##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727315.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1906433
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38246190660779	33.0	31.0	34.0	31.0	34.0
2	32.506665065071786	34.0	31.0	34.0	31.0	34.0
3	32.60461448159993	34.0	31.0	34.0	31.0	34.0
4	36.118600024233736	37.0	35.0	37.0	35.0	37.0
5	35.94139054454051	37.0	35.0	37.0	35.0	37.0
6	35.974894475704104	37.0	35.0	37.0	35.0	37.0
7	35.93763588859404	37.0	35.0	37.0	35.0	37.0
8	35.89783957789233	37.0	35.0	37.0	35.0	37.0
9	37.73945478283265	39.0	37.0	39.0	35.0	39.0
10	37.59032811538617	39.0	37.0	39.0	35.0	39.0
11	37.69182761733562	39.0	37.0	39.0	35.0	39.0
12	37.626204015562045	39.0	37.0	39.0	35.0	39.0
13	37.70598127497793	39.0	37.0	39.0	35.0	39.0
14	38.904399472732585	40.0	38.0	41.0	36.0	41.0
15	38.842356904229	40.0	38.0	41.0	35.0	41.0
16	38.811643000304755	40.0	38.0	41.0	35.0	41.0
17	38.77022218981732	40.0	38.0	41.0	35.0	41.0
18	38.769154226768	40.0	38.0	41.0	35.0	41.0
19	38.82374413367792	40.0	38.0	41.0	35.0	41.0
20	38.79370216524787	40.0	38.0	41.0	35.0	41.0
21	38.75401600790586	40.0	38.0	41.0	35.0	41.0
22	38.70144138293871	40.0	38.0	41.0	35.0	41.0
23	38.626891687250485	40.0	38.0	41.0	34.0	41.0
24	38.58727267100391	40.0	38.0	41.0	34.0	41.0
25	38.46873716516657	40.0	38.0	41.0	34.0	41.0
26	38.3475553559973	40.0	38.0	41.0	34.0	41.0
27	38.24245646188458	40.0	38.0	41.0	34.0	41.0
28	38.13262516962306	40.0	38.0	41.0	34.0	41.0
29	38.01309775900858	40.0	37.0	41.0	33.0	41.0
30	37.905083997182174	40.0	37.0	41.0	33.0	41.0
31	37.770073745051626	40.0	37.0	41.0	33.0	41.0
32	37.64652363864872	40.0	36.0	41.0	33.0	41.0
33	37.50768162321991	40.0	36.0	41.0	33.0	41.0
34	37.369422371517906	40.0	36.0	41.0	33.0	41.0
35	37.24918683216247	39.0	36.0	41.0	32.0	41.0
36	37.109834964040175	39.0	36.0	41.0	32.0	41.0
37	36.97866644146424	39.0	35.0	41.0	31.0	41.0
38	36.841355033195505	39.0	35.0	41.0	31.0	41.0
39	36.68662208427991	39.0	35.0	41.0	31.0	41.0
40	36.50547593332679	39.0	35.0	41.0	30.0	41.0
41	36.34037912688251	39.0	35.0	41.0	30.0	41.0
42	36.15730896391324	39.0	35.0	41.0	29.0	41.0
43	34.53529864411705	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	4.0
10	3.0
11	5.0
12	3.0
13	1.0
14	2.0
15	2.0
16	16.0
17	18.0
18	25.0
19	102.0
20	172.0
21	390.0
22	820.0
23	1452.0
24	2539.0
25	4263.0
26	6611.0
27	10246.0
28	15021.0
29	20972.0
30	27570.0
31	35183.0
32	44667.0
33	58825.0
34	79159.0
35	110431.0
36	164368.0
37	281884.0
38	500543.0
39	541135.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.40914891842514	17.187438530491235	13.003184481175053	24.400228069908568
2	15.986924271663364	22.5736230961172	36.78025925904556	24.659193373173878
3	18.802129421805013	23.953110337473177	30.932637024222725	26.312123216499085
4	13.155563295431834	15.02570507329657	34.9769438527344	36.841787778537196
5	14.446508217178364	37.908911564161976	34.1723522410701	13.472227977589563
6	34.614539299309236	35.838710303483	14.945975022463417	14.600775374744352
7	28.709637317440478	30.96799100728953	21.945119498036387	18.377252177233608
8	28.1319616267658	36.01700138426055	19.516237916569846	16.3347990724038
9	26.20417292398946	14.08698863269782	19.13715299724669	40.57168544606603
10	14.878886381005785	28.584691935147998	34.10641758719032	22.4300040966559
11	35.5784336506974	22.09839002996696	21.706034253498547	20.61714206583709
12	20.587138388813035	26.264442547941623	31.753751639842577	21.394667423402762
13	31.90424211079015	19.05343644387188	25.35090401813229	23.69141742720568
14	21.2447014922633	20.082898271274154	28.92375446711214	29.748645769350407
