##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727313.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2920250
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31680232856776	33.0	31.0	34.0	30.0	34.0
2	32.44406917215992	34.0	31.0	34.0	31.0	34.0
3	32.53923191507577	34.0	31.0	34.0	31.0	34.0
4	36.073326941186544	37.0	35.0	37.0	35.0	37.0
5	35.8931992123962	37.0	35.0	37.0	35.0	37.0
6	35.92493759095968	37.0	35.0	37.0	35.0	37.0
7	35.88743361013612	37.0	35.0	37.0	35.0	37.0
8	35.84728704734184	37.0	35.0	37.0	35.0	37.0
9	37.678128242445	39.0	37.0	39.0	35.0	39.0
10	37.520355449019775	39.0	37.0	39.0	35.0	39.0
11	37.62973067374369	39.0	37.0	39.0	35.0	39.0
12	37.55490043660645	39.0	37.0	39.0	35.0	39.0
13	37.63729475216163	39.0	37.0	39.0	35.0	39.0
14	38.80772057186885	40.0	38.0	41.0	35.0	41.0
15	38.740221898810034	40.0	38.0	41.0	35.0	41.0
16	38.7183333618697	40.0	38.0	41.0	35.0	41.0
17	38.682903518534374	40.0	38.0	41.0	35.0	41.0
18	38.68178375139115	40.0	38.0	41.0	35.0	41.0
19	38.748830065919016	40.0	38.0	41.0	35.0	41.0
20	38.720205119424705	40.0	38.0	41.0	35.0	41.0
21	38.677818679907546	40.0	38.0	41.0	34.0	41.0
22	38.632611591473335	40.0	38.0	41.0	34.0	41.0
23	38.56163616128756	40.0	38.0	41.0	34.0	41.0
24	38.51840356133893	40.0	38.0	41.0	34.0	41.0
25	38.40318089204691	40.0	38.0	41.0	34.0	41.0
26	38.28509853608424	40.0	38.0	41.0	34.0	41.0
27	38.18194058727849	40.0	38.0	41.0	34.0	41.0
28	38.07782141939902	40.0	37.0	41.0	33.0	41.0
29	37.95269754301858	40.0	37.0	41.0	33.0	41.0
30	37.850805239277456	40.0	37.0	41.0	33.0	41.0
31	37.708655080900606	40.0	37.0	41.0	33.0	41.0
32	37.58777570413492	40.0	36.0	41.0	33.0	41.0
33	37.4572536597894	40.0	36.0	41.0	33.0	41.0
34	37.32473726564506	39.0	36.0	41.0	32.0	41.0
35	37.20912353394401	39.0	36.0	41.0	32.0	41.0
36	37.083311017892306	39.0	35.0	41.0	31.0	41.0
37	36.95342487800702	39.0	35.0	41.0	31.0	41.0
38	36.8184052735211	39.0	35.0	41.0	31.0	41.0
39	36.66949678965842	39.0	35.0	41.0	31.0	41.0
40	36.49979179864738	39.0	35.0	41.0	30.0	41.0
41	36.34167862340553	39.0	35.0	41.0	30.0	41.0
42	36.160009245783755	39.0	35.0	40.0	29.0	41.0
43	34.561459806523416	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	25.0
11	13.0
12	22.0
13	11.0
14	15.0
15	9.0
16	16.0
17	38.0
18	59.0
19	149.0
20	337.0
21	668.0
22	1256.0
23	2409.0
24	4023.0
25	6653.0
26	10589.0
27	16051.0
28	23653.0
29	33027.0
30	43334.0
31	56141.0
32	71260.0
33	93491.0
34	125444.0
35	172762.0
36	256476.0
37	437051.0
38	760148.0
39	805116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.15589418714151	17.356561938190225	13.01306395000428	24.47447992466398
2	16.049653283109322	22.57015666466912	36.68935878777502	24.690831264446537
3	18.76222926119339	23.674377193733413	31.358342607653455	26.205050937419745
4	12.745312901292696	14.97464258197072	35.466792226692924	36.813252290043664
5	14.076637274205975	38.51824330108723	34.25535484975602	13.149764574950776
6	34.41544388322917	36.53872099991439	14.723020289358788	14.322814827497647
7	28.86335074051879	31.261467340124987	21.564763290814142	18.310418628542077
8	28.570190908312643	36.09379333961134	19.031658248437633	16.304357503638386
9	26.474411437376936	13.706840167793855	18.87389778272408	40.94485061210513
10	14.583443198356305	28.981320092457835	34.241931341494734	22.19330536769112
11	35.94394315555175	22.045509802242957	21.670541905658762	20.34000513654653
12	20.698330622378222	26.456638986388153	31.798750107011386	21.04628028422224
13	32.10536769112233	19.167126102217278	25.30177210855235	23.425734098108038
14	21.197123533944012	20.073418371714748	29.159900693433784	29.56955740090746
15	24.686345347144936	29.026932625631368	23.54699083982536	22.73973118739834
16	22.783631538395685	26.54673401249893	25.99404160602688	24.675592843078505
17	22.670832976628713	27.28170533344748	27.951545244414007	22.095916445509804
18	22.55988357161202	25.7720058214194	28.835236709185857	22.832873897782726
