##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727309.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1861319
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.179026271155024	33.0	31.0	34.0	30.0	34.0
2	32.334755084969316	34.0	31.0	34.0	30.0	34.0
3	32.42663186697175	34.0	31.0	34.0	30.0	34.0
4	36.003018826971626	37.0	35.0	37.0	35.0	37.0
5	35.776404796813445	37.0	35.0	37.0	35.0	37.0
6	35.78169835476885	37.0	35.0	37.0	35.0	37.0
7	35.75188723695401	37.0	35.0	37.0	33.0	37.0
8	35.6994744049784	37.0	35.0	37.0	33.0	37.0
9	37.52626497661067	39.0	37.0	39.0	35.0	39.0
10	37.33474057912695	39.0	37.0	39.0	34.0	39.0
11	37.43979618754228	39.0	37.0	39.0	35.0	39.0
12	37.35946981683419	39.0	37.0	39.0	34.0	39.0
13	37.44511445915504	39.0	37.0	39.0	35.0	39.0
14	38.64032656411931	40.0	38.0	41.0	35.0	41.0
15	38.55216327776163	40.0	38.0	41.0	34.0	41.0
16	38.521763867450986	40.0	38.0	41.0	34.0	41.0
17	38.44344628728337	40.0	38.0	41.0	34.0	41.0
18	38.41301034374011	40.0	38.0	41.0	34.0	41.0
19	38.46143406906608	40.0	38.0	41.0	34.0	41.0
20	38.41622258194324	40.0	38.0	41.0	34.0	41.0
21	38.37389560843681	40.0	38.0	41.0	34.0	41.0
22	38.33619223787003	40.0	38.0	41.0	34.0	41.0
23	38.262604099565955	40.0	38.0	41.0	34.0	41.0
24	38.21685267275518	40.0	38.0	41.0	34.0	41.0
25	38.09758080157136	40.0	38.0	41.0	33.0	41.0
26	37.98031288564722	40.0	37.0	41.0	33.0	41.0
27	37.876685296824455	40.0	37.0	41.0	33.0	41.0
28	37.77468988389416	40.0	37.0	41.0	33.0	41.0
29	37.689590553795455	40.0	37.0	41.0	33.0	41.0
30	37.619591805595924	40.0	37.0	41.0	33.0	41.0
31	37.526449791787435	39.0	37.0	41.0	32.0	41.0
32	37.43064407551849	39.0	37.0	41.0	32.0	41.0
33	37.32604835603139	39.0	36.0	41.0	32.0	41.0
34	37.23203008189354	39.0	36.0	41.0	31.0	41.0
35	37.16467085975053	39.0	36.0	41.0	31.0	41.0
36	37.07421618755302	39.0	36.0	41.0	31.0	41.0
37	36.98300882331293	39.0	36.0	40.0	31.0	41.0
38	36.88251288468017	39.0	36.0	40.0	31.0	41.0
39	36.76828797213159	39.0	36.0	40.0	30.0	41.0
40	36.64407498123642	39.0	35.0	40.0	30.0	41.0
41	36.53070859965433	39.0	35.0	40.0	30.0	41.0
42	36.415726697035815	39.0	35.0	40.0	30.0	41.0
43	34.67005548216077	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	2.0
11	2.0
12	2.0
13	2.0
14	6.0
15	5.0
16	4.0
17	22.0
18	54.0
19	129.0
20	285.0
21	511.0
22	995.0
23	1815.0
24	3021.0
25	4948.0
26	7416.0
27	11242.0
28	16057.0
29	21775.0
30	29313.0
31	37741.0
32	48773.0
33	63808.0
34	84474.0
35	116976.0
36	171575.0
37	283063.0
38	525087.0
39	432213.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.865989655722636	17.213062349871247	12.580755904818036	26.340192089588083
2	16.883940904272723	20.249779860410815	36.92279507166692	25.94348416364954
3	18.62996079661788	21.976136277553714	30.697209881809616	28.696693044018783
4	13.652952556762166	14.941823513325767	34.83798317214835	36.56724075776371
5	14.451096238742526	36.99516310745229	34.60852223611321	13.94521841769197
6	35.58804267296471	35.736539518481244	14.676044246042725	13.999373562511316
7	29.93839315023379	30.691085192812196	20.81604496596231	18.554476690991713
8	27.798244148370056	36.306887750031024	18.83406337119	17.06080473040892
9	26.772949720064105	13.555226159513763	18.09732775521015	41.574496365211985
10	15.122286937381501	27.529080184535804	34.23013465182486	23.11849822625783
11	37.43689287005613	20.781338394976896	21.426149950653272	20.3556187843137
12	21.28361661810791	24.677661378839414	31.234785654688963	22.803936348363717
13	31.976517727482502	18.6919598413813	24.511703797146005	24.8198186339902
14	21.870136177624577	18.99593782688513	26.99241774247187	32.14150825301842
