##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727317.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3394513
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23852611552821	33.0	31.0	34.0	30.0	34.0
2	32.36773758120826	34.0	31.0	34.0	30.0	34.0
3	32.46126587230628	34.0	31.0	34.0	30.0	34.0
4	36.00948530761261	37.0	35.0	37.0	35.0	37.0
5	35.81736054626982	37.0	35.0	37.0	35.0	37.0
6	35.84793400408247	37.0	35.0	37.0	35.0	37.0
7	35.80944070622207	37.0	35.0	37.0	35.0	37.0
8	35.76751422074389	37.0	35.0	37.0	35.0	37.0
9	37.59777116776397	39.0	37.0	39.0	35.0	39.0
10	37.43320853388984	39.0	37.0	39.0	35.0	39.0
11	37.54872466241844	39.0	37.0	39.0	35.0	39.0
12	37.47091291151337	39.0	37.0	39.0	35.0	39.0
13	37.55524223946115	39.0	37.0	39.0	35.0	39.0
14	38.70006036211969	40.0	38.0	41.0	35.0	41.0
15	38.629974903616514	40.0	38.0	41.0	34.0	41.0
16	38.595407942170205	40.0	38.0	41.0	34.0	41.0
17	38.55978221323648	40.0	38.0	41.0	34.0	41.0
18	38.559486147202854	40.0	38.0	41.0	34.0	41.0
19	38.625288517086254	40.0	38.0	41.0	34.0	41.0
20	38.59506650880406	40.0	38.0	41.0	34.0	41.0
21	38.54742138268435	40.0	38.0	41.0	34.0	41.0
22	38.50658931045484	40.0	38.0	41.0	34.0	41.0
23	38.42387523630047	40.0	38.0	41.0	34.0	41.0
24	38.387034016367004	40.0	38.0	41.0	34.0	41.0
25	38.26403286715944	40.0	38.0	41.0	34.0	41.0
26	38.1293298920935	40.0	37.0	41.0	34.0	41.0
27	38.021978115859326	40.0	37.0	41.0	33.0	41.0
28	37.90357144014473	40.0	37.0	41.0	33.0	41.0
29	37.772790677189924	40.0	37.0	41.0	33.0	41.0
30	37.664069632374364	40.0	37.0	41.0	33.0	41.0
31	37.514822892120314	39.0	36.0	41.0	33.0	41.0
32	37.38495742982867	39.0	36.0	41.0	33.0	41.0
33	37.24152212703265	39.0	36.0	41.0	32.0	41.0
34	37.10220405695898	39.0	35.0	41.0	31.0	41.0
35	36.98221571106076	39.0	35.0	41.0	31.0	41.0
36	36.8418553707115	39.0	35.0	41.0	31.0	41.0
37	36.70975689296226	39.0	35.0	41.0	31.0	41.0
38	36.572913404662174	39.0	35.0	41.0	30.0	41.0
39	36.417340572859786	39.0	35.0	40.0	30.0	41.0
40	36.24049281885207	39.0	35.0	40.0	30.0	41.0
41	36.06880987051751	39.0	35.0	40.0	29.0	41.0
42	35.88207027046295	39.0	35.0	40.0	28.0	41.0
43	34.238522875004456	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	13.0
11	17.0
12	15.0
13	9.0
14	7.0
15	9.0
16	10.0
17	28.0
18	93.0
19	195.0
20	445.0
21	927.0
22	1840.0
23	3179.0
24	5534.0
25	9065.0
26	14278.0
27	21466.0
28	31406.0
29	43038.0
30	56261.0
31	70261.0
32	89349.0
33	116097.0
34	153627.0
35	209381.0
36	308951.0
37	522130.0
38	890878.0
39	845998.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.54317511819811	16.71948229392552	12.998506707736867	24.738835880139508
2	16.288345338491855	22.34712313666202	36.18130200120017	25.183229523645956
3	19.076197380890868	23.42562835964982	30.93401026892517	26.564163990534134
4	13.264023440181258	14.650702471900976	34.35208526230419	37.733188825613574
5	14.577643390966537	38.00960550158447	33.74439868104792	13.668352426401079
6	35.42923535717789	35.245350363955005	14.517929376025368	14.807484902841733
7	29.18312582688592	30.91506793463451	21.38100516922457	18.520801069255
8	29.037096042937527	35.51204546867253	19.07817115444837	16.372687333941567
9	26.933230186480355	13.595381723387126	18.58296020666293	40.88842788346959
10	14.94709255790153	28.53652350130932	33.713083437889324	22.803300502899827
11	36.27162423593605	21.604247796370203	21.250912870270344	20.8732150974234
12	20.976764560925236	25.852014707264342	31.37622392372632	21.794996808084104
13	32.494175158557354	18.58107481102591	25.082537612906474	23.842212417510257
14	21.56704069184593	19.54353982441664	28.82009878883952	30.06932069489791
15	25.50003490927859	28.164570293293913	23.292501752092274	23.042893045335223
16	23.205272744573374	25.84956958479758	25.892256120391938	25.052901550237106
17	23.104757589674865	26.64455843886884	27.563512056074025	22.687171915382265
