##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727311.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3964548
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.281127886457675	33.0	31.0	34.0	30.0	34.0
2	32.42782985601385	34.0	31.0	34.0	31.0	34.0
3	32.516214963219014	34.0	31.0	34.0	31.0	34.0
4	36.0614269268527	37.0	35.0	37.0	35.0	37.0
5	35.862662527985535	37.0	35.0	37.0	35.0	37.0
6	35.876472929574824	37.0	35.0	37.0	35.0	37.0
7	35.84462037034234	37.0	35.0	37.0	35.0	37.0
8	35.79709237976183	37.0	35.0	37.0	35.0	37.0
9	37.63182763835877	39.0	37.0	39.0	35.0	39.0
10	37.44858077137671	39.0	37.0	39.0	35.0	39.0
11	37.55955256437808	39.0	37.0	39.0	35.0	39.0
12	37.480021177697935	39.0	37.0	39.0	35.0	39.0
13	37.56330860415866	39.0	37.0	39.0	35.0	39.0
14	38.76573546341222	40.0	38.0	41.0	35.0	41.0
15	38.683716781837425	40.0	38.0	41.0	35.0	41.0
16	38.65692406801482	40.0	38.0	41.0	35.0	41.0
17	38.588225442093275	40.0	38.0	41.0	34.0	41.0
18	38.56526998790278	40.0	38.0	41.0	34.0	41.0
19	38.621195657108956	40.0	38.0	41.0	34.0	41.0
20	38.59126261051701	40.0	38.0	41.0	34.0	41.0
21	38.551381141053156	40.0	38.0	41.0	34.0	41.0
22	38.51165832775893	40.0	38.0	41.0	34.0	41.0
23	38.444393408782034	40.0	38.0	41.0	34.0	41.0
24	38.40576756795478	40.0	38.0	41.0	34.0	41.0
25	38.292537005479566	40.0	38.0	41.0	34.0	41.0
26	38.18021625668298	40.0	38.0	41.0	34.0	41.0
27	38.09419131764832	40.0	38.0	41.0	33.0	41.0
28	37.9969973878485	40.0	37.0	41.0	33.0	41.0
29	37.90627758826479	40.0	37.0	41.0	33.0	41.0
30	37.83319788283557	40.0	37.0	41.0	33.0	41.0
31	37.71638582758993	40.0	37.0	41.0	33.0	41.0
32	37.62274488794183	40.0	37.0	41.0	33.0	41.0
33	37.52875636768681	40.0	37.0	41.0	33.0	41.0
34	37.43130187854958	40.0	36.0	41.0	32.0	41.0
35	37.36198300537665	39.0	36.0	41.0	32.0	41.0
36	37.266069423298696	39.0	36.0	41.0	32.0	41.0
37	37.18089023010946	39.0	36.0	41.0	31.0	41.0
38	37.090345229771465	39.0	36.0	41.0	31.0	41.0
39	36.961614035193925	39.0	36.0	41.0	31.0	41.0
40	36.85198867563213	39.0	36.0	41.0	31.0	41.0
41	36.73258717008849	39.0	35.0	40.0	30.0	41.0
42	36.62114092199161	39.0	35.0	40.0	30.0	41.0
43	34.95568473379563	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	12.0
11	11.0
12	12.0
13	12.0
14	7.0
15	13.0
16	27.0
17	36.0
18	88.0
19	214.0
20	397.0
21	910.0
22	1760.0
23	3214.0
24	5351.0
25	8925.0
26	13771.0
27	20375.0
28	30168.0
29	42458.0
30	57285.0
31	74836.0
32	96553.0
33	126418.0
34	170718.0
35	236117.0
36	347633.0
37	579484.0
38	1083410.0
39	1064332.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.174606537744026	17.552543190295587	12.911509710564737	26.36134056139565
2	16.7852173816536	20.65824401672019	36.84488118191532	25.7116574197109
3	17.924388858452463	21.920582119323566	31.539434003573675	28.615595018650296
4	13.082777658386277	15.554358277412708	35.64878013836634	35.71408392583467
5	13.483428627929337	38.2001933133361	34.95311445340049	13.363263605334074
6	33.975852985006114	36.77440656538904	15.453665840342959	13.796074609261888
7	29.717511302675614	31.658337848349927	20.71807429245402	17.90607655652044
8	28.268291870851353	36.110800020582424	18.81213192525352	16.808776183312702
9	27.653038883625573	13.232832595292074	18.116390569618527	40.997737951463826
10	14.68681927927219	28.265012808521927	34.391461523482626	22.656706388723254
11	37.410241974621066	21.254200983315123	21.577062504981654	19.758494537082157
12	21.367303410123927	25.18284556020005	31.52263511502446	21.92721591465156
13	31.830110267299073	19.20261780157536	24.880793472547186	24.08647845857838
14	21.271302554540895	19.38738035206031	27.830410932091127	31.510906161307673
15	25.306642774914064	28.580685616620105	22.388176407499667	23.724495200966164
16	23.77751511647734	26.96897603459461	24.922538458356414	24.330970390571636
