##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2727312.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2704485 Sequences flagged as poor quality 0 Sequence length 43 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.31391854641457 33.0 31.0 34.0 30.0 34.0 2 32.466217412927044 34.0 31.0 34.0 31.0 34.0 3 32.56436253112885 34.0 31.0 34.0 31.0 34.0 4 36.10325329961157 37.0 35.0 37.0 35.0 37.0 5 35.906347788950576 37.0 35.0 37.0 35.0 37.0 6 35.91374882833515 37.0 35.0 37.0 35.0 37.0 7 35.881618496682364 37.0 35.0 37.0 35.0 37.0 8 35.832271208751386 37.0 35.0 37.0 35.0 37.0 9 37.66994677360015 39.0 37.0 39.0 35.0 39.0 10 37.49766961177451 39.0 37.0 39.0 35.0 39.0 11 37.601307457796956 39.0 37.0 39.0 35.0 39.0 12 37.525426837272164 39.0 37.0 39.0 35.0 39.0 13 37.60063930840807 39.0 37.0 39.0 35.0 39.0 14 38.8408743254261 40.0 38.0 41.0 36.0 41.0 15 38.76195541849927 40.0 38.0 41.0 35.0 41.0 16 38.72906708670967 40.0 38.0 41.0 35.0 41.0 17 38.66236714198821 40.0 38.0 41.0 35.0 41.0 18 38.62792324601541 40.0 38.0 41.0 34.0 41.0 19 38.68116776391808 40.0 38.0 41.0 34.0 41.0 20 38.651986237675565 40.0 38.0 41.0 34.0 41.0 21 38.61346282194207 40.0 38.0 41.0 34.0 41.0 22 38.58470355723918 40.0 38.0 41.0 34.0 41.0 23 38.52116391845397 40.0 38.0 41.0 34.0 41.0 24 38.48056912868809 40.0 38.0 41.0 34.0 41.0 25 38.370743783012294 40.0 38.0 41.0 34.0 41.0 26 38.270022203857664 40.0 38.0 41.0 34.0 41.0 27 38.18602247747723 40.0 38.0 41.0 34.0 41.0 28 38.1046376666907 40.0 38.0 41.0 33.0 41.0 29 38.02691492095538 40.0 38.0 41.0 33.0 41.0 30 37.96714309748437 40.0 37.0 41.0 33.0 41.0 31 37.87776526769422 40.0 37.0 41.0 33.0 41.0 32 37.79385687108636 40.0 37.0 41.0 33.0 41.0 33 37.70613480939994 40.0 37.0 41.0 33.0 41.0 34 37.62066899982806 40.0 37.0 41.0 33.0 41.0 35 37.561795683836294 40.0 37.0 41.0 33.0 41.0 36 37.481273883937234 40.0 37.0 41.0 32.0 41.0 37 37.39831945823327 40.0 37.0 41.0 32.0 41.0 38 37.30769665943794 40.0 36.0 41.0 32.0 41.0 39 37.205347043891905 39.0 36.0 41.0 31.0 41.0 40 37.09838065287846 39.0 36.0 41.0 31.0 41.0 41 36.99281933528934 39.0 36.0 41.0 31.0 41.0 42 36.889999759658494 39.0 36.0 41.0 31.0 41.0 43 35.21050847018933 38.0 34.0 40.0 27.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 1.0 11 3.0 12 1.0 13 1.0 14 4.0 15 3.0 16 10.0 17 29.0 18 51.0 19 124.0 20 271.0 21 589.0 22 1116.0 23 2020.0 24 3424.0 25 5550.0 26 8681.0 27 12974.0 28 18733.0 29 26438.0 30 35870.0 31 47558.0 32 62238.0 33 81950.0 34 111500.0 35 153934.0 36 227586.0 37 379572.0 38 743758.0 39 780494.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.23773842339669 17.401390652933923 11.977881186251727 26.38298973741766 2 16.90122148948876 20.29329058952074 36.57942269970068 26.22606522128982 3 18.326372673540433 21.862424823949848 31.056596727288188 28.75460577522153 4 13.322573428952278 14.789728913268146 35.4518512766756 36.43584638110398 5 14.277283845168304 37.16341558559208 34.86800629324991 13.6912942759897 6 35.68538927004587 35.94732453683419 14.62230332207426 13.74498287104569 7 30.266390828568102 31.103925516318263 20.565320199594378 18.06436345551926 8 28.19945387014533 35.9903641543584 18.66836014989915 17.14182182559711 9 27.127013091216995 13.26444775992472 17.942972506780404 41.665566642077884 10 14.682055918224727 27.69736197464582 34.560184286472285 23.060397820657165 11 37.59932112768235 20.73914257243061 21.539553741285307 20.12198255860173 12 21.25754071477564 24.687768650963122 31.289617062028448 22.76507357223279 13 31.880820193123643 18.815338225207388 24.473457978136317 24.83038360353265 14 21.922251371333175 19.09764705664849 26.824885329369547 