.6379788E-1217.9393963
ACCGTCC1351.1532393E-917.8148148
CTTATAC21500.016.7790737
CTATACT2450.016.6122444
CAATACT2600.016.3653854
AACGCAG119750.015.8350735
GCTAGTA1201.9372164E-615.4166662
AGTCGGT2400.015.41666611
GTGTACG1101.4529009E-515.1363641
ATTACGG2450.015.1020425
TATACTG2550.014.5098045
CCGCTTA1152.2114156E-514.47826125
TCTATAC2301.8189894E-1214.4782613
GACCGTG3850.014.4155847
TACGCCG908.2779414E-414.38888910
CGACCGA1551.2114833E-714.32258118
CGTTACG1551.2114833E-714.32258124
TACACCG1551.2114833E-714.3225815
ACGCAGA133450.014.251036
TTACGGA4550.014.23076926
Produced by FastQC (version 0.11.5)