17	23.32520630346763	26.832869724367065	26.53170046118751	23.310223510977796
18	23.76086756926641	26.188433082409396	27.126093567286862	22.924605781037332
19	24.711265950115877	26.274041832763785	27.134316446666805	21.88037577045353
20	23.802410766624593	25.445523676343434	27.291610544253718	23.460455012778255
21	23.408191803958484	25.818327839642752	27.766191757547144	23.007288598851623
22	24.010732118768647	24.996922726121614	26.834383137749878	24.15796201735986
23	23.058341076965142	25.58097417410509	27.33739129908378	24.023293449845983
24	23.09362883234104	25.104021946511935	28.062316309450665	23.740032911696364
25	23.43429818481199	25.07776422432015	27.228602100415987	24.259335490451875
26	23.435584586187378	25.413994230868187	27.685299812235847	23.46512137070859
27	23.709688973370987	25.884413557358872	26.915754330632396	23.490143138637745
28	23.21089314595258	26.259765299852596	26.79109951500146	23.738242039193374
29	23.20605022312758	26.42033845976893	27.373385314038323	23.000226003065166
30	23.838202993128093	26.0618108293808	27.080438930238703	23.019547247252397
31	23.482147271265223	25.721318041804516	27.198939198112875	23.597595488817387
32	22.783126853300807	26.269350251277068	27.42042724668739	23.527095648734736
33	23.182693209919517	25.256170438597287	27.58019325279956	23.980943098683632
34	23.57504562941349	25.624056008402473	27.761928976518885	23.03896938566515
35	23.297157708772854	25.812248962555127	27.25410311591637	23.636490212755653
36	22.899079541980573	25.323138980786712	28.11773246281796	23.660049014414756
37	22.74408078802426	25.23470519211774	28.000342031424513	24.020871988433488
38	23.001310615989514	24.770541307609342	28.20094497531623	24.027203101084915
39	22.47494544144755	24.90054351719288	28.868536842030917	23.75597419932865
40	23.008600223783393	24.534474043447073	28.60747303349587	23.849452699273662
41	21.97579648423982	24.81783547582221	29.12004092269787	24.0863271172401
42	21.721770047934847	24.92594363846774	28.958509267639087	24.393777045958327
43	21.297762065183722	24.51399251566635	28.982572540425792	25.205672878724133
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1103.0
1	1353.0
2	1603.0
3	4188.0
4	6773.0
5	6773.0
6	7956.5
7	9140.0
8	8071.0
9	7002.0
10	9602.5
11	12203.0
12	12203.0
13	18677.5
14	25152.0
15	43988.0
16	62824.0
17	52022.0
18	41220.0
19	41220.0
20	46360.5
21	51501.0
22	41782.5
23	32064.0
24	34928.0
25	37792.0
26	37792.0
27	45122.5
28	52453.0
29	60119.0
30	67785.0
31	78383.5
32	88982.0
33	88982.0
34	101566.0
35	114150.0
36	129181.0
37	144212.0
38	162446.0
39	180680.0
40	180680.0
41	199904.0
42	219128.0
43	239014.5
44	258901.0
45	285087.5
46	311274.0
47	311274.0
48	335768.0
49	360262.0
50	369000.0
51	377738.0
52	360280.0
53	342822.0
54	342822.0
55	318789.5
56	294757.0
57	270988.5
58	247220.0
59	226376.5
60	205533.0
61	205533.0
62	176869.0
63	148205.0
64	122960.5
65	97716.0
66	80459.5
67	63203.0
68	63203.0
69	51271.5
70	39340.0
71	31967.0
72	24594.0
73	19936.0
74	15278.0
75	15278.0
76	12129.5
77	8981.0
78	7311.0
79	5641.0
80	4583.0
81	3525.0
82	3525.0
83	2736.0
84	1947.0
85	1504.0
86	1061.0
87	778.0
88	495.0
89	495.0
90	337.5
91	180.0
92	124.0
93	68.0
94	48.5
95	29.0
96	29.0
97	18.5
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3964548.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.517959232573624
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.31639062421787	43.78685709758568
2	10.268564182800162	11.196423269899297
3	3.4045096321804227	5.568207520023495
4	1.6754000756473135	3.65357572089564
5	0.9791527200242817	2.6690704036373676
6	0.6384164974662199	2.0883098749359488
7	0.4482854647906135	1.7107726085806987
8	0.31901257073534783	1.3913531460822561
9	0.25669491252166243	1.2595034498458537
