##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727310.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2225877
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25246363568158	33.0	31.0	34.0	30.0	34.0
2	32.401975940269836	34.0	31.0	34.0	31.0	34.0
3	32.489903080898	34.0	31.0	34.0	31.0	34.0
4	36.04501057336052	37.0	35.0	37.0	35.0	37.0
5	35.82634799676712	37.0	35.0	37.0	35.0	37.0
6	35.84196341486973	37.0	35.0	37.0	35.0	37.0
7	35.807262036491686	37.0	35.0	37.0	35.0	37.0
8	35.75839949826518	37.0	35.0	37.0	35.0	37.0
9	37.58580550497624	39.0	37.0	39.0	35.0	39.0
10	37.395724921008664	39.0	37.0	39.0	34.0	39.0
11	37.509239279618775	39.0	37.0	39.0	35.0	39.0
12	37.4307843605015	39.0	37.0	39.0	35.0	39.0
13	37.51097163050788	39.0	37.0	39.0	35.0	39.0
14	38.71764252921433	40.0	38.0	41.0	35.0	41.0
15	38.64088357083523	40.0	38.0	41.0	35.0	41.0
16	38.59709678477292	40.0	38.0	41.0	34.0	41.0
17	38.53619629476382	40.0	38.0	41.0	34.0	41.0
18	38.50116021684936	40.0	38.0	41.0	34.0	41.0
19	38.56323148134421	40.0	38.0	41.0	34.0	41.0
20	38.52090659097515	40.0	38.0	41.0	34.0	41.0
21	38.47821600205222	40.0	38.0	41.0	34.0	41.0
22	38.44780956000714	40.0	38.0	41.0	34.0	41.0
23	38.37281260375124	40.0	38.0	41.0	34.0	41.0
24	38.33246221601643	40.0	38.0	41.0	34.0	41.0
25	38.209759568924966	40.0	38.0	41.0	34.0	41.0
26	38.10210806796602	40.0	38.0	41.0	33.0	41.0
27	38.00174987207289	40.0	37.0	41.0	33.0	41.0
28	37.896598958522866	40.0	37.0	41.0	33.0	41.0
29	37.80388359284902	40.0	37.0	41.0	33.0	41.0
30	37.74425316403377	40.0	37.0	41.0	33.0	41.0
31	37.63777558238842	40.0	37.0	41.0	33.0	41.0
32	37.5389278023898	40.0	37.0	41.0	33.0	41.0
33	37.443916712378986	39.0	37.0	41.0	32.0	41.0
34	37.34275433907624	39.0	36.0	41.0	32.0	41.0
35	37.2730815763854	39.0	36.0	41.0	32.0	41.0
36	37.1628099845589	39.0	36.0	41.0	31.0	41.0
37	37.083741824009145	39.0	36.0	41.0	31.0	41.0
38	36.98808110241492	39.0	36.0	41.0	31.0	41.0
39	36.86419734783189	39.0	36.0	40.0	31.0	41.0
40	36.75133396858856	39.0	36.0	40.0	30.0	41.0
41	36.622216321926146	39.0	35.0	40.0	30.0	41.0
42	36.510838649215565	39.0	35.0	40.0	30.0	41.0
43	34.780035015411904	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	8.0
11	4.0
12	5.0
13	1.0
14	0.0
15	1.0
16	17.0
17	18.0
18	49.0
19	104.0
20	268.0
21	578.0
22	1076.0
23	1906.0
24	3378.0
25	5495.0
26	8357.0
27	12381.0
28	17965.0
29	25116.0
30	33167.0
31	43126.0
32	55253.0
33	72414.0
34	98127.0
35	134803.0
36	199233.0
37	331721.0
38	628175.0
39	553128.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.24907575755533	16.922004225750122	12.509406404756419	26.319513611938127
2	16.70644873908127	20.404811227215163	37.40867082952023	25.48006920418334
3	18.70849108014504	22.29354991313536	30.70313409051803	28.294824916201566
4	13.678967885467166	14.775120098729625	34.85340834197038	36.69250367383283
5	14.364001245351831	37.40557092777364	34.57131728303046	13.659110543844067
6	35.58714160755513	35.63040545367062	14.654897822296558	14.127555116477685
7	29.74751075643443	31.075661413456356	20.87869185943338	18.298135970675826
8	27.703776983184607	36.895704479627575	18.862497793004735	16.53802074418308
9	27.490422876016957	13.459413974806333	17.864778691724656	41.18538445745205
10	14.880606610338306	27.847855025232747	34.26954858691653	23.001989777512414
11	37.508047389860266	20.957627038690816	21.190568930807945	20.34375664064097
12	21.105748430843214	24.661560364746123	31.358246659631238	22.87444454477943
13	31.96465932304435	18.84376360418837	24.502701631761322	24.68887544100595
14	21.73188365754262	19.09418175397832	27.20689418148442	31.967040406994634
15	25.749625877800074	27.768829993750778	22.17319285836549	24.308351270083655
