FastQCFastQC Report
Thu 9 Feb 2017
SRR2727310.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727310.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2225877
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT158020.7099224260819443No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT57080.2564382488340551No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54520.2449371640930743No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39870.17912040961832124No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA400.001931752423.12514
CGTTACG4350.017.8620724
AGTGTAC10300.017.6019427
CGACCGA1401.87174E-917.17857218
CTTATAC28150.016.82415637
TCTATAC2800.016.5178573
TACGGAT9100.016.46703327
ACGGATG9250.016.428
GTTACGG7950.016.28930925
TACTTAC8750.016.2831
GTACTTA9450.016.0529130
TCGTTAC6100.015.77049323
GTCTTAG4050.015.5308641
ACGACCG1557.219569E-915.516129517
CGTACTA1201.9373856E-615.41666629
CTCGTTA6650.015.30075222
TAACCCG855.3669536E-415.2352945
GTGTACT10350.015.19323528
CGATTTG1954.1836756E-1115.17948722
TATACCG1353.976711E-715.0740735
Produced by FastQC (version 0.11.5)