##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727318.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3710104
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34887243053025	33.0	31.0	34.0	31.0	34.0
2	32.49470877366241	34.0	31.0	34.0	31.0	34.0
3	32.5854711889478	34.0	31.0	34.0	31.0	34.0
4	36.111115214020955	37.0	35.0	37.0	35.0	37.0
5	35.92319514493394	37.0	35.0	37.0	35.0	37.0
6	35.941214046829955	37.0	35.0	37.0	35.0	37.0
7	35.91208817866022	37.0	35.0	37.0	35.0	37.0
8	35.8654156325537	37.0	35.0	37.0	35.0	37.0
9	37.70125635292165	39.0	37.0	39.0	35.0	39.0
10	37.52407964844112	39.0	37.0	39.0	35.0	39.0
11	37.63355393811063	39.0	37.0	39.0	35.0	39.0
12	37.558801047086554	39.0	37.0	39.0	35.0	39.0
13	37.63452426131451	39.0	37.0	39.0	35.0	39.0
14	38.86625765746729	40.0	38.0	41.0	36.0	41.0
15	38.7928640275313	40.0	38.0	41.0	35.0	41.0
16	38.75940593579048	40.0	38.0	41.0	35.0	41.0
17	38.71484788566574	40.0	38.0	41.0	35.0	41.0
18	38.68317572768849	40.0	38.0	41.0	35.0	41.0
19	38.744845427513624	40.0	38.0	41.0	35.0	41.0
20	38.702543378837895	40.0	38.0	41.0	34.0	41.0
21	38.660215185342516	40.0	38.0	41.0	34.0	41.0
22	38.620207411975514	40.0	38.0	41.0	34.0	41.0
23	38.55227966655382	40.0	38.0	41.0	34.0	41.0
24	38.51243927393949	40.0	38.0	41.0	34.0	41.0
25	38.404882181200314	40.0	38.0	41.0	34.0	41.0
26	38.303386913143136	40.0	38.0	41.0	34.0	41.0
27	38.21507672022132	40.0	38.0	41.0	34.0	41.0
28	38.13051493974293	40.0	38.0	41.0	34.0	41.0
29	38.046352069915024	40.0	38.0	41.0	33.0	41.0
30	37.98459881448067	40.0	37.0	41.0	33.0	41.0
31	37.87871768554197	40.0	37.0	41.0	33.0	41.0
32	37.787472264928425	40.0	37.0	41.0	33.0	41.0
33	37.696679661810016	40.0	37.0	41.0	33.0	41.0
34	37.60259928023581	40.0	37.0	41.0	33.0	41.0
35	37.5429090397466	40.0	37.0	41.0	33.0	41.0
36	37.44785159661293	40.0	37.0	41.0	32.0	41.0
37	37.37702528015387	40.0	37.0	41.0	32.0	41.0
38	37.28378449768524	40.0	36.0	41.0	32.0	41.0
39	37.16788100818737	39.0	36.0	41.0	31.0	41.0
40	37.066719693032866	39.0	36.0	41.0	31.0	41.0
41	36.95555434564638	39.0	36.0	41.0	31.0	41.0
42	36.85351488799236	39.0	36.0	41.0	31.0	41.0
43	35.21326059862473	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	13.0
12	10.0
13	10.0
14	9.0
15	9.0
16	19.0
17	35.0
18	84.0
19	163.0
20	377.0
21	725.0
22	1433.0
23	2744.0
24	4690.0
25	7507.0
26	11720.0
27	17368.0
28	25382.0
29	36224.0
30	49358.0
31	64594.0
32	84757.0
33	110307.0
34	150813.0
35	208628.0
36	310182.0
37	522471.0
38	1018051.0
39	1082414.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.42174774615483	18.633143437488545	12.514420080946516	25.43068873541011
2	16.503607446044636	21.35613988179307	37.66678777737767	24.473464894784623
3	17.91049523140052	23.28902370391773	31.73425866229087	27.066222402390878
4	12.701827226406593	15.461965486681775	37.14068392691957	34.695523359992066
5	13.19704245487458	38.49115819933889	35.560674309938484	12.751125035848052
6	33.36984623611629	38.00351688254561	15.164561424693215	13.46207545664488
7	28.479579009105944	32.0421745589881	21.76761082708194	17.710635604824017
8	26.948651574187675	37.141923784346744	19.468025694158438	16.441398947307135
9	26.84593208168828	13.324100887737918	19.244474009353915	40.58549302121989
10	14.275529742562473	28.385107263839505	35.167585598678635	22.17177739491939
11	36.414046614326715	21.785346178975036	22.427538419408187	19.37306878729006
12	20.151429717334068	25.840731149315495	32.300900459933196	21.70693867341724
13	31.099613380110096	19.83712585954464	25.45869334121092	23.60456741913434
14	20.83421381179611	19.969197628961343	28.33831612267473	30.85827243656782
15	24.60548275735667	29.101340555413003	23.07134786518114	23.221828822049194
16	23.005231120205792	27.560575121344304	25.464515280434185	23.96967847801571