16	24.256506536524704	26.095691720611697	24.795305400972293	24.85249634189131
17	24.063683662664197	26.321086025867558	25.755915533517797	23.859314777950445
18	23.846915170964074	26.007906097237175	26.550972942350366	23.594205789448385
19	25.413848114698162	25.761531297551482	26.065231816493007	22.759388771257353
20	24.05227243014776	25.420002992079077	26.283168387112138	24.24455619066103
21	23.648206976396267	25.971560872411185	26.86105296923415	23.519179181958393
22	24.509170991928126	24.937541472417387	25.72374843713287	24.829539098521618
23	23.62843948699771	25.195731839629953	26.666837385893288	24.50899128747905
24	23.563656033105154	24.928017136616266	26.818822423700862	24.689504406577722
25	24.234178258726786	25.092042372512047	26.125926994169042	24.54785237459213
26	23.769507479523803	25.310248499804793	26.8715656795052	24.0486783411662
27	24.582580259376417	25.859065887288473	26.074127186722357	23.484226666612756
28	24.082507703705105	26.328633612728826	25.596337982736696	23.99252070082938
29	23.734375259729088	26.24897961567508	26.440050371157074	23.576594753438755
30	24.66807465102519	25.701554937671755	25.883505692363055	23.746864718939996
31	23.979671832720317	25.22336139867567	26.557487228629434	24.23947953997458
32	23.413782522574248	25.90610352683459	26.35329804836476	24.326815902226404
33	23.868075369842987	24.982737141360463	26.410623767620585	24.738563721175968
34	24.111170563333015	25.374852249248274	26.984464999638348	23.529512187780366
35	23.92966906976441	25.370584268582675	26.44234160288282	24.257405058770093
36	23.75072836459517	25.005739310842422	27.0535164341965	24.19001589036591
37	23.095031756022458	24.939338516908165	27.258065023359336	24.707564703710045
38	23.769282848962455	24.60706499056327	27.252269554876573	24.371382605597706
39	22.90090602490614	24.728904607038036	27.993640259547135	24.37654910850869
40	23.91425941325599	24.32555797108286	27.835230787685035	23.92495182797612
41	23.005359235932623	24.552075429145457	27.909224094592826	24.533341240329094
42	22.611941270788996	24.696872288989912	28.05038193934346	24.640804500877632
43	22.17144073998698	24.175414903878337	27.754902898947247	25.89824145718744
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	108.0
1	203.0
2	298.0
3	1526.0
4	2754.0
5	2754.0
6	3681.0
7	4608.0
8	4264.5
9	3921.0
10	4906.0
11	5891.0
12	5891.0
13	9324.5
14	12758.0
15	20464.0
16	28170.0
17	22243.5
18	16317.0
19	16317.0
20	17304.5
21	18292.0
22	15427.5
23	12563.0
24	15321.0
25	18079.0
26	18079.0
27	21704.5
28	25330.0
29	29248.0
30	33166.0
31	38359.5
32	43553.0
33	43553.0
34	50573.0
35	57593.0
36	66535.5
37	75478.0
38	86281.5
39	97085.0
40	97085.0
41	108696.5
42	120308.0
43	133689.0
44	147070.0
45	162484.5
46	177899.0
47	177899.0
48	191407.5
49	204916.0
50	210520.5
51	216125.0
52	213222.0
53	210319.0
54	210319.0
55	194222.0
56	178125.0
57	162606.5
58	147088.0
59	134399.5
60	121711.0
61	121711.0
62	105532.5
63	89354.0
64	74433.0
65	59512.0
66	49143.5
67	38775.0
68	38775.0
69	31306.0
70	23837.0
71	19111.5
72	14386.0
73	11642.5
74	8899.0
75	8899.0
76	7080.5
77	5262.0
78	4133.5
79	3005.0
80	2321.0
81	1637.0
82	1637.0
83	1260.0
84	883.0
85	675.5
86	468.0
87	346.5
88	225.0
89	225.0
90	147.0
91	69.0
92	45.5
93	22.0
94	17.0
95	12.0
96	12.0
97	8.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2225877.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.52403176568249
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.10710469866555	50.299770447359336
2	9.03053094351642	10.931282833727327
3	2.8628923154829753	5.1982135633205955
4	1.3855679033292854	3.3544062317844694