15	24.873258068864736	28.716141611061076	23.41828954912132	22.992310770952873
16	22.977518748364094	26.39762320522148	26.000599024460865	24.624259021953566
17	22.699407742102657	27.481532264705866	27.763839589432198	22.05522040375927
18	22.68561234514929	25.96571712722136	28.437925696838022	22.910744830791327
19	23.514752419833272	27.02025195745143	28.238443207812708	21.226552414902596
20	22.6605393423215	27.023504104261733	28.0417932337512	22.274163319665576
21	22.03219310618312	27.718152172145572	28.002400294161923	22.247254427509386
22	22.641026461459703	26.973305644625327	27.463330733364348	22.922337160550622
23	22.01755844553677	26.784051681858212	27.85657822750655	23.341811645098463
24	22.20261608983898	26.75488726852714	28.15981469057659	22.882681951057286
25	22.535016966240093	26.833410877801633	27.7326819248303	22.898890231127975
26	22.2277940006284	27.22487493659625	28.431578765159855	22.115752297615497
27	22.761985341210522	27.560475505826847	28.0176119485972	21.65992720436543
28	22.51492709158937	27.793895720437067	27.771602778592268	21.91957440938129
29	21.963898023166823	27.869796630670997	28.2900054709502	21.87629987521198
30	21.89387195878376	27.863187429088775	28.352320800154008	21.890619811973462
31	21.814509085816287	27.224717574653816	28.220031860547945	22.740741478981953
32	21.460706985244173	27.65185034040011	28.191391987024982	22.69605068733074
33	22.00129771148527	27.32091817546171	28.004550907375187	22.67323320567783
34	22.223597682163497	27.400962950179736	28.262047499177783	22.11339186847899
35	21.925868887078646	27.538077656020434	28.1556708260925	22.380382630808427
36	21.71542351606377	27.221465427843516	28.489068328129026	22.57404272796369
37	21.31824197336072	26.87164982981306	28.48933059803308	23.32077759879314
38	21.388740123571086	26.906374365110132	28.918299253107765	22.786586258211013
39	21.06420734429167	26.744606288288125	29.37055747566266	22.82062889175754
40	21.447278766156483	26.760342482531513	29.369351034104003	22.423027717208
41	21.056286793189166	26.47231767389675	29.54664548924615	22.924750043667938
42	20.518108950065383	26.74555045994273	29.46439764733405	23.271942942657834
43	20.31909854686737	26.14421802392216	29.48999518996996	24.046688239240506
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	179.0
1	324.0
2	469.0
3	2743.5
4	5018.0
5	5018.0
6	6810.0
7	8602.0
8	7848.5
9	7095.0
10	9148.5
11	11202.0
12	11202.0
13	17975.5
14	24749.0
15	38251.5
16	51754.0
17	40722.0
18	29690.0
19	29690.0
20	30874.0
21	32058.0
22	24604.0
23	17150.0
24	19520.5
25	21891.0
26	21891.0
27	25756.5
28	29622.0
29	33386.0
30	37150.0
31	40815.5
32	44481.0
33	44481.0
34	49677.5
35	54874.0
36	62112.5
37	69351.0
38	78026.0
39	86701.0
40	86701.0
41	95704.5
42	104708.0
43	113796.0
44	122884.0
45	132494.0
46	142104.0
47	142104.0
48	152136.5
49	162169.0
50	163412.5
51	164656.0
52	163948.0
53	163240.0
54	163240.0
55	149801.5
56	136363.0
57	122451.5
58	108540.0
59	98879.5
60	89219.0
61	89219.0
62	77021.5
63	64824.0
64	54242.0
65	43660.0
66	36126.5
67	28593.0
68	28593.0
69	23154.5
70	17716.0
71	14314.5
72	10913.0
73	8694.5
74	6476.0
75	6476.0
76	5091.0
77	3706.0
78	2980.5
79	2255.0
80	1722.0
81	1189.0
82	1189.0
83	897.5
84	606.0
85	476.5
86	347.0
87	245.0
88	143.0
89	143.0
90	97.0
91	51.0
92	36.5
93	22.0
94	16.5
95	11.0
96	11.0
97	6.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1906433.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.15359015397465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.3775837180535	50.154609990044996
2	9.15635447150158	11.015751883664382
3	2.813230912318669	5.076778179243282
4	1.3759535599352142	3.3107418606098102