19	23.400188340039378	26.612687269925523	28.73897782724082	21.248146562794282
20	22.59618183374711	26.61457067031932	28.60344148617413	22.18580600975944
21	22.109168735553464	27.163188083212052	28.614604913962843	22.113038267271637
22	22.54217960791028	26.61326941186542	28.028011300402362	22.816539679821936
23	22.014142624775275	26.51615443883229	28.349798818594294	23.119904117798136
24	22.082047769882713	26.44965328310932	28.69521445081757	22.773084496190393
25	22.392192449276603	26.451160003424363	28.404520160945125	22.75212738635391
26	22.109750877493365	26.78592586251177	28.925160517079018	22.179162742915846
27	22.598373426932625	27.18667922266929	28.477459121650543	21.73748822874754
28	22.3156579060012	27.39046314527866	28.4251690779899	21.868709870730246
29	21.909870730245697	27.52778015580858	28.752195873640957	21.810153240304768
30	21.86713466312816	27.606574779556546	28.67768170533345	21.84860885198185
31	21.766218645663898	27.076996832462974	28.603099049738894	22.553685472134237
32	21.35224723910624	27.55503809605342	28.5835801729304	22.509134491909936
33	21.911035014125503	27.1632565704991	28.449105384812945	22.476603030562455
34	22.21241332077733	27.13517678280969	28.663573324201696	21.988836572211284
35	21.90309048882801	27.391764403732555	28.558822018662788	22.146323088776647
36	21.740433182090573	27.02861056416403	28.766167280198612	22.464788973546785
37	21.36864994435408	26.820683160688297	28.799452101703622	23.011214793254002
38	21.422891875695573	26.768804040749938	29.206300830408356	22.602003253146137
39	21.238866535399367	26.613885797448848	29.492029791969866	22.65521787518192
40	21.44244499614759	26.573649516308535	29.690505949833064	22.293399537710812
41	21.07939388750963	26.305761493022857	29.853163256570497	22.76168136289701
42	20.55234997003681	26.525776902662447	29.88114031332934	23.040732813971406
43	20.33134149473504	26.02742915846246	29.95421624860885	23.68701309819365
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	297.0
1	482.5
2	668.0
3	3717.0
4	6766.0
5	6766.0
6	9228.5
7	11691.0
8	10545.5
9	9400.0
10	12311.5
11	15223.0
12	15223.0
13	25285.5
14	35348.0
15	58705.5
16	82063.0
17	63832.0
18	45601.0
19	45601.0
20	48778.0
21	51955.0
22	40101.0
23	28247.0
24	32844.5
25	37442.0
26	37442.0
27	44011.5
28	50581.0
29	57092.5
30	63604.0
31	69140.0
32	74676.0
33	74676.0
34	82460.0
35	90244.0
36	100672.5
37	111101.0
38	123718.5
39	136336.0
40	136336.0
41	148768.5
42	161201.0
43	173518.0
44	185835.0
45	198623.5
46	211412.0
47	211412.0
48	225062.0
49	238712.0
50	241862.0
51	245012.0
52	241826.0
53	238640.0
54	238640.0
55	221561.0
56	204482.0
57	184213.0
58	163944.0
59	149978.5
60	136013.0
61	136013.0
62	118499.0
63	100985.0
64	84296.5
65	67608.0
66	56121.0
67	44634.0
68	44634.0
69	36386.5
70	28139.0
71	22748.0
72	17357.0
73	14022.5
74	10688.0
75	10688.0
76	8505.5
77	6323.0
78	5020.5
79	3718.0
80	2912.5
81	2107.0
82	2107.0
83	1630.5
84	1154.0
85	886.5
86	619.0
87	444.5
88	270.0
89	270.0
90	180.0
91	90.0
92	62.5
93	35.0
94	28.0
95	21.0
96	21.0
97	12.0
98	3.0
99	4.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2920250.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.6317840817909
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.93002934485028	48.62335574443845
2	9.486101440867486	11.123741029178161
3	2.892973005549091	5.088605058474119
4	1.3252384430584785	3.108043770011738
5	0.7957319432800054	2.3327591742688583
6	0.5023283605310725	1.7671444783690715
7	0.3610902572212926	1.4819956196806015
8	0.24800848716908758	1.163294405611964
9	0.2089124951263669	1.1024021075613604
>10	1.1493763588722694	12.165507289929586
>50	0.06558204720916888	2.620801757028159
>100	0.030147253025140573	3.21760192455265
>500	0.002909456649574273	1.1958464221663694
>1k	0.0012219717927639242	1.323820514999381
>5k	1.1637826597751658E-4	0.3957268737043921
>10k+	2.3275653195503317E-4	3.289353830025136
