FastQCFastQC Report
Thu 9 Feb 2017
SRR2727316.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727316.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2850245
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT580092.0352285505281125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT215990.7577945053846249No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT201030.7053077893303908No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144090.5055354890544497No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA77460.2717661113342888No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62450.2191039717638308No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48160.1689679308269991No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47160.1654594605025182No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA35760.1254628988034362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGT11050.021.09502211
CGGTGAT12350.018.12550514
TAAGCGT752.0686195E-417.2666664
TATACAC17100.017.20175437
CGACTAT650.001580709417.07692336
TCGGTGA13550.016.65682813
CAGTCGG14500.016.58620610
CGCTTAT3350.016.56716526
GCAGTCG14300.016.559449
GTCGGTG14100.016.53191612
ATAATAC2850.016.228073
AACGCAG220950.016.1597635
CCGCTTA3450.016.08695825
TAACCGT700.002593756415.8571426
CGCCTTA10500.015.68095225
GTGATTC14450.015.61937716
CTCTATG21750.015.5655171
TTAGGGT2400.015.4166674
GGCAGTC15950.015.0783698
GCGTGTC2109.094947E-1214.9761911
Produced by FastQC (version 0.11.5)