5	0.8160947844784432	2.4696673329790544
6	0.5387462033689864	1.956425539580722
7	0.3955734719246066	1.675919096629834
8	0.2910674380109628	1.4093259891305063
9	0.2283839031071701	1.2440443145786022
>10	1.251599294223208	13.542719964153596
>50	0.06293603779324183	2.5922898192188666
>100	0.02735015835495437	2.8719998456388804
>500	0.001187778065780324	0.4931593452533317
>1k	7.423612911126302E-4	0.7493541873300339
>5k	1.4847225822252604E-4	0.501426593752439
>10k+	7.423612911126302E-5	0.7099948955623693
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15802	0.7099224260819443	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5708	0.2564382488340551	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5452	0.2449371640930743	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3987	0.17912040961832124	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.492611226945604E-5	0.0
2	0.0	0.0	0.0	8.985222453891207E-5	0.0
3	0.0	0.0	0.0	1.3477833680836812E-4	0.0
4	0.0	0.0	0.0	1.3477833680836812E-4	0.0
5	4.492611226945604E-5	0.0	0.0	1.3477833680836812E-4	0.0
6	4.492611226945604E-5	0.0	0.0	1.3477833680836812E-4	0.0
7	4.492611226945604E-5	0.0	0.0	1.3477833680836812E-4	0.0
8	4.492611226945604E-5	0.0	0.0	1.3477833680836812E-4	0.0
9	4.492611226945604E-5	0.0	0.0	1.7970444907782415E-4	8.985222453891207E-5
10	4.492611226945604E-5	0.0	0.0	1.7970444907782415E-4	8.985222453891207E-5
11	8.985222453891207E-5	0.0	0.0	2.246305613472802E-4	8.985222453891207E-5
12	8.985222453891207E-5	0.0	0.0	3.594088981556483E-4	8.985222453891207E-5
13	8.985222453891207E-5	0.0	0.0	4.492611226945604E-4	8.985222453891207E-5
14	8.985222453891207E-5	0.0	0.0	4.941872349640164E-4	8.985222453891207E-5
15	1.3477833680836812E-4	0.0	0.0	7.637439085807526E-4	1.3477833680836812E-4
16	1.3477833680836812E-4	0.0	0.0	0.0013028572558142252	1.3477833680836812E-4
17	1.3477833680836812E-4	0.0	0.0	0.0017521183785087855	1.3477833680836812E-4
18	1.3477833680836812E-4	0.0	0.0	0.00215645338893389	1.3477833680836812E-4
19	1.3477833680836812E-4	0.0	0.0	0.0026057145116284504	1.3477833680836812E-4
20	1.3477833680836812E-4	0.0	0.0	0.004133202328789955	1.3477833680836812E-4
21	1.7970444907782415E-4	0.0	0.0	0.005660690145951461	1.3477833680836812E-4
22	1.7970444907782415E-4	0.0	0.0	0.008850444117082839	2.246305613472802E-4
23	1.7970444907782415E-4	0.0	0.0	0.010872119169208362	3.1448278588619227E-4
24	1.7970444907782415E-4	0.0	0.0	0.015005321497998316	3.1448278588619227E-4
25	1.7970444907782415E-4	0.0	0.0	0.017970444907782417	3.1448278588619227E-4
26	1.7970444907782415E-4	0.0	0.0	0.022507982246997477	3.1448278588619227E-4
27	1.7970444907782415E-4	0.0	0.0	0.036614781499606674	3.1448278588619227E-4
28	1.7970444907782415E-4	0.0	0.0	0.14169695809786434	3.1448278588619227E-4
29	1.7970444907782415E-4	0.0	0.0	0.3282751023529153	3.1448278588619227E-4
30	1.7970444907782415E-4	0.0	0.0	0.551872363117998	3.1448278588619227E-4
31	1.7970444907782415E-4	0.0	0.0	1.0936812770876378	3.1448278588619227E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATA	40	0.0019317524	23.125	14
CGTTACG	435	0.0	17.86207	24
AGTGTAC	1030	0.0	17.60194	27
CGACCGA	140	1.87174E-9	17.178572	18
CTTATAC	2815	0.0	16.824156	37
TCTATAC	280	0.0	16.517857	3
TACGGAT	910	0.0	16.467033	27
ACGGATG	925	0.0	16.4	28
GTTACGG	795	0.0	16.289309	25
TACTTAC	875	0.0	16.28	31
GTACTTA	945	0.0	16.05291	30
TCGTTAC	610	0.0	15.770493	23
GTCTTAG	405	0.0	15.530864	1
ACGACCG	155	7.219569E-9	15.5161295	17
CGTACTA	120	1.9373856E-6	15.416666	29
CTCGTTA	665	0.0	15.300752	22
TAACCCG	85	5.3669536E-4	15.235294	5
GTGTACT	1035	0.0	15.193235	28
CGATTTG	195	4.1836756E-11	15.179487	22
TATACCG	135	3.976711E-7	15.074073	5
>>END_MODULE