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	49087	1.6809177296464344	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18099	0.6197757041349199	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16879	0.5779984590360414	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12348	0.4228405102302885	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	6590	0.22566561082099135	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5009	0.17152641041006764	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3728	0.1276603030562452	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3656	0.12519476072254088	No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	3597	0.12317438575464429	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.424364352367092E-5
2	0.0	0.0	0.0	0.0	6.848728704734184E-5
3	0.0	0.0	0.0	0.0	6.848728704734184E-5
4	0.0	0.0	0.0	0.0	6.848728704734184E-5
5	0.0	0.0	0.0	0.0	6.848728704734184E-5
6	3.424364352367092E-5	0.0	0.0	0.0	6.848728704734184E-5
7	3.424364352367092E-5	0.0	0.0	3.424364352367092E-5	6.848728704734184E-5
8	3.424364352367092E-5	0.0	0.0	3.424364352367092E-5	6.848728704734184E-5
9	6.848728704734184E-5	0.0	0.0	1.0273093057101276E-4	6.848728704734184E-5
10	2.0546186114202552E-4	0.0	0.0	1.3697457409468368E-4	6.848728704734184E-5
11	2.0546186114202552E-4	0.0	0.0	1.712182176183546E-4	6.848728704734184E-5
12	2.3970550466569644E-4	0.0	0.0	2.7394914818936736E-4	1.0273093057101276E-4
13	2.3970550466569644E-4	6.848728704734184E-5	0.0	3.081927917130383E-4	1.3697457409468368E-4
14	3.081927917130383E-4	6.848728704734184E-5	0.0	4.4516736580772195E-4	2.0546186114202552E-4
15	3.081927917130383E-4	1.0273093057101276E-4	0.0	7.533601575207602E-4	2.3970550466569644E-4
16	3.081927917130383E-4	1.0273093057101276E-4	0.0	0.0011985275233284821	2.7394914818936736E-4
17	3.081927917130383E-4	1.0273093057101276E-4	0.0	0.0016436948891362041	2.7394914818936736E-4
18	3.081927917130383E-4	1.0273093057101276E-4	0.0	0.0022258368290386096	3.766800787603801E-4
19	3.766800787603801E-4	1.0273093057101276E-4	0.0	0.0036298262135091176	3.766800787603801E-4
20	3.766800787603801E-4	1.0273093057101276E-4	0.0	0.0053077647461689925	3.766800787603801E-4
21	3.766800787603801E-4	1.0273093057101276E-4	0.0	0.007704819792825957	3.766800787603801E-4
22	3.766800787603801E-4	1.0273093057101276E-4	0.0	0.011779813372142796	3.766800787603801E-4
23	3.766800787603801E-4	1.712182176183546E-4	0.0	0.01455354849756014	4.4516736580772195E-4
24	4.1092372228405103E-4	1.712182176183546E-4	0.0	0.02016950603544217	4.4516736580772195E-4
25	4.4516736580772195E-4	1.712182176183546E-4	0.0	0.023319921239619897	4.4516736580772195E-4
26	4.4516736580772195E-4	1.712182176183546E-4	0.0	0.028490711411694205	4.7941100933139287E-4
27	4.4516736580772195E-4	1.712182176183546E-4	0.0	0.038284393459464085	5.136546528550637E-4
28	4.4516736580772195E-4	1.712182176183546E-4	0.0	0.0985532060611249	5.136546528550637E-4
29	4.4516736580772195E-4	1.712182176183546E-4	0.0	0.2352538310076192	5.136546528550637E-4
30	4.4516736580772195E-4	1.712182176183546E-4	0.0	0.3941100933139286	5.136546528550637E-4
31	4.4516736580772195E-4	1.712182176183546E-4	0.0	0.8088006163855834	5.136546528550637E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATATCG	50	0.007037416	18.499998	4
AGTCGGT	630	0.0	17.912699	11
CGGTGAT	640	0.0	17.34375	14
TATACCG	150	2.5283953E-10	17.266666	5
CTTATAC	2770	0.0	16.22924	37
TTATGCG	80	3.384798E-4	16.1875	4
TCGGTGA	760	0.0	15.822369	13
GTCGGTG	765	0.0	14.9934635	12
CTATACT	435	0.0	14.8850565	4
AACGCAG	18490	0.0	14.7579775	5
TAGGGTG	430	0.0	14.627908	5
TAGTACT	280	0.0	14.535714	4
ATACCGA	115	2.2120395E-5	14.478261	6
TATACTG	565	0.0	14.40708	5
CTAGGAC	440	0.0	14.295454	3
GACCGTG	385	0.0	13.935064	7
TAGCGTG	80	0.0063016852	13.875001	5
GTATAGG	435	0.0	13.609195	1
GTACCGT	165	2.5990084E-7	13.454545	6
GTGATTC	840	0.0	13.434524	16
>>END_MODULE