FastQCFastQC Report
Thu 9 Feb 2017
SRR2727315.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727315.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1906433
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT278761.4622071690953733No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT100730.5283689487120712No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT94560.4960048425515085No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72020.3777735698028727No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA43130.22623401923907108No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34600.18149077360704519No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27080.14204538003695907No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT24130.1265714556976301No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA22990.12059170188514362No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22340.11718219313241011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGT4050.021.01234611
TATACCG701.2197319E-418.55
TCGGTGA4950.018.3131313
CGGTGAT4900.017.74489814
TCGTTAC2350.016.53191423
GTCGGTG5200.016.36538712
ATACCGT1251.6592821E-716.2799996
CTTATAC26950.015.99443337
TATACTG3250.015.9384615
ATACCGA700.002593333415.8571436
CGCCTTA3650.015.71232925
CTCGTTA2900.015.31034622
CGTTACG2450.015.1020424
GTCTAGG2500.014.7999991
GTATAAG3050.014.5573771
CTATACT2550.014.5098044
GGAGTGT5650.014.407086
TAGTACT1803.3342076E-914.3888884
AACGCAG117450.014.3180075
TATACAC9950.014.31658337
Produced by FastQC (version 0.11.5)