15	25.43594085699442	27.67215077050199	22.177767486390028	24.714140886113555
16	24.647091659194366	25.825073509699305	24.42842951691784	25.099405314188488
17	24.046173707999543	26.26476170930399	25.988452274972744	23.700612307723716
18	24.239746115523452	25.471238406742746	26.389028425541245	23.899987052192557
19	25.299908290841067	25.560315023915837	26.137164021857618	23.00261266338548
20	24.454593758512107	25.35239795005585	26.119918187049073	24.073090104382967
21	24.042359208711673	25.709832650931947	26.271746003774744	23.97606213658164
22	24.638226977750723	25.058735230231893	25.74631215820609	24.55672563381129
23	23.940710861491233	25.13266130093767	26.217053605534566	24.70957423203653
24	24.071048541383824	24.983466025974053	26.384408046122132	24.56107738651999
25	24.499669320519484	24.951069644698194	26.04679799647454	24.50246303830778
26	24.147875780562064	25.257626446621995	26.457581962038745	24.136915810777197
27	24.66471357139749	25.53957704187192	26.000970279678015	23.794739107052578
28	24.40898094308391	25.796975155790058	25.791065368160965	24.002978532965063
29	24.004160490490882	25.838236218509564	26.16424159426729	23.99336169673226
30	24.259517041409882	25.61935917486471	26.08617867222115	24.03494511150426
31	24.0813638070637	25.035687058478423	26.287380078320805	24.595569056137073
32	23.586714582508424	25.539254689819423	26.393702530302438	24.48032819736971
33	24.013831052065765	24.952412778250263	26.331596034854854	24.702160134829118
34	24.14277187306421	24.96938998634839	26.824311147095152	24.06352699349225
35	23.944417910094938	24.991148749891877	26.580236918013515	24.48419642199967
36	23.77314151953534	24.61996036144261	26.96061233995892	24.646285779063128
37	23.43209304799446	24.34348975108512	27.06204578581103	25.16237141510939
38	23.443267919147658	24.29648007676277	27.50033712652157	24.759914877568004
39	23.15374205066407	24.11934762391616	27.99530870312934	24.73160162229043
40	23.432630301415287	24.122839771151533	28.050269728079925	24.394260199353255
41	22.860938936313442	23.90965761376744	28.37310530865478	24.85629814126434
42	22.342650561241786	24.170494149578875	28.33925834314269	25.147596946036654
43	22.116896673810345	23.627116039754604	28.363757099132393	25.892230187302662
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	69.0
1	126.5
2	184.0
3	1088.5
4	1993.0
5	1993.0
6	2700.5
7	3408.0
8	3192.5
9	2977.0
10	3816.0
11	4655.0
12	4655.0
13	7323.5
14	9992.0
15	16055.5
16	22119.0
17	17107.0
18	12095.0
19	12095.0
20	13016.5
21	13938.0
22	11885.0
23	9832.0
24	12040.0
25	14248.0
26	14248.0
27	17405.5
28	20563.0
29	23695.0
30	26827.0
31	31668.0
32	36509.0
33	36509.0
34	42497.5
35	48486.0
36	56114.5
37	63743.0
38	73097.5
39	82452.0
40	82452.0
41	92822.0
42	103192.0
43	114341.5
44	125491.0
45	136566.0
46	147641.0
47	147641.0
48	158555.5
49	169470.0
50	171916.0
51	174362.0
52	172193.0
53	170024.0
54	170024.0
55	159346.0
56	148668.0
57	136438.5
58	124209.0
59	114073.5
60	103938.0
61	103938.0
62	90876.5
63	77815.0
64	65226.0
65	52637.0
66	43687.0
67	34737.0
68	34737.0
69	28392.0
70	22047.0
71	17885.0
72	13723.0
73	11085.0
74	8447.0
75	8447.0
76	6732.0
77	5017.0
78	3902.0
79	2787.0
80	2212.5
81	1638.0
82	1638.0
83	1204.5
84	771.0
85	568.0
86	365.0
87	263.0
88	161.0
89	161.0
90	109.0
91	57.0
92	40.0
93	23.0
94	14.5
95	6.0
96	6.0
97	4.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1861319.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.14020451377292
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.19581186063763	52.53000575570036
2	9.046544562892166	11.424013476879438
3	2.895922608192974	5.485474372120893
4	1.4246119367339054	3.5980115615256367
