FastQCFastQC Report
Thu 9 Feb 2017
SRR2727307.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727307.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8499037
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT705400.829976384383313No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229420.269936464566515No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT220550.2594999880574705No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT178920.21051796809450296No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA91040.1071180181942966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC68250.019.16410337
AACGCAG264100.016.1673625
GAGTACT192000.014.38567711
CGTTACG9400.014.36702124
TCTTATA112850.014.34426237
TCGTTAC11150.014.26905823
ACGCAGA301100.014.2359686
AGTACTT196300.014.0799812
CGCAGAG305750.014.00735957
AGAGTAC312300.013.70172810
TTAGTAC4900.013.5918363
CAGAGTA320350.013.43827159
GTACTTT206800.013.40981713
TATACAC22500.013.32000137
GTTACGG16300.013.2791425
GCAGAGT328350.013.1221258
TACTTTT211650.013.06756514
CATTACG7250.013.01379324
CTCGTTA12950.012.85714222
ACTTTTT221550.012.52538915
Produced by FastQC (version 0.11.5)