>10	1.5570829329235298	15.694045572398746
>50	0.09654279668639466	3.55981997705863
>100	0.03699363212340086	3.420249896443569
>500	0.0015692901446686162	0.5930939597074381
>1k	0.0012000453728005706	1.3380219576975236
>5k	4.615559126156041E-5	0.2288079448855377
>10k+	1.3846677378468123E-4	1.841887600322401
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	40167	1.0131545891234006	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16743	0.4223180044736499	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16288	0.41084128632066	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9093	0.22935779816513763	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4067	0.10258420379826401	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.522355638019769E-5	0.0
2	0.0	0.0	0.0	2.522355638019769E-5	0.0
3	0.0	0.0	0.0	5.044711276039538E-5	0.0
4	0.0	0.0	0.0	5.044711276039538E-5	0.0
5	0.0	0.0	0.0	5.044711276039538E-5	0.0
6	0.0	0.0	0.0	5.044711276039538E-5	0.0
7	2.522355638019769E-5	0.0	0.0	5.044711276039538E-5	0.0
8	1.0089422552079076E-4	0.0	0.0	5.044711276039538E-5	2.522355638019769E-5
9	1.7656489466138383E-4	0.0	0.0	7.567066914059307E-5	2.522355638019769E-5
10	3.2790623294257E-4	0.0	0.0	1.2611778190098845E-4	2.522355638019769E-5
11	3.783533457029654E-4	0.0	0.0	2.0178845104158153E-4	2.522355638019769E-5
12	3.783533457029654E-4	0.0	0.0	3.2790623294257E-4	2.522355638019769E-5
13	3.783533457029654E-4	0.0	0.0	4.0357690208316305E-4	2.522355638019769E-5
14	5.296946839841516E-4	0.0	0.0	4.2880045846336077E-4	5.044711276039538E-5
15	5.549182403643492E-4	0.0	2.522355638019769E-5	5.296946839841516E-4	1.0089422552079076E-4
16	5.801417967445469E-4	0.0	2.522355638019769E-5	7.314831350257331E-4	1.2611778190098845E-4
17	5.801417967445469E-4	0.0	2.522355638019769E-5	8.576009169267215E-4	1.2611778190098845E-4
18	6.053653531247446E-4	0.0	2.522355638019769E-5	9.8371869882771E-4	1.2611778190098845E-4
19	6.5581246588514E-4	0.0	2.522355638019769E-5	0.0012107307062494892	1.5134133828118614E-4
20	7.314831350257331E-4	0.0	2.522355638019769E-5	0.0019422138412752224	1.5134133828118614E-4
21	9.332715860673146E-4	0.0	2.522355638019769E-5	0.0028250383145821414	1.7656489466138383E-4
22	9.8371869882771E-4	0.0	2.522355638019769E-5	0.004893369937758353	1.7656489466138383E-4
23	9.8371869882771E-4	0.0	2.522355638019769E-5	0.0058770886365860624	2.2701200742177922E-4
24	9.8371869882771E-4	0.0	2.522355638019769E-5	0.007667961139580098	2.2701200742177922E-4
25	9.8371869882771E-4	0.0	2.522355638019769E-5	0.008323773605465238	2.522355638019769E-4
26	9.8371869882771E-4	0.0	2.522355638019769E-5	0.009357939417053344	2.774591201821746E-4
27	9.8371869882771E-4	0.0	2.522355638019769E-5	0.019296020630851233	2.774591201821746E-4
28	9.8371869882771E-4	0.0	2.522355638019769E-5	0.08929138958589983	2.774591201821746E-4
29	9.8371869882771E-4	0.0	2.522355638019769E-5	0.22597784161019113	2.774591201821746E-4
30	9.8371869882771E-4	0.0	2.522355638019769E-5	0.3713916441420308	2.774591201821746E-4
31	9.8371869882771E-4	0.0	2.522355638019769E-5	0.8390616029872763	2.774591201821746E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTCGGT	2370	0.0	22.87131	11
GGTATCA	6045	0.0	20.382135	1
TCGGTGA	2755	0.0	20.14519	13
CGGTGAT	2785	0.0	19.795332	14
GTCGGTG	2775	0.0	19.733334	12
GGCAGTC	3040	0.0	18.682566	8
CGCCTTA	2135	0.0	18.629976	25
GCAGTCG	2995	0.0	18.530884	9
CAGTCGG	3005	0.0	18.284525	10
GTGATTC	3020	0.0	17.887419	16
CTCGCCT	2295	0.0	17.492374	23
GGGCAGT	3250	0.0	17.41846	7
TCGCCTT	2310	0.0	17.378788	24
GGTGATT	3125	0.0	17.3456	15
TATACCG	235	0.0	17.319149	5
AACGCAG	13870	0.0	17.246216	5
CTTATAC	3975	0.0	17.080503	37
TCCTCGC	2420	0.0	16.971073	21
TTCCTCG	2425	0.0	16.936083	20
GCCTTAT	2405	0.0	16.615385	26
>>END_MODULE