FastQCFastQC Report
Thu 9 Feb 2017
SRR2727317.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727317.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3394513
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT571241.6828334432656467No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT207390.6109565643142331No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT203640.5999093242535822No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146650.43202073463851814No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA73610.21684995756386852No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60700.1788179924484013No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46090.1357779451721057No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT45670.1345406542853128No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43450.12800068816940752No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA40600.11960478572331289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGT7750.020.05161511
TCGCGTG500.00703764318.511
CGGTGAT8250.018.3878814
CGCCTTA7200.016.95833425
TATACTG5250.016.9142865
CTTATAC36950.016.67253137
GTCGGTG9900.016.63131312
TCGGTGA9550.016.4659713
TACGGAT10750.016.00465227
ACGGATG11800.015.99152628
CTCGCCT8550.015.79532123
TAGTACT2950.015.6779664
GTTACGG8100.015.53086425
TCGGTAA2055.456968E-1215.34146422
CGGTAAT2006.184564E-1114.823
TTACGGA12100.014.67768626
TCGCCTT9500.014.60526324
GTGATTC10700.014.52336416
ATGGTTG20550.014.22384532
TATACAC13400.014.22014937
Produced by FastQC (version 0.11.5)