FastQCFastQC Report
Thu 9 Feb 2017
SRR2727312.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727312.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2704485
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT164690.608951426981477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT64190.23734648186253576No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT57030.21087194049883803No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39620.14649739229465128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG1200.023.1250021
CTTATAC19650.017.04071237
ATACCGT1107.817216E-716.8181826
CCCCTAT2450.015.8571431
CCCTATA2006.184564E-1114.7999992
GTTACGG3900.014.70512925
CGGTGAT3150.014.68253914
CTTACAC5700.014.6052633
GTCTTAG4650.014.322581
CTATAGT2101.364242E-1014.0952384
AACGCAG76500.014.0261435
TCTAACG1203.3036056E-513.8750012
CGTATAC800.006301527413.8753
GTCTAAC2950.013.796611
CCTATAC3100.013.7258063
ACTAGAC2700.013.7037043
CGGGCGT1254.849121E-513.326
CTGTGCG5400.013.0185189
ATAATAC3450.012.8695653
GAGTACT51550.012.77594511
Produced by FastQC (version 0.11.5)