32.15521624264879 15 25.479897281737557 27.783404234077835 22.11626243074005 24.620436053444557 16 24.614519954815798 26.12453017857374 24.3788373756926 24.882112490917866 17 24.10836074150901 26.3918638853608 25.741721621676582 23.758053751453602 18 24.143672455199418 25.550298855419797 26.35522104947892 23.950807639901868 19 25.078046282379084 25.7206085446952 26.14645671911658 23.054888453809134 20 24.440771533212423 25.41862868531347 26.07098948598347 24.06961029549064 21 24.06177146480753 25.772004651532548 26.184874384587083 23.981349499072838 22 24.633747275359262 25.178102300437978 25.73521391318495 24.45293651101781 23 24.103332057674567 25.194445522899922 25.991602837508804 24.710619581916703 24 24.055892341795204 25.10322667716774 26.19589311828315 24.644987862753904 25 24.436186556775137 24.940275135561855 26.010090645723675 24.613447661939333 26 24.10995069301549 25.26935812178659 26.335845826469733 24.284845358728184 27 24.514537887989764 25.652795264163046 25.90038399177662 23.932282856070564 28 24.328106829950986 25.890067794792728 25.693764247167206 24.08806112808908 29 23.965413008391618 26.048323433112035 26.089329391732623 23.896934166763728 30 24.24202759490254 25.863075594799014 25.834974126312403 24.059922683986045 31 24.089725030828422 25.35096330724704 26.0692886076277 24.490023054296845 32 23.594140843820544 25.902935309310276 26.036676113936664 24.466247732932516 33 23.937829198535027 25.440888006404176 26.030870942157197 24.5904118529036 34 24.267207989691197 25.373148677104883 26.501311709992848 23.858331623211075 35 24.03932726563468 25.497016992144527 26.279088255250077 24.184567486970717 36 23.86605952704489 25.181688935231662 26.52863669053443 24.42361484718902 37 23.64546299942503 24.87116770845466 26.627842269415435 24.855527022704877 38 23.63492494874255 24.83027267668336 26.837715868270667 24.697086506303418 39 23.29670898525967 24.584939461672 27.440159586760508 24.678191966307818 40 23.690943007633617 24.45293651101781 27.56240097467725 24.293719506671327 41 23.189294819531263 24.293312774890598 27.91655342884135 24.600838976736792 42 22.690752583209004 24.423097188559005 28.0211574477211 24.864992780510892 43 22.47008210435628 23.962750764008675 28.096402827155632 25.47076430447941 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 81.0 1 131.0 2 181.0 3 1047.0 4 1913.0 5 1913.0 6 2593.0 7 3273.0 8 3007.0 9 2741.0 10 3692.5 11 4644.0 12 4644.0 13 7897.5 14 11151.0 15 19364.0 16 27577.0 17 21926.5 18 16276.0 19 16276.0 20 18414.5 21 20553.0 22 18082.0 23 15611.0 24 19259.0 25 22907.0 26 22907.0 27 28273.0 28 33639.0 29 39373.0 30 45107.0 31 52118.5 32 59130.0 33 59130.0 34 67927.0 35 76724.0 36 88098.5 37 99473.0 38 112544.5 39 125616.0 40 125616.0 41 140144.0 42 154672.0 43 168777.0 44 182882.0 45 197141.5 46 211401.0 47 211401.0 48 224554.5 49 237708.0 50 242256.0 51 246804.0 52 243383.0 53 239962.0 54 239962.0 55 225913.5 56 211865.0 57 195588.0 58 179311.0 59 164249.5 60 149188.0 61 149188.0 62 130590.5 63 111993.0 64 94474.5 65 76956.0 66 63986.0 67 51016.0 68 51016.0 69 41912.0 70 32808.0 71 26971.5 72 21135.0 73 17000.0 74 12865.0 75 12865.0 76 10352.5 77 7840.0 78 6196.0 79 4552.0 80 3521.0 81 2490.0 82 2490.0 83 1900.5 84 1311.0 85 985.5 86 660.0 87 480.0 88 300.0 89 300.0 90 203.0 91 106.0 92 72.0 93 38.0 94 26.5 95 15.0 96 15.0 97 10.5 98 6.0 99 5.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 2704485.