##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727314.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4449841
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.890368891832313	31.0	31.0	34.0	30.0	34.0
2	32.051173513840155	33.0	31.0	34.0	30.0	34.0
3	32.124163537528645	34.0	31.0	34.0	30.0	34.0
4	35.79445602663107	37.0	35.0	37.0	35.0	37.0
5	35.44290998262635	37.0	35.0	37.0	33.0	37.0
6	35.44036382423552	37.0	35.0	37.0	33.0	37.0
7	35.417222323224586	37.0	35.0	37.0	33.0	37.0
8	35.351526043290086	37.0	35.0	37.0	33.0	37.0
9	37.1396094826759	39.0	37.0	39.0	33.0	39.0
10	36.872861524715155	39.0	37.0	39.0	33.0	39.0
11	37.01567224536787	39.0	37.0	39.0	33.0	39.0
12	36.904756821648235	39.0	37.0	39.0	33.0	39.0
13	37.017974799549016	39.0	37.0	39.0	33.0	39.0
14	38.10296143165565	40.0	37.0	41.0	33.0	41.0
15	37.994600031776415	40.0	37.0	41.0	33.0	41.0
16	37.959404392201876	40.0	37.0	41.0	33.0	41.0
17	37.873184457601965	40.0	37.0	41.0	33.0	41.0
18	37.82651762164086	39.0	37.0	41.0	33.0	41.0
19	37.8773513480594	40.0	37.0	41.0	33.0	41.0
20	37.821996111771185	39.0	37.0	41.0	33.0	41.0
21	37.7702902193584	39.0	37.0	41.0	33.0	41.0
22	37.72614257453244	39.0	37.0	40.0	33.0	41.0
23	37.646601979711185	39.0	37.0	40.0	32.0	41.0
24	37.587672008954925	39.0	37.0	41.0	32.0	41.0
25	37.45825255329348	39.0	37.0	40.0	32.0	41.0
26	37.315915107978014	39.0	36.0	40.0	32.0	41.0
27	37.20601095634653	39.0	36.0	40.0	31.0	41.0
28	37.092119021780775	39.0	36.0	40.0	31.0	41.0
29	36.99323234245898	39.0	36.0	40.0	31.0	41.0
30	36.91329914934039	39.0	36.0	40.0	31.0	41.0
31	36.79896090669307	39.0	36.0	40.0	31.0	41.0
32	36.68648295523368	39.0	35.0	40.0	30.0	41.0
33	36.5743169250317	38.0	35.0	40.0	30.0	41.0
34	36.4616551467794	38.0	35.0	40.0	30.0	41.0
35	36.398531992491414	38.0	35.0	40.0	30.0	41.0
36	36.29924417524132	38.0	35.0	40.0	30.0	41.0
37	36.206243324199676	38.0	35.0	40.0	30.0	41.0
38	36.09612231088706	38.0	35.0	40.0	29.0	41.0
39	35.95577797049378	38.0	35.0	40.0	29.0	41.0
40	35.83827894075316	38.0	35.0	40.0	28.0	41.0
41	35.7140230403738	38.0	35.0	40.0	28.0	41.0
42	35.60060033605695	38.0	34.0	40.0	27.0	41.0
43	33.90651171581187	37.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	15.0
11	13.0
12	10.0
13	15.0
14	20.0
15	19.0
16	54.0
17	103.0
18	227.0
19	452.0
20	1009.0
21	2038.0
22	3769.0
23	6584.0
24	10617.0
25	16587.0
26	24926.0
27	35660.0
28	50440.0
29	68649.0
30	89618.0
31	116082.0
32	148579.0
33	192401.0
34	256120.0
35	353167.0
36	519293.0
37	811268.0
38	1186943.0
39	555156.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.7002223225504	18.409242038086305	13.272024775716707	25.618510863646588
2	16.973033418497423	20.893690358824056	37.12582090011756	25.00745532256096
3	18.43854645592955	22.512579662958743	31.360333998450727	27.68853988266098
4	13.742873059958772	15.584781568599867	35.427175937297534	35.245169434143826
5	13.909710481790249	37.71220140225235	34.83924481796091	13.53884329799649
6	34.51352531472473	36.757425714761496	14.926398493788879	13.802650476724898
7	29.123400139465655	31.619938779835056	21.14612634473906	18.11053473596023
8	27.370124011172535	36.795988890389566	19.001330609340872	16.832556489097026
9	26.893657548662976	13.536416244984933	18.469963308801372	41.099962897550725
10	14.726166620335423	28.13736490809447	34.679486300746476	22.45698217082363
11	37.06689744644809	21.201723836874173	21.894827253378267	19.836551463299475
12	20.890386870002768	25.15716853703312	31.673289000663168	22.27915559230094
13	31.36833428430364	19.449885962217525	24.93489093205802	24.24688882142081
14	21.330357646486693	19.607150008281195	27.606941461503904	31.455550883728208
