FastQCFastQC Report
Thu 9 Feb 2017
SRR2727313.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727313.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2920250
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT490871.6809177296464344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT180990.6197757041349199No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT168790.5779984590360414No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123480.4228405102302885No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA65900.22566561082099135No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50090.17152641041006764No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37280.1276603030562452No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36560.12519476072254088No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA35970.12317438575464429No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG500.00703741618.4999984
AGTCGGT6300.017.91269911
CGGTGAT6400.017.3437514
TATACCG1502.5283953E-1017.2666665
CTTATAC27700.016.2292437
TTATGCG803.384798E-416.18754
TCGGTGA7600.015.82236913
GTCGGTG7650.014.993463512
CTATACT4350.014.88505654
AACGCAG184900.014.75797755
TAGGGTG4300.014.6279085
TAGTACT2800.014.5357144
ATACCGA1152.2120395E-514.4782616
TATACTG5650.014.407085
CTAGGAC4400.014.2954543
GACCGTG3850.013.9350647
TAGCGTG800.006301685213.8750015
GTATAGG4350.013.6091951
GTACCGT1652.5990084E-713.4545456
GTGATTC8400.013.43452416
Produced by FastQC (version 0.11.5)