##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727308.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2273854
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.225090968901256	33.0	31.0	34.0	30.0	34.0
2	32.366924613453634	34.0	31.0	34.0	30.0	34.0
3	32.45549714273652	34.0	31.0	34.0	30.0	34.0
4	36.01778522279795	37.0	35.0	37.0	35.0	37.0
5	35.801726056290335	37.0	35.0	37.0	35.0	37.0
6	35.81658101179759	37.0	35.0	37.0	35.0	37.0
7	35.781884852765394	37.0	35.0	37.0	35.0	37.0
8	35.735154499805176	37.0	35.0	37.0	33.0	37.0
9	37.561152123223394	39.0	37.0	39.0	35.0	39.0
10	37.36430879027414	39.0	37.0	39.0	34.0	39.0
11	37.480235758320454	39.0	37.0	39.0	35.0	39.0
12	37.39492992953813	39.0	37.0	39.0	34.0	39.0
13	37.48529193167195	39.0	37.0	39.0	35.0	39.0
14	38.67904139843631	40.0	38.0	41.0	35.0	41.0
15	38.59786204391311	40.0	38.0	41.0	34.0	41.0
16	38.567141074141084	40.0	38.0	41.0	34.0	41.0
17	38.49944983275091	40.0	38.0	41.0	34.0	41.0
18	38.470568031192855	40.0	38.0	41.0	34.0	41.0
19	38.520926585436	40.0	38.0	41.0	34.0	41.0
20	38.48188626006771	40.0	38.0	41.0	34.0	41.0
21	38.436047784950134	40.0	38.0	41.0	34.0	41.0
22	38.39112273699191	40.0	38.0	41.0	34.0	41.0
23	38.32092034053198	40.0	38.0	41.0	34.0	41.0
24	38.27503832699901	40.0	38.0	41.0	34.0	41.0
25	38.15561289335199	40.0	38.0	41.0	33.0	41.0
26	38.031971709705196	40.0	37.0	41.0	33.0	41.0
27	37.929524059152435	40.0	37.0	41.0	33.0	41.0
28	37.830312324362076	40.0	37.0	41.0	33.0	41.0
29	37.732919088032915	40.0	37.0	41.0	33.0	41.0
30	37.65172258201274	40.0	37.0	41.0	33.0	41.0
31	37.54233033431346	39.0	37.0	41.0	32.0	41.0
32	37.437665742831335	39.0	36.0	41.0	32.0	41.0
33	37.32923705743641	39.0	36.0	41.0	32.0	41.0
34	37.21409026261141	39.0	36.0	41.0	31.0	41.0
35	37.12543945213721	39.0	36.0	41.0	31.0	41.0
36	37.02767899786002	39.0	36.0	41.0	31.0	41.0
37	36.91772338945245	39.0	36.0	41.0	31.0	41.0
38	36.80423501245023	39.0	35.0	40.0	31.0	41.0
39	36.67878632489157	39.0	35.0	40.0	30.0	41.0
40	36.540760312667395	39.0	35.0	40.0	30.0	41.0
41	36.40324048949493	39.0	35.0	40.0	30.0	41.0
42	36.265101893085486	39.0	35.0	40.0	30.0	41.0
43	34.560409771251805	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	12.0
11	11.0
12	7.0
13	4.0
14	5.0
15	9.0
16	18.0
17	34.0
18	53.0
19	152.0
20	304.0
21	642.0
22	1259.0
23	2206.0
24	3761.0
25	5965.0
26	9188.0
27	13680.0
28	19606.0
29	27164.0
30	35364.0
31	46231.0
32	59478.0
33	77590.0
34	103033.0
35	140870.0
36	207919.0
37	343234.0
38	631085.0
39	544962.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.54903437072037	17.175069287649954	12.305143602007869	25.970752739621805
2	16.80587232073827	20.714742459278387	36.751524064429816	25.727861155553523
3	18.73805442213968	22.153357251608945	30.776162409723756	28.33242591652762
4	13.325085955386756	14.793122161757088	35.183481437242676	36.69831044561348
5	14.364114846423737	37.270378836987774	34.59127103147344	13.774235285115052
6	35.4558384135481	36.061550125909584	14.54693221288614	13.935679247656182
7	29.823418741924502	30.870099839303666	20.822137217253175	18.484344201518653
8	28.05927733266956	36.140271099199865	18.73260112566594	17.067850442464643
9	26.689180571839703	13.427423220664123	18.22170640683175	41.66168980066442
10	14.851877033441902	27.978577340497672	34.20852877977214	22.961016846288285
11	37.42412661498935	20.839112801437558	21.492145054167946	20.24461552940514
12	21.130292446216863	25.038458933599077	31.448545069296447	22.382703550887612
13	31.945014939393644	18.785814744482277	24.608484097923615	24.660686218200464
14	21.64061544848526	18.993831618037042	27.496928122913783	31.868624810563915
15	25.09853315120496	28.079375368867133	22.463799346835813	24.3582921330921