15	24.831493979223076	28.550413374320566	22.650022776094698	23.96806987036166
16	23.78884998362863	26.83179016958134	24.91835101523852	24.46100883155151
17	23.560841836820686	26.79309215767485	26.254263916396113	23.391802089108353
18	23.575943500003707	26.201790131377727	26.96882877388203	23.253437594736532
19	24.65910579726332	26.0026594208647	26.982829274124626	22.355405507747356
20	23.867706733791163	25.719638072461464	27.020111505107707	23.392543688639662
21	23.470366694000976	26.04261590470311	27.369629611484996	23.117387789810916
22	24.070635332813016	25.327129665981325	26.755180690725805	23.847054310479855
23	23.323192896105727	25.645747791887395	27.243108236901048	23.78795107510583
24	23.31317006607652	25.442189956899586	27.360325908274024	23.884314068749873
25	23.750489062418183	25.353962085386872	27.066630021162553	23.82891883103239
26	23.393240342744832	25.666917087599312	27.369112739084382	23.570729830571473
27	23.869864114245882	26.053739897672752	26.795159647277284	23.28123634080409
28	23.546684027586604	26.352020218250495	26.540161772072306	23.561133982090595
29	23.346160008863237	26.397549934930257	27.03060626211139	23.225683794095115
30	23.912629687218036	26.08740402185157	26.62946833381238	23.37049795711802
31	23.58365164058671	25.6500850255099	27.093012986306704	23.67325034759669
32	23.078442578060653	26.1343045740286	27.048494541715083	23.73875830619566
33	23.288337718134198	25.52700197602566	27.119171224320148	24.065489081519992
34	23.52762716690327	25.615566938234423	27.608312297001174	23.248493597861135
35	23.45038845208177	25.52145121589738	27.23598438685787	23.792175945162985
36	23.174558371860925	25.27977965954289	27.70665288939537	23.839009079200807
37	22.970146573776457	25.10226769900318	27.7831949501117	24.144390777108665
38	23.054733865771833	24.943790126433733	27.964594690012518	24.036881317781916
39	22.618246359813753	24.964240295327407	28.54596377713271	23.871549567726127
40	23.08338657493605	24.803470506024823	28.423060509353032	23.6900824096861
41	22.4191381220138	24.835224449592694	28.71046403680491	24.0351733915886
42	22.051124972779927	25.06231121516477	28.593268838145004	24.293294973910303
43	21.748395055014324	24.60863208370816	28.482927816971436	25.160045044306077
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1649.0
1	1779.5
2	1910.0
3	4591.0
4	7272.0
5	7272.0
6	8794.5
7	10317.0
8	9550.5
9	8784.0
10	11584.5
11	14385.0
12	14385.0
13	21395.5
14	28406.0
15	45836.0
16	63266.0
17	51233.5
18	39201.0
19	39201.0
20	42539.0
21	45877.0
22	39573.0
23	33269.0
24	39002.5
25	44736.0
26	44736.0
27	53640.5
28	62545.0
29	71117.5
30	79690.0
31	91984.5
32	104279.0
33	104279.0
34	119008.0
35	133737.0
36	152632.5
37	171528.0
38	192247.0
39	212966.0
40	212966.0
41	235728.0
42	258490.0
43	280314.0
44	302138.0
45	326884.5
46	351631.0
47	351631.0
48	370402.0
49	389173.0
50	393663.0
51	398153.0
52	384636.5
53	371120.0
54	371120.0
55	350833.5
56	330547.0
57	304987.0
58	279427.0
59	255507.0
60	231587.0
61	231587.0
62	200659.5
63	169732.0
64	141893.5
65	114055.0
66	94001.0
67	73947.0
68	73947.0
69	60134.0
70	46321.0
71	37396.0
72	28471.0
73	23113.0
74	17755.0
75	17755.0
76	14116.0
77	10477.0
78	8320.5
79	6164.0
80	4809.0
81	3454.0
82	3454.0
83	2606.0
84	1758.0
85	1336.5
86	915.0
87	685.5
88	456.0
89	456.0
90	305.5
91	155.0
92	107.5
93	60.0
94	42.0
95	24.0
96	24.0
97	16.0
98	8.0
99	7.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4449841.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.86530500261386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.07969232877613	42.5965174680239
2	10.663116687122905	11.487440652606734
3	3.732424794194204	6.031445998157672