18	22.88027767164244	25.378927698907027	28.531957308750915	23.20883732069961
19	23.921752545946944	26.178335449002553	28.351283379972326	21.548628625078177
20	22.918103421610112	25.95915820619924	28.25886953445163	22.86386883773902
21	22.261985739927937	26.801399788423257	28.527184901044716	22.40942957060409
22	23.03370174160476	25.95049716999169	27.555911554912292	23.459889533491253
23	22.283255359458042	25.867598680576563	28.21774434211918	23.631401617846212
24	22.368952483021864	25.777924550590907	28.492305081759888	23.360817884627338
25	22.86920097227496	25.73417747995073	27.987902830243982	23.40871871753032
26	22.50679257967196	26.094582639689406	28.799477274059637	22.599147506579
27	23.287994478147528	26.549522715040418	28.199950920794826	21.962531886017228
28	22.755753181678788	27.019398658953435	27.87256964401079	22.35227851535699
29	22.417471961368243	26.949403345929152	28.551341532644003	22.081783160058603
30	22.56694848421556	26.845117399756607	28.342740180992088	22.245193935035747
31	22.290031000028577	26.30598262549002	28.44210642292429	22.961879951557115
32	21.79676436649381	26.779216930381473	28.339853168922907	23.084165534201816
33	22.412493338514246	26.259584217235286	28.168782974170377	23.1591394700801
34	22.73224465482972	26.52630878125964	28.473510044003365	22.267936519907273
35	22.424895706689004	26.585286313530098	28.315343025641678	22.674474954139225
36	22.180000489024494	26.320034714847168	28.654773158918523	22.845191637209812
37	21.628728480344602	26.099620181157064	28.803601577015613	23.468049761482725
38	21.99311064650511	25.900652022838035	28.974436097313514	23.131801233343342
39	21.521408225568734	25.90739820410174	29.4249867359471	23.14620683438243
40	22.116898653798057	25.753738459684794	29.462841945221598	22.666520941295556
41	21.647494058794294	25.588589585604772	29.574433799487586	23.18948255611335
42	20.971402967082465	25.91175818151234	29.630759994143492	23.486078857261706
43	20.715489968664137	25.404675133075056	29.518195982752164	24.361638915508646
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	211.0
1	467.5
2	724.0
3	4651.0
4	8578.0
5	8578.0
6	11704.0
7	14830.0
8	13521.0
9	12212.0
10	15415.5
11	18619.0
12	18619.0
13	30441.0
14	42263.0
15	69167.0
16	96071.0
17	74767.5
18	53464.0
19	53464.0
20	54933.0
21	56402.0
22	41530.5
23	26659.0
24	29837.0
25	33015.0
26	33015.0
27	38965.0
28	44915.0
29	51237.5
30	57560.0
31	63785.0
32	70010.0
33	70010.0
34	77858.5
35	85707.0
36	98591.5
37	111476.0
38	125965.5
39	140455.0
40	140455.0
41	156662.0
42	172869.0
43	189420.0
44	205971.0
45	226784.0
46	247597.0
47	247597.0
48	268782.5
49	289968.0
50	298692.5
51	307417.0
52	306894.0
53	306371.0
54	306371.0
55	282016.5
56	257662.0
57	230418.5
58	203175.0
59	187080.0
60	170985.0
61	170985.0
62	148739.5
63	126494.0
64	106436.5
65	86379.0
66	71380.0
67	56381.0
68	56381.0
69	45870.0
70	35359.0
71	28752.5
72	22146.0
73	18046.5
74	13947.0
75	13947.0
76	11158.5
77	8370.0
78	6660.5
79	4951.0
80	3886.0
81	2821.0
82	2821.0
83	2089.0
84	1357.0
85	1014.5
86	672.0
87	485.5
88	299.0
89	299.0
90	203.0
91	107.0
92	67.0
93	27.0
94	18.5
95	10.0
96	10.0
97	7.0
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3394513.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.084291408443676
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.69998072338572	42.83901293375995
2	10.125113116935031	10.749689104856694
3	3.3046786613228027	5.262795752067755
4	1.635896316307546	3.4736158707547733
5	0.9604497138735534	2.5492396247210025
6	0.6137115897620297	1.9547066923000092
7	0.4637718478320647	1.7233299942144806
8	0.3145035145078866	1.3356156970493078
9	0.2455565464910633	1.1731675744064383
>10	1.4979092446353839	14.527679874591035
>50	0.0904414564316691	3.2519865247836948
>100	0.04223550026274104	4.135869929475579
>500	0.003484244231077585	1.31796239525524
>1k	0.001935691237912563	1.9966725128530767
