FastQCFastQC Report
Thu 9 Feb 2017
SRR2727311.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727311.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3964548
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT401671.0131545891234006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT167430.4223180044736499No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT162880.41084128632066No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90930.22935779816513763No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40670.10258420379826401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGT23700.022.8713111
GGTATCA60450.020.3821351
TCGGTGA27550.020.1451913
CGGTGAT27850.019.79533214
GTCGGTG27750.019.73333412
GGCAGTC30400.018.6825668
CGCCTTA21350.018.62997625
GCAGTCG29950.018.5308849
CAGTCGG30050.018.28452510
GTGATTC30200.017.88741916
CTCGCCT22950.017.49237423
GGGCAGT32500.017.418467
TCGCCTT23100.017.37878824
GGTGATT31250.017.345615
TATACCG2350.017.3191495
AACGCAG138700.017.2462165
CTTATAC39750.017.08050337
TCCTCGC24200.016.97107321
TTCCTCG24250.016.93608320
GCCTTAT24050.016.61538526
Produced by FastQC (version 0.11.5)