4	1.8000663117004754	3.878444836187052
5	1.0611461968730422	2.857948177346507
6	0.6902305619040674	2.2307687843452886
7	0.4898220868130811	1.8469091272242393
8	0.3832249665497585	1.6514023766255388
9	0.3034518932753221	1.471097590640627
>10	1.6593722803000632	16.5592069569905
>50	0.1002738567453463	3.6613353258005246
>100	0.03555176942393614	3.1975248116769204
>500	0.0012092750864960092	0.44482491707269434
>1k	2.5019474106232123E-4	0.36829892094127653
>5k	4.169912351038014E-5	0.20974418026159797
>10k+	1.2509737053114042E-4	1.5070898760989975
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	37437	0.8413109592005646	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15237	0.3424167290471727	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	14423	0.324123940608215	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9338	0.20985019464740423	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.2472713069972614E-5	0.0	0.0	0.0	0.0
6	2.2472713069972614E-5	0.0	0.0	0.0	0.0
7	2.2472713069972614E-5	0.0	0.0	0.0	0.0
8	2.2472713069972614E-5	0.0	0.0	0.0	0.0
9	2.2472713069972614E-5	0.0	0.0	2.2472713069972614E-5	0.0
10	2.2472713069972614E-5	0.0	0.0	4.494542613994523E-5	0.0
11	2.2472713069972614E-5	0.0	0.0	2.471998437696987E-4	0.0
12	2.2472713069972614E-5	0.0	0.0	3.146179829796166E-4	0.0
13	2.2472713069972614E-5	0.0	0.0	3.595634091195618E-4	2.2472713069972614E-5
14	2.2472713069972614E-5	0.0	0.0	3.595634091195618E-4	6.741813920991784E-5
15	2.2472713069972614E-5	0.0	0.0	4.943996875393975E-4	1.573089914898083E-4
16	4.494542613994523E-5	0.0	0.0	7.640722443790688E-4	2.0225441762975353E-4
17	4.494542613994523E-5	0.0	0.0	9.663266620088223E-4	2.0225441762975353E-4
18	4.494542613994523E-5	0.0	0.0	0.0010786902273586855	2.0225441762975353E-4
19	4.494542613994523E-5	2.2472713069972614E-5	0.0	0.001280944644988439	2.0225441762975353E-4
20	6.741813920991784E-5	2.2472713069972614E-5	0.0	0.00206748960243748	2.471998437696987E-4
21	8.989085227989046E-5	2.2472713069972614E-5	0.0	0.0031237071167261933	2.6967255683967137E-4
22	1.1236356534986306E-4	2.2472713069972614E-5	0.0	0.0054608692760033445	2.6967255683967137E-4
23	1.1236356534986306E-4	2.2472713069972614E-5	0.0	0.006854177486341647	3.820361221895344E-4
24	1.3483627841983568E-4	2.2472713069972614E-5	0.0	0.008989085227989045	3.820361221895344E-4
25	1.3483627841983568E-4	2.2472713069972614E-5	0.0	0.010247557159907512	3.820361221895344E-4
26	1.3483627841983568E-4	2.2472713069972614E-5	0.0	0.01195548335322543	3.820361221895344E-4
27	1.3483627841983568E-4	2.2472713069972614E-5	0.0	0.025618892899768778	4.0450883525950705E-4
28	1.3483627841983568E-4	2.2472713069972614E-5	0.0	0.12231897723986093	4.0450883525950705E-4
29	1.3483627841983568E-4	2.2472713069972614E-5	0.0	0.28877436294914804	4.0450883525950705E-4
30	1.573089914898083E-4	2.2472713069972614E-5	0.0	0.4862420926949974	4.0450883525950705E-4
31	1.573089914898083E-4	2.2472713069972614E-5	0.0	0.9718549494240356	4.2698154832947964E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTCGGT	635	0.0	16.897638	11
AACGCAG	14530	0.0	16.615622	5
GGTATCA	8195	0.0	15.870042	1
CTTATAC	4835	0.0	15.840744	37
ACGCAGA	16325	0.0	14.879327	6
CGCAGAG	16600	0.0	14.744276	7
AGAGTAC	17145	0.0	14.135316	10
CGGTGAT	770	0.0	13.935066	14
CAGAGTA	17550	0.0	13.903989	9
TATACCG	240	5.456968E-12	13.875001	5
GGGTAAG	2130	0.0	13.809859	1
GCAGAGT	17770	0.0	13.783905	8
CAACGCA	17860	0.0	13.621221	4
TCGGTGA	835	0.0	13.51497	13
TCAACGC	18020	0.0	13.459212	3
ATCAACG	17985	0.0	13.4134	2
TATCAAC	18245	0.0	13.272952	1
TATACAC	1975	0.0	13.207594	37
GGAGTGT	1695	0.0	13.097345	6
ACCCGAC	2040	0.0	13.058823	11
>>END_MODULE