5	0.8262294842931432	2.6084149306789106
6	0.536926621664583	2.0340994020474574
7	0.3707680464792599	1.6387259197310777
8	0.281093016668106	1.4198616447854073
9	0.21149212700164172	1.2018290536742848
>10	1.1448529525297448	12.51124314770546
>50	0.048129874496694695	2.0358669827455023
>100	0.016425879059101832	1.7186213666331174
>500	4.253676253588883E-4	0.16888183922349398
>1k	6.805882005742211E-4	0.9415807123882396
>5k	0.0	0.0
>10k+	8.507352507177763E-5	0.6833698341607156
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12722	0.6834938019759106	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4341	0.2332217099809329	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4023	0.21613705119863927	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3203	0.17208227069083804	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.3725342082684375E-5	5.3725342082684375E-5
2	0.0	0.0	0.0	5.3725342082684375E-5	5.3725342082684375E-5
3	0.0	0.0	0.0	5.3725342082684375E-5	5.3725342082684375E-5
4	0.0	0.0	0.0	5.3725342082684375E-5	5.3725342082684375E-5
5	0.0	0.0	0.0	5.3725342082684375E-5	5.3725342082684375E-5
6	0.0	0.0	0.0	5.3725342082684375E-5	5.3725342082684375E-5
7	0.0	0.0	0.0	5.3725342082684375E-5	5.3725342082684375E-5
8	0.0	0.0	0.0	5.3725342082684375E-5	1.0745068416536875E-4
9	0.0	0.0	0.0	5.3725342082684375E-5	1.0745068416536875E-4
10	0.0	0.0	0.0	5.3725342082684375E-5	1.0745068416536875E-4
11	0.0	0.0	0.0	5.3725342082684375E-5	1.0745068416536875E-4
12	0.0	0.0	0.0	1.6117602624805312E-4	1.0745068416536875E-4
13	0.0	0.0	0.0	1.6117602624805312E-4	1.0745068416536875E-4
14	0.0	0.0	0.0	1.6117602624805312E-4	1.0745068416536875E-4
15	5.3725342082684375E-5	0.0	0.0	3.7607739457879065E-4	2.149013683307375E-4
16	5.3725342082684375E-5	0.0	0.0	8.058801312402656E-4	3.2235205249610624E-4
17	5.3725342082684375E-5	0.0	0.0	0.0011819575258190562	3.2235205249610624E-4
18	5.3725342082684375E-5	0.0	0.0	0.0014505842362324782	3.2235205249610624E-4
19	5.3725342082684375E-5	0.0	0.0	0.0020952883412246907	3.2235205249610624E-4
20	1.0745068416536875E-4	0.0	0.0	0.0034921472353744844	3.2235205249610624E-4
21	1.6117602624805312E-4	0.0	0.0	0.005372534208268437	3.2235205249610624E-4
22	2.149013683307375E-4	0.0	0.0	0.008166251996568025	3.2235205249610624E-4
23	2.149013683307375E-4	0.0	0.0	0.010046638969461978	4.29802736661475E-4
24	2.149013683307375E-4	0.0	0.0	0.014183490309828675	4.835280787441594E-4
25	2.149013683307375E-4	0.0	0.0	0.01595642659855726	4.835280787441594E-4
26	2.149013683307375E-4	0.0	0.0	0.019985827254758587	4.835280787441594E-4
27	2.149013683307375E-4	0.0	0.0	0.036694408642473426	4.835280787441594E-4
28	2.149013683307375E-4	0.0	0.0	0.16622620840382546	4.835280787441594E-4
29	2.149013683307375E-4	0.0	0.0	0.39386048280815916	4.835280787441594E-4
30	2.149013683307375E-4	0.0	0.0	0.6578130884603876	4.835280787441594E-4
31	2.149013683307375E-4	0.0	0.0	1.2687776786246743	4.835280787441594E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACGG	70	5.1047246E-6	21.142859	5
GCGTTAT	55	5.14474E-4	20.181818	1
GACCGTA	120	1.04219E-7	16.958334	26
TATACAC	1080	0.0	16.273148	37
CTTATAC	2630	0.0	15.897339	37
TGATCGC	120	1.936969E-6	15.416667	9
TCCTAGA	255	0.0	15.235294	2
GTCCTAG	320	0.0	15.03125	1
ACCCGAC	580	0.0	14.991379	11
AACGCAG	5640	0.0	14.8262415	5
CGGTGAT	325	0.0	14.8	14
ATACCGT	125	2.9609455E-6	14.799999	6
ATACCGA	100	1.09394205E-4	14.799999	6
ATAAGAC	230	1.8189894E-12	14.47826	3
TATACCG	115	2.2111888E-5	14.47826	5
GTATAAG	275	0.0	14.127274	1
CTAGTAC	105	1.6565005E-4	14.095238	3
GTATCGC	105	1.6565005E-4	14.095238	6
GTATTAA	395	0.0	14.050632	1
GTATTAC	185	4.9039954E-9	14.0	1
>>END_MODULE