FastQCFastQC Report
Thu 9 Feb 2017
SRR2727318.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727318.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3710104
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT310430.8367150893883297No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT133110.35877700463383233No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT121500.32748408130877193No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77330.20843081487742662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC33250.017.08120337
AACGCAG127250.016.1084485
CCGCTAG700.00259395415.85714236
ACGGATG10800.015.75925928
TACGGAT11300.015.3893827
GAGTGTC13050.015.0268197
GGAGTGT15050.014.8737546
TTAGACT5950.014.6134454
GGTATCA74800.014.5922451
CGGATGG12350.014.53036529
ACGCAGA143200.014.4434376
CGCAGAG144150.014.2840797
TGTTACG4800.014.26041624
TTACGGA13150.014.06844126
GGACCGT3600.013.8756
TATACTG6150.013.8373995
TCTATAC6050.013.7603313
AGAGTAC151000.013.59933810
GTTACGG10150.013.48768525
CAGAGTA154350.013.412059
Produced by FastQC (version 0.11.5)