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.296186670685714 #Duplication Level Percentage of deduplicated Percentage of total 1 81.10269746778918 48.090806885461774 2 10.04402381767773 11.911446224356641 3 3.3324358523980497 5.928022151156412 4 1.550029079445315 3.6764325455912217 5 0.9246367489327214 2.741371663364531 6 0.6058200973054029 2.1553692947204492 7 0.42514662983629203 1.7646701747629983 8 0.32904714463943485 1.5608992729596842 9 0.23733846964180774 1.2665939580013956 >10 1.360013015423327 14.424289047776062 >50 0.06524267188179153 2.606406705635434 >100 0.02232047127399552 2.1246555857825897 >500 6.866935652613331E-4 0.29017348251366815 >1k 3.745601264939427E-4 0.40052010214564804 >5k 1.248533754979809E-4 0.44871577432620563 >10k+ 6.242668774899045E-5 0.6096271314451642 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 16469 0.608951426981477 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 6419 0.23734648186253576 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 5703 0.21087194049883803 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3962 0.14649739229465128 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.697561642974541E-5 2 0.0 0.0 0.0 0.0 3.697561642974541E-5 3 0.0 0.0 0.0 0.0 3.697561642974541E-5 4 0.0 0.0 0.0 0.0 3.697561642974541E-5 5 0.0 0.0 0.0 0.0 3.697561642974541E-5 6 0.0 0.0 0.0 0.0 3.697561642974541E-5 7 0.0 0.0 0.0 3.697561642974541E-5 3.697561642974541E-5 8 0.0 0.0 0.0 3.697561642974541E-5 1.1092684928923622E-4 9 0.0 0.0 0.0 1.1092684928923622E-4 1.1092684928923622E-4 10 3.697561642974541E-5 0.0 0.0 1.1092684928923622E-4 1.1092684928923622E-4 11 7.395123285949081E-5 0.0 0.0 1.4790246571898163E-4 1.1092684928923622E-4 12 7.395123285949081E-5 0.0 0.0 2.2185369857847243E-4 1.8487808214872703E-4 13 7.395123285949081E-5 0.0 0.0 2.2185369857847243E-4 2.2185369857847243E-4 14 7.395123285949081E-5 0.0 0.0 2.5882931500821783E-4 2.2185369857847243E-4 15 7.395123285949081E-5 0.0 0.0 2.9580493143796326E-4 2.5882931500821783E-4 16 7.395123285949081E-5 0.0 0.0 2.9580493143796326E-4 4.4370739715694486E-4 17 7.395123285949081E-5 0.0 0.0 2.9580493143796326E-4 4.4370739715694486E-4 18 7.395123285949081E-5 0.0 0.0 3.6975616429745406E-4 4.8068301358669024E-4 19 7.395123285949081E-5 0.0 0.0 4.4370739715694486E-4 4.8068301358669024E-4 20 7.395123285949081E-5 0.0 0.0 5.916098628759265E-4 5.176586300164357E-4 21 7.395123285949081E-5 0.0 0.0 8.504391778841443E-4 5.176586300164357E-4 22 7.395123285949081E-5 0.0 0.0 0.001922732054346761 5.546342464461811E-4 23 7.395123285949081E-5 0.0 0.0 0.002588293150082178 6.285854793056718E-4 24 7.395123285949081E-5 0.0 0.0 0.003956390957982758 6.285854793056718E-4 25 7.395123285949081E-5 0.0 0.0 0.00499170821801563 6.655610957354173E-4 26 7.395123285949081E-5 0.0 0.0 0.0063228304094864645 6.655610957354173E-4 27 7.395123285949081E-5 0.0 0.0 0.011536392326080567 6.655610957354173E-4 28 7.395123285949081E-5 0.0 0.0 0.04581278875645456 6.655610957354173E-4 29 7.395123285949081E-5 0.0 0.0 0.12298090024533322 6.655610957354173E-4 30 1.1092684928923622E-4 0.0 0.0 0.21804521008620864 6.655610957354173E-4 31 1.1092684928923622E-4 0.0 0.0 0.5655050776765262 6.655610957354173E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATACG 120 0.0 23.125002 1 CTTATAC 1965 0.0 17.040712 37 ATACCGT 110 7.817216E-7 16.818182 6 CCCCTAT 245 0.0 15.857143 1 CCCTATA 200 6.184564E-11 14.799999 2 GTTACGG 390 0.0 14.705129 25 CGGTGAT 315 0.0 14.682539 14 CTTACAC 570 0.0 14.605263 3 GTCTTAG 465 0.0 14.32258 1 CTATAGT 210 1.364242E-10 14.095238 4 AACGCAG 7650 0.0 14.026143 5 TCTAACG 120 3.3036056E-5 13.875001 2 CGTATAC 80 0.0063015274 13.875 3 GTCTAAC 295 0.0 13.79661 1 CCTATAC 310 0.0 13.725806 3 ACTAGAC 270 0.0 13.703704 3 CGGGCGT 125 4.849121E-5 13.32 6 CTGTGCG 540 0.0 13.018518 9 ATAATAC 345 0.0 12.869565 3 GAGTACT 5155 0.0 12.775945 11 >>END_MODULE