5	0.7915775983074494	2.3808117211826945
6	0.48534195885960557	1.7517036766660763
7	0.363135062308702	1.5290714396058995
8	0.2602729378979124	1.2525081307585535
9	0.1973928149520948	1.06864978409709
>10	1.093713422225636	11.547964445859728
>50	0.05391384085169926	2.192017578591629
>100	0.026653149824745134	3.043796560721516
>500	0.003222008207793617	1.2763527273257906
>1k	0.001306219543700114	1.5387892029025036
>5k	1.7416260582668185E-4	0.8725881856600594
>10k+	1.7416260582668185E-4	1.98786463306601
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	27876	1.4622071690953733	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10073	0.5283689487120712	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9456	0.4960048425515085	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7202	0.3777735698028727	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	4313	0.22623401923907108	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3460	0.18149077360704519	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2708	0.14204538003695907	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	2413	0.1265714556976301	No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	2299	0.12059170188514362	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2234	0.11718219313241011	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.5736194243385422E-4	0.0
10	5.2453980811284736E-5	0.0	0.0	2.0981592324513895E-4	0.0
11	5.2453980811284736E-5	0.0	0.0	4.196318464902779E-4	0.0
12	5.2453980811284736E-5	0.0	0.0	7.343557313579863E-4	0.0
13	5.2453980811284736E-5	0.0	0.0	8.917176737918406E-4	0.0
14	5.2453980811284736E-5	0.0	0.0	0.0013113495202821185	0.0
15	5.2453980811284736E-5	0.0	0.0	0.001416257481904688	5.2453980811284736E-5
16	5.2453980811284736E-5	0.0	0.0	0.0020457052516401047	1.5736194243385422E-4
17	5.2453980811284736E-5	0.0	0.0	0.0027276070021868067	1.5736194243385422E-4
18	5.2453980811284736E-5	0.0	0.0	0.0030423308870545147	1.5736194243385422E-4
19	5.2453980811284736E-5	0.0	0.0	0.004720858273015626	1.5736194243385422E-4
20	5.2453980811284736E-5	0.0	0.0	0.00708128740952344	2.0981592324513895E-4
21	5.2453980811284736E-5	0.0	0.0	0.011277605874426219	2.622699040564237E-4
22	1.5736194243385422E-4	0.0	0.0	0.017204905706101396	2.622699040564237E-4
23	1.5736194243385422E-4	0.0	0.0	0.02192576397911702	2.622699040564237E-4
24	1.5736194243385422E-4	0.0	0.0	0.030056131004866156	2.622699040564237E-4
25	1.5736194243385422E-4	0.0	0.0	0.03739968831844602	2.622699040564237E-4
26	1.5736194243385422E-4	0.0	0.0	0.046002141171496716	2.622699040564237E-4
27	1.5736194243385422E-4	0.0	0.0	0.06478066630193666	2.622699040564237E-4
28	2.0981592324513895E-4	0.0	0.0	0.1835364788586853	3.1472388486770845E-4
29	2.0981592324513895E-4	0.0	0.0	0.41470117229401715	3.1472388486770845E-4
30	2.0981592324513895E-4	0.0	0.0	0.6842097257023981	3.1472388486770845E-4
31	2.0981592324513895E-4	0.0	0.0	1.2765200770234255	3.6717786567899317E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTCGGT	405	0.0	21.012346	11
TATACCG	70	1.2197319E-4	18.5	5
TCGGTGA	495	0.0	18.31313	13
CGGTGAT	490	0.0	17.744898	14
TCGTTAC	235	0.0	16.531914	23
GTCGGTG	520	0.0	16.365387	12
ATACCGT	125	1.6592821E-7	16.279999	6
CTTATAC	2695	0.0	15.994433	37
TATACTG	325	0.0	15.938461	5
ATACCGA	70	0.0025933334	15.857143	6
CGCCTTA	365	0.0	15.712329	25
CTCGTTA	290	0.0	15.310346	22
CGTTACG	245	0.0	15.10204	24
GTCTAGG	250	0.0	14.799999	1
GTATAAG	305	0.0	14.557377	1
CTATACT	255	0.0	14.509804	4
GGAGTGT	565	0.0	14.40708	6
TAGTACT	180	3.3342076E-9	14.388888	4
AACGCAG	11745	0.0	14.318007	5
TATACAC	995	0.0	14.316583	37
>>END_MODULE