>5k	1.1061092788071788E-4	0.39431419673768076
>10k+	2.2122185576143577E-4	3.3143413221733495
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	57124	1.6828334432656467	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	20739	0.6109565643142331	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	20364	0.5999093242535822	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14665	0.43202073463851814	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	7361	0.21684995756386852	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6070	0.1788179924484013	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4609	0.1357779451721057	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	4567	0.1345406542853128	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4345	0.12800068816940752	No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	4060	0.11960478572331289	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.891861365680438E-5
2	0.0	0.0	0.0	2.945930682840219E-5	5.891861365680438E-5
3	0.0	0.0	0.0	2.945930682840219E-5	5.891861365680438E-5
4	0.0	0.0	0.0	8.837792048520657E-5	5.891861365680438E-5
5	0.0	0.0	0.0	1.1783722731360876E-4	5.891861365680438E-5
6	0.0	0.0	0.0	1.1783722731360876E-4	5.891861365680438E-5
7	2.945930682840219E-5	0.0	0.0	1.4729653414201094E-4	5.891861365680438E-5
8	5.891861365680438E-5	0.0	0.0	1.4729653414201094E-4	5.891861365680438E-5
9	8.837792048520657E-5	0.0	0.0	2.3567445462721753E-4	1.1783722731360876E-4
10	1.1783722731360876E-4	0.0	0.0	2.3567445462721753E-4	1.1783722731360876E-4
11	1.1783722731360876E-4	0.0	0.0	3.535116819408263E-4	1.1783722731360876E-4
12	1.4729653414201094E-4	0.0	0.0	4.4188960242603283E-4	1.1783722731360876E-4
13	1.4729653414201094E-4	0.0	0.0	5.008082160828372E-4	1.1783722731360876E-4
14	2.0621514779881533E-4	0.0	0.0	7.364826707100548E-4	1.4729653414201094E-4
15	2.0621514779881533E-4	0.0	0.0	9.721571253372722E-4	1.7675584097041314E-4
16	2.3567445462721753E-4	0.0	0.0	0.0016202618755621204	2.0621514779881533E-4
17	2.3567445462721753E-4	0.0	0.0	0.002179988705301762	2.0621514779881533E-4
18	2.3567445462721753E-4	0.0	0.0	0.002621878307727795	2.0621514779881533E-4
19	2.3567445462721753E-4	0.0	0.0	0.003711872660378676	2.0621514779881533E-4
20	2.3567445462721753E-4	0.0	0.0	0.005715105524710024	2.0621514779881533E-4
21	2.945930682840219E-4	0.0	0.0	0.00927968165094669	2.0621514779881533E-4
22	2.945930682840219E-4	0.0	0.0	0.014552897573230682	2.3567445462721753E-4
23	2.945930682840219E-4	0.0	0.0	0.01814693300629575	3.535116819408263E-4
24	3.2405237511242405E-4	0.0	0.0	0.025629596940709903	3.535116819408263E-4
25	3.2405237511242405E-4	0.0	0.0	0.03152145830639034	3.535116819408263E-4
26	3.2405237511242405E-4	0.0	0.0	0.039387093229573726	3.535116819408263E-4
27	3.2405237511242405E-4	0.0	0.0	0.0524964847682127	3.535116819408263E-4
28	3.8297098876922844E-4	2.945930682840219E-5	0.0	0.13168310152295779	3.535116819408263E-4
29	3.8297098876922844E-4	2.945930682840219E-5	0.0	0.2974800803532053	3.535116819408263E-4
30	3.8297098876922844E-4	2.945930682840219E-5	0.0	0.5021338848901153	3.535116819408263E-4
31	3.8297098876922844E-4	2.945930682840219E-5	0.0	0.9889489302294615	3.535116819408263E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTCGGT	775	0.0	20.051615	11
TCGCGTG	50	0.007037643	18.5	11
CGGTGAT	825	0.0	18.38788	14
CGCCTTA	720	0.0	16.958334	25
TATACTG	525	0.0	16.914286	5
CTTATAC	3695	0.0	16.672531	37
GTCGGTG	990	0.0	16.631313	12
TCGGTGA	955	0.0	16.46597	13
TACGGAT	1075	0.0	16.004652	27
ACGGATG	1180	0.0	15.991526	28
CTCGCCT	855	0.0	15.795321	23
TAGTACT	295	0.0	15.677966	4
GTTACGG	810	0.0	15.530864	25
TCGGTAA	205	5.456968E-12	15.341464	22
CGGTAAT	200	6.184564E-11	14.8	23
TTACGGA	1210	0.0	14.677686	26
TCGCCTT	950	0.0	14.605263	24
GTGATTC	1070	0.0	14.523364	16
ATGGTTG	2055	0.0	14.223845	32
TATACAC	1340	0.0	14.220149	37
>>END_MODULE