FastQCFastQC Report
Thu 9 Feb 2017
SRR2727314.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727314.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4449841
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT374370.8413109592005646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT152370.3424167290471727No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT144230.324123940608215No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93380.20985019464740423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGT6350.016.89763811
AACGCAG145300.016.6156225
GGTATCA81950.015.8700421
CTTATAC48350.015.84074437
ACGCAGA163250.014.8793276
CGCAGAG166000.014.7442767
AGAGTAC171450.014.13531610
CGGTGAT7700.013.93506614
CAGAGTA175500.013.9039899
TATACCG2405.456968E-1213.8750015
GGGTAAG21300.013.8098591
GCAGAGT177700.013.7839058
CAACGCA178600.013.6212214
TCGGTGA8350.013.5149713
TCAACGC180200.013.4592123
ATCAACG179850.013.41342
TATCAAC182450.013.2729521
TATACAC19750.013.20759437
GGAGTGT16950.013.0973456
ACCCGAC20400.013.05882311
Produced by FastQC (version 0.11.5)