FastQCFastQC Report
Thu 9 Feb 2017
SRR2727309.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727309.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1861319
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT127220.6834938019759106No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT43410.2332217099809329No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40230.21613705119863927No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32030.17208227069083804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG705.1047246E-621.1428595
GCGTTAT555.14474E-420.1818181
GACCGTA1201.04219E-716.95833426
TATACAC10800.016.27314837
CTTATAC26300.015.89733937
TGATCGC1201.936969E-615.4166679
TCCTAGA2550.015.2352942
GTCCTAG3200.015.031251
ACCCGAC5800.014.99137911
AACGCAG56400.014.82624155
CGGTGAT3250.014.814
ATACCGT1252.9609455E-614.7999996
ATACCGA1001.09394205E-414.7999996
ATAAGAC2301.8189894E-1214.478263
TATACCG1152.2111888E-514.478265
GTATAAG2750.014.1272741
CTAGTAC1051.6565005E-414.0952383
GTATCGC1051.6565005E-414.0952386
GTATTAA3950.014.0506321
GTATTAC1854.9039954E-914.01
Produced by FastQC (version 0.11.5)