16	24.346681888986716	26.099872727096812	24.468677408487967	25.084767975428502
17	23.937508740666726	26.385291228020797	26.234973749413992	23.44222628189849
18	23.840492837270993	25.503132566998588	26.806162576840904	23.850212018889515
19	24.876267341702675	25.607492829354918	26.698503949681907	22.817735879260496
20	24.302351866038894	25.616904163591858	26.454732801666246	23.626011168703005
21	23.752272573349035	25.903773945029013	26.7202731573795	23.623680324242454
22	24.31405006653901	25.432591538418915	26.26822126662486	23.985137128417218
23	23.505818755293877	25.487168481353685	26.552804181798834	24.454208581553612
24	23.697255848440577	25.322206263023045	26.801412931525064	24.17912495701131
25	24.060119954931146	25.26534245382509	26.631569133286483	24.042968457957283
26	23.748534426572682	25.639245087855244	26.732235227063832	23.879985258508242
27	24.070190962128617	25.904301683397442	26.583808810943886	23.44169854353006
28	23.87928160735034	26.125248146978652	26.409039454599988	23.58643079107102
29	23.567080384228714	26.13202078937346	26.597969790496663	23.702929035901164
30	23.70367666525643	26.202429883361024	26.578267558075407	23.515625893307135
31	23.50401564920175	25.525913273235663	26.733466616590157	24.236604460972426
32	23.201533607698646	26.13307626611031	26.778368356103776	23.887021770087262
33	23.652486043519065	25.53251000284099	26.680736757944885	24.13426719569506
34	23.710669198638083	25.6542856313554	27.019500812277304	23.615544357729213
35	23.662864898098118	25.669634022237137	26.784305412748576	23.883195666916173
36	23.4292087354773	25.365041027260325	27.089953884462236	24.115796352800135
37	23.351807108108083	25.16093821327139	27.00890206671141	24.47835261190912
38	23.13235590323741	25.22945624477209	27.40219908578123	24.235988766209264
39	22.948043278064468	25.004859590809257	27.844487816719983	24.20260931440629
40	23.059220161012973	24.964839431203586	27.962833145839618	24.01310726194382
41	22.7705472734837	24.69753994759558	28.13940560827564	24.392507170645082
42	22.31770377517642	24.843063802689176	28.225470940526527	24.61376148160788
43	22.238630976307185	24.427909619527025	28.2197097966712	25.11374960749459
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	179.0
1	263.0
2	347.0
3	1739.0
4	3131.0
5	3131.0
6	4164.5
7	5198.0
8	4967.0
9	4736.0
10	6054.5
11	7373.0
12	7373.0
13	11929.0
14	16485.0
15	26590.0
16	36695.0
17	28457.0
18	20219.0
19	20219.0
20	21548.5
21	22878.0
22	19098.5
23	15319.0
24	18483.5
25	21648.0
26	21648.0
27	26044.0
28	30440.0
29	34066.0
30	37692.0
31	42076.5
32	46461.0
33	46461.0
34	52718.5
35	58976.0
36	68025.5
37	77075.0
38	87343.0
39	97611.0
40	97611.0
41	109827.0
42	122043.0
43	135311.0
44	148579.0
45	161058.0
46	173537.0
47	173537.0
48	185250.5
49	196964.0
50	199393.5
51	201823.0
52	199913.5
53	198004.0
54	198004.0
55	187601.0
56	177198.0
57	164942.5
58	152687.0
59	139131.5
60	125576.0
61	125576.0
62	110331.5
63	95087.0
64	80734.0
65	66381.0
66	55250.0
67	44119.0
68	44119.0
69	35701.5
70	27284.0
71	22630.5
72	17977.0
73	14305.5
74	10634.0
75	10634.0
76	8315.5
77	5997.0
78	4809.0
79	3621.0
80	2737.0
81	1853.0
82	1853.0
83	1460.0
84	1067.0
85	821.0
86	575.0
87	408.0
88	241.0
89	241.0
90	160.5
91	80.0
92	58.5
93	37.0
94	30.0
95	23.0
96	23.0
97	13.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2273854.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.14858476943041
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.836894789401	52.10344362624907
2	9.118927501093053	11.334568776159434
3	2.7057037749324624	5.044669812520739
4	1.2677061059845691	3.151445615620261
5	0.7260051039695365	2.2560094873544942
6	0.4498166460302758	1.6773280773908077
7	0.3067239471830034	1.334372146262203