17	22.83628706904173	27.25397994234124	26.9124800814209	22.997252907196135
18	22.785506821372124	26.77676959998965	27.710894357678384	22.726829220959843
19	24.128191554738088	26.339180788463075	27.692323449693056	21.840304207105785
20	23.306300847631224	26.319370023050563	27.612083111416823	22.762246017901386
21	22.98558207532727	26.527450443437704	28.041127688064808	22.44583979317022
22	23.495594732654396	25.831297451500014	27.326861996321394	23.346245819524196
23	22.754699059649003	26.140480159046753	27.883396260589997	23.221424520714244
24	22.68451234790184	25.979649088004003	27.994040059254406	23.341798504839755
25	23.13749156357881	25.90792603118403	27.678954552217405	23.275627853019753
26	22.66082028967382	26.078622054799546	28.12093677158376	23.139620883942875
27	23.206384511054136	26.6413286527817	27.57844524034906	22.573841595815104
28	22.925610710643152	26.80334567440697	27.186488572827066	23.08455504212281
29	22.62602342144587	26.89436738161518	27.751540118551933	22.72806907838702
30	23.41198521658692	26.649980701349612	27.249829115302425	22.688204966761038
31	22.96765804947786	26.330528739895158	27.63011495095555	23.07169825967143
32	22.44945155176243	26.79428932450411	27.59849858656253	23.157760537170926
33	22.776207890668292	26.15630181795443	27.73167544629477	23.33581484508251
34	22.971997550472977	26.016952624508637	28.38750611842687	22.623543706591516
35	22.87545039168713	26.143471988925377	27.81593184449816	23.16514577488933
36	22.66502502355729	26.04220798123179	28.118807451219695	23.173959543991216
37	22.442443661956645	25.852779329096975	28.177053796874695	23.527723212071685
38	22.671736425717445	25.69008847191346	28.291740608888595	23.346434493480505
39	22.065796538318065	25.6386343886856	28.944175149807123	23.351393923189214
40	22.594326196785858	25.33427634373592	28.998917550559227	23.072479908918993
41	22.047980326157973	25.423007010046078	29.10646170565569	23.422550958140256
42	21.764322509557683	25.479501383249637	29.159425180534022	23.59675092665866
43	21.38034405504536	25.052640033810373	28.968891438083677	24.598124473060594
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	998.0
1	1112.5
2	1227.0
3	3649.5
4	6072.0
5	6072.0
6	7369.0
7	8666.0
8	7587.0
9	6508.0
10	8654.0
11	10800.0
12	10800.0
13	16659.0
14	22518.0
15	36993.5
16	51469.0
17	42042.0
18	32615.0
19	32615.0
20	36464.0
21	40313.0
22	36531.5
23	32750.0
24	39764.0
25	46778.0
26	46778.0
27	55893.5
28	65009.0
29	74663.5
30	84318.0
31	95890.0
32	107462.0
33	107462.0
34	121600.5
35	135739.0
36	151104.0
37	166469.0
38	184406.5
39	202344.0
40	202344.0
41	218478.0
42	234612.0
43	249208.5
44	263805.0
45	279260.5
46	294716.0
47	294716.0
48	305717.5
49	316719.0
50	317325.5
51	317932.0
52	306632.5
53	295333.0
54	295333.0
55	274571.0
56	253809.0
57	231404.0
58	208999.0
59	188731.5
60	168464.0
61	168464.0
62	145344.0
63	122224.0
64	101581.5
65	80939.0
66	66026.0
67	51113.0
68	51113.0
69	41317.0
70	31521.0
71	25482.5
72	19444.0
73	15708.0
74	11972.0
75	11972.0
76	9596.0
77	7220.0
78	5664.5
79	4109.0
80	3338.0
81	2567.0
82	2567.0
83	1959.5
84	1352.0
85	1002.5
86	653.0
87	508.0
88	363.0
89	363.0
90	240.0
91	117.0
92	75.0
93	33.0
94	31.0
95	29.0
96	29.0
97	15.0
98	1.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3710104.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.57739649614265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.16707074012912	54.998676956003465
2	8.253717935712848	10.660072314037052
3	2.482458664548322	4.809321523974653
4	1.1708263310552507	3.0243566483471556
5	0.6795320653370187	2.1941205807555684
6	0.44474348593861607	1.7232225858320787
7	0.3091633376773384	1.3975474407482222
8	0.22993644942229507	1.1878957810607038
9	0.18396310488952913	1.069187252860134
