FastQCFastQC Report
Thu 9 Feb 2017
SRR2727308.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727308.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2273854
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT210580.9260928801937152No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT73490.32319577246384334No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT68510.3012946301741449No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53510.23532733412083626No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA25580.11249622886957562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG1200.023.1249985
TATACCG1059.840733E-1021.1428595
ATACGAT852.7250138E-517.4117646
TACGTAG650.001580576417.07692312
ACGCGCA1851.8189894E-1217.019
AATCGGG1757.2759576E-1216.91428625
TACCGAC1107.81587E-716.8181827
ACCGATT2000.016.65000220
GTCTTAT2700.016.4444431
AGTCGGT2850.016.22807111
CAATCGG2300.016.08695624
CGACCGA1851.8189894E-1116.018
AACGCAG82650.015.8923185
TCTTAGA8050.015.6273282
CGACGCG1902.7284841E-1115.57894817
ATACCGA957.064534E-515.5789486
CGCGCAA2150.015.48837220
CGCGAAC855.367019E-415.23529412
CTTATAC22900.015.18777437
CGCAATC1954.1836756E-1115.17948722
Produced by FastQC (version 0.11.5)