8	0.22854878106147256	1.1363186634999134
9	0.1759666047969595	0.9842467909331428
>10	1.0841895648194122	12.39748529140564
>50	0.06923777790518243	2.9150363334663765
>100	0.028508796610456397	2.987785870851794
>500	0.00106236372754386	0.4255492801662773
>1k	4.249454910174651E-4	0.4642835961101787
>5k	2.1247274550873255E-4	0.8605620579778255
>10k+	7.082424850291086E-5	0.9268945740318678
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	21058	0.9260928801937152	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7349	0.32319577246384334	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6851	0.3012946301741449	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5351	0.23532733412083626	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	2558	0.11249622886957562	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.397819736887241E-5	4.397819736887241E-5
2	0.0	0.0	0.0	4.397819736887241E-5	4.397819736887241E-5
3	0.0	0.0	0.0	4.397819736887241E-5	4.397819736887241E-5
4	0.0	0.0	0.0	4.397819736887241E-5	4.397819736887241E-5
5	0.0	0.0	0.0	4.397819736887241E-5	4.397819736887241E-5
6	0.0	0.0	0.0	4.397819736887241E-5	4.397819736887241E-5
7	0.0	0.0	0.0	4.397819736887241E-5	4.397819736887241E-5
8	4.397819736887241E-5	0.0	0.0	4.397819736887241E-5	4.397819736887241E-5
9	4.397819736887241E-5	0.0	0.0	4.397819736887241E-5	4.397819736887241E-5
10	4.397819736887241E-5	0.0	0.0	8.795639473774482E-5	4.397819736887241E-5
11	4.397819736887241E-5	0.0	0.0	1.7591278947548964E-4	4.397819736887241E-5
12	4.397819736887241E-5	0.0	0.0	3.958037763198517E-4	4.397819736887241E-5
13	4.397819736887241E-5	0.0	0.0	4.397819736887241E-4	4.397819736887241E-5
14	4.397819736887241E-5	0.0	0.0	4.837601710575965E-4	4.397819736887241E-5
15	8.795639473774482E-5	0.0	0.0	5.27738368426469E-4	4.397819736887241E-5
16	1.3193459210661724E-4	0.0	0.0	8.795639473774482E-4	4.397819736887241E-5
17	1.3193459210661724E-4	0.0	0.0	0.0012313895263284275	4.397819736887241E-5
18	1.3193459210661724E-4	0.0	0.0	0.0012313895263284275	4.397819736887241E-5
19	1.3193459210661724E-4	0.0	0.0	0.0015392369079105343	4.397819736887241E-5
20	1.3193459210661724E-4	0.0	0.0	0.002154931671074748	4.397819736887241E-5
21	1.3193459210661724E-4	0.0	0.0	0.0036501903816164097	4.397819736887241E-5
22	1.3193459210661724E-4	0.0	0.0	0.006376838618486499	4.397819736887241E-5
23	1.3193459210661724E-4	0.0	0.0	0.007520271750077182	4.397819736887241E-5
24	1.3193459210661724E-4	0.0	0.0	0.011082505736955846	4.397819736887241E-5
25	1.3193459210661724E-4	0.0	0.0	0.013545284789612701	4.397819736887241E-5
26	1.3193459210661724E-4	0.0	0.0	0.01622795482911392	4.397819736887241E-5
27	1.3193459210661724E-4	0.0	0.0	0.027134547776594277	4.397819736887241E-5
28	1.3193459210661724E-4	0.0	0.0	0.11447524775117487	4.397819736887241E-5
29	1.3193459210661724E-4	0.0	0.0	0.2759631884896744	4.397819736887241E-5
30	1.3193459210661724E-4	0.0	0.0	0.4636621348600218	4.397819736887241E-5
31	1.3193459210661724E-4	0.0	0.0	0.9047194762724432	4.397819736887241E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGATCG	120	0.0	23.124998	5
TATACCG	105	9.840733E-10	21.142859	5
ATACGAT	85	2.7250138E-5	17.411764	6
TACGTAG	65	0.0015805764	17.076923	12
ACGCGCA	185	1.8189894E-12	17.0	19
AATCGGG	175	7.2759576E-12	16.914286	25
TACCGAC	110	7.81587E-7	16.818182	7
ACCGATT	200	0.0	16.650002	20
GTCTTAT	270	0.0	16.444443	1
AGTCGGT	285	0.0	16.228071	11
CAATCGG	230	0.0	16.086956	24
CGACCGA	185	1.8189894E-11	16.0	18
AACGCAG	8265	0.0	15.892318	5
TCTTAGA	805	0.0	15.627328	2
CGACGCG	190	2.7284841E-11	15.578948	17
ATACCGA	95	7.064534E-5	15.578948	6
CGCGCAA	215	0.0	15.488372	20
CGCGAAC	85	5.367019E-4	15.235294	12
CTTATAC	2290	0.0	15.187774	37
CGCAATC	195	4.1836756E-11	15.179487	22
>>END_MODULE