>10	0.9979176901775019	11.513539695403631
>50	0.05692137594871267	2.4749280417666895
>100	0.02245610577762569	2.464918799730136
>500	8.757090732461848E-4	0.3863511270322916
>1k	2.502025874793066E-4	0.36601968602032353
>5k	4.1700431246550775E-5	0.20824236550876632
>10k+	1.2510129373965233E-4	1.5215992009190913
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	31043	0.8367150893883297	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13311	0.35877700463383233	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12150	0.32748408130877193	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7733	0.20843081487742662	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.6953422329940077E-5
2	2.6953422329940077E-5	0.0	0.0	0.0	2.6953422329940077E-5
3	2.6953422329940077E-5	0.0	0.0	0.0	2.6953422329940077E-5
4	2.6953422329940077E-5	0.0	0.0	0.0	2.6953422329940077E-5
5	2.6953422329940077E-5	0.0	0.0	0.0	5.3906844659880154E-5
6	2.6953422329940077E-5	0.0	0.0	0.0	5.3906844659880154E-5
7	2.6953422329940077E-5	0.0	0.0	0.0	5.3906844659880154E-5
8	2.6953422329940077E-5	0.0	0.0	0.0	5.3906844659880154E-5
9	2.6953422329940077E-5	0.0	0.0	8.086026698982023E-5	5.3906844659880154E-5
10	2.6953422329940077E-5	0.0	0.0	1.3476711164970038E-4	8.086026698982023E-5
11	2.6953422329940077E-5	0.0	0.0	1.8867395630958053E-4	8.086026698982023E-5
12	2.6953422329940077E-5	0.0	0.0	2.425808009694607E-4	8.086026698982023E-5
13	2.6953422329940077E-5	0.0	0.0	3.7734791261916106E-4	8.086026698982023E-5
14	2.6953422329940077E-5	0.0	0.0	4.851616019389214E-4	1.0781368931976031E-4
15	2.6953422329940077E-5	0.0	0.0	7.00788980578442E-4	1.3476711164970038E-4
16	2.6953422329940077E-5	0.0	0.0	0.001051183470867663	1.6172053397964047E-4
17	2.6953422329940077E-5	0.0	0.0	0.0012937642718371238	1.6172053397964047E-4
18	2.6953422329940077E-5	0.0	0.0	0.0014285313834868241	1.6172053397964047E-4
19	2.6953422329940077E-5	2.6953422329940077E-5	0.0	0.0018597861407658653	1.6172053397964047E-4
20	2.6953422329940077E-5	2.6953422329940077E-5	0.0	0.002776202499983828	1.8867395630958053E-4
21	2.6953422329940077E-5	2.6953422329940077E-5	0.0	0.004447314684440113	2.425808009694607E-4
22	2.6953422329940077E-5	2.6953422329940077E-5	0.0	0.006549681626175439	2.425808009694607E-4
23	2.6953422329940077E-5	2.6953422329940077E-5	0.0	0.008301654077621543	3.50394490289221E-4
24	2.6953422329940077E-5	2.6953422329940077E-5	0.0	0.011212623689255073	4.3125475727904123E-4
25	2.6953422329940077E-5	2.6953422329940077E-5	0.0	0.012479434538762256	4.3125475727904123E-4
26	2.6953422329940077E-5	2.6953422329940077E-5	0.0	0.014312267257198181	4.3125475727904123E-4
27	2.6953422329940077E-5	2.6953422329940077E-5	0.0	0.021697504975601762	4.3125475727904123E-4
28	2.6953422329940077E-5	2.6953422329940077E-5	0.0	0.07520004830053281	4.3125475727904123E-4
29	2.6953422329940077E-5	2.6953422329940077E-5	0.0	0.1737148069164638	4.3125475727904123E-4
30	2.6953422329940077E-5	2.6953422329940077E-5	0.0	0.2916360296099516	4.3125475727904123E-4
31	5.3906844659880154E-5	2.6953422329940077E-5	0.0	0.6864228064765839	4.3125475727904123E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3325	0.0	17.081203	37
AACGCAG	12725	0.0	16.108448	5
CCGCTAG	70	0.002593954	15.857142	36
ACGGATG	1080	0.0	15.759259	28
TACGGAT	1130	0.0	15.38938	27
GAGTGTC	1305	0.0	15.026819	7
GGAGTGT	1505	0.0	14.873754	6
TTAGACT	595	0.0	14.613445	4
GGTATCA	7480	0.0	14.592245	1
CGGATGG	1235	0.0	14.530365	29
ACGCAGA	14320	0.0	14.443437	6
CGCAGAG	14415	0.0	14.284079	7
TGTTACG	480	0.0	14.260416	24
TTACGGA	1315	0.0	14.068441	26
GGACCGT	360	0.0	13.875	6
TATACTG	615	0.0	13.837399	5
TCTATAC	605	0.0	13.760331	3
AGAGTAC	15100	0.0	13.599338	10
GTTACGG	1015	0.0	13.487685	25
CAGAGTA	15435	0.0	13.41205	9
>>END_MODULE