##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727307.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8499037
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.086138700184506	33.0	31.0	34.0	30.0	34.0
2	32.23139656881126	33.0	31.0	34.0	30.0	34.0
3	32.314573286361735	34.0	31.0	34.0	30.0	34.0
4	35.895982097736486	37.0	35.0	37.0	35.0	37.0
5	35.77792295762449	37.0	35.0	37.0	35.0	37.0
6	35.79707948088707	37.0	35.0	37.0	35.0	37.0
7	35.740133029189074	37.0	35.0	37.0	35.0	37.0
8	35.716078303930196	37.0	35.0	37.0	33.0	37.0
9	37.439109630891124	39.0	37.0	39.0	35.0	39.0
10	37.27392774028399	39.0	37.0	39.0	34.0	39.0
11	37.38999324276386	39.0	37.0	39.0	34.0	39.0
12	37.29068446225143	39.0	37.0	39.0	34.0	39.0
13	37.35609657894182	39.0	37.0	39.0	34.0	39.0
14	38.49616562441133	40.0	38.0	41.0	34.0	41.0
15	38.45962324908104	40.0	38.0	41.0	34.0	41.0
16	38.39600027626659	40.0	38.0	41.0	34.0	41.0
17	38.36252977837371	40.0	38.0	41.0	34.0	41.0
18	38.32111485101194	40.0	38.0	41.0	34.0	41.0
19	38.33407490754541	40.0	38.0	41.0	34.0	41.0
20	38.22068817914312	40.0	38.0	41.0	34.0	41.0
21	38.14622150721311	40.0	38.0	41.0	33.0	41.0
22	38.07275153643877	40.0	38.0	41.0	33.0	41.0
23	37.95493054095423	40.0	37.0	41.0	33.0	41.0
24	37.84693348199331	40.0	37.0	41.0	33.0	41.0
25	37.755420408218015	40.0	37.0	41.0	32.0	41.0
26	37.390145495307294	39.0	37.0	41.0	32.0	41.0
27	37.171195630752045	39.0	36.0	41.0	31.0	41.0
28	37.00947542645125	39.0	36.0	40.0	31.0	41.0
29	36.95421457748684	39.0	36.0	40.0	31.0	41.0
30	36.86307742865456	39.0	36.0	40.0	30.0	41.0
31	36.64255915111324	39.0	36.0	40.0	30.0	41.0
32	36.522862296046014	39.0	35.0	40.0	30.0	41.0
33	36.328833607854634	39.0	35.0	40.0	30.0	41.0
34	36.18728380638889	39.0	35.0	40.0	29.0	41.0
35	36.028876448002286	38.0	35.0	40.0	28.0	41.0
36	35.86854557757544	38.0	35.0	40.0	27.0	41.0
37	35.624046347839176	38.0	35.0	40.0	26.0	41.0
38	35.50591531722947	38.0	35.0	40.0	26.0	41.0
39	35.3326938098987	38.0	34.0	40.0	25.0	41.0
40	35.182738585559754	38.0	34.0	40.0	25.0	41.0
41	35.028141070570705	38.0	34.0	40.0	24.0	41.0
42	34.813566878223966	38.0	33.0	40.0	24.0	41.0
43	31.46010471539305	35.0	28.0	38.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	13.0
10	43.0
11	60.0
12	58.0
13	46.0
14	76.0
15	96.0
16	242.0
17	480.0
18	938.0
19	2034.0
20	3954.0
21	7725.0
22	13566.0
23	22143.0
24	33243.0
25	47284.0
26	64546.0
27	85733.0
28	112372.0
29	142117.0
30	178066.0
31	223048.0
32	278336.0
33	350270.0
34	447379.0
35	589301.0
36	823625.0
37	1291630.0
38	2228953.0
39	1551659.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.44835032486622	18.50613193000572	12.93352411573217	26.111993629395897
2	18.5131327231544	21.7466990672002	34.324171079617614	25.41599713002779
3	18.691364680492626	22.32272903388937	30.858743172903004	28.127163112715003
4	14.797029357561334	15.610168540271092	33.1963609524232	36.396441149744376
5	14.518656643099684	37.48774125821549	33.71379604536372	14.279806053321101
6	36.33271628303301	35.390927230932164	13.87444248095402	14.40191400508081
7	30.047627748884963	30.987393042294087	20.025809982942775	18.93916922587818
8	28.56189471818984	35.15526523769693	18.402343700821636	17.8804963432916
9	27.240462654769004	13.624896561810473	17.910993916134263	41.223646867286256
10	15.571564166622643	28.215949642294767	33.28547693109231	22.92700925999028
11	37.44252437070224	21.294247807133914	20.61160576192338	20.65162206024047
12	21.62909750834124	25.467544146472125	29.728932819094684	23.17442552609196
13	32.237016970275576	19.47061767115498	23.41158180626817	24.880783552301278
14	22.418986998174027	20.157989664005463	25.894827849319867	31.52819548850064
15	25.777285120655435	28.23756385576389	21.534427959308804	24.45072306427187
16	24.73021355242953	26.16935306905947	23.824346217106715	25.276087161404288
17	24.405941520198112	26.28696639395734	25.21455077792931	24.092541307915237
18	24.235981088210345	25.58888730570299	25.870578043135943	24.30455356295072
19	25.398430433942103	25.568414398007683	25.81157135802562	23.221583810024597
20	24.782278274585696	25.443847344116747	25.62179691652125	24.152077464776305
21	24.520272120241387	25.78756863865871	25.678579820278465	24.01357942082144
22	25.186453477023342	25.236682697110275	25.004656409896793	24.572207415969597
23	24.524707916908703	25.369109464989975	25.394100531624936	24.712082086476386
24	24.412177520817945	25.20213760688417	25.485605016191837	24.90007985610605
25	24.682502264668337	24.9474852268557	25.47106219210482	24.89895031637114
26	24.29058727477007	25.2913006497089	25.885509146506834	24.532602929014192
27	24.854557051581256	25.596935276314248	25.445165140474153	24.103342531630346
28	24.45853571410502	25.833444424350667	25.389935353852444	24.31808450769187
29	24.31240151090059	25.977201887696218	25.594323215677257	24.116073385725937
30	24.614847540962582	25.797734496272927	25.305655217173427	24.28176274559106
31	24.363430821633088	25.25710854064996	25.5708852661778	24.808575371539153
32	23.910438323777154	25.707124230662835	25.563543257900868	24.818894187659144
33	24.217178958039597	25.180723416076432	25.63477485743385	24.96732276845012
34	24.393987224670276	25.02561172518722	26.179518926673694	24.40088212346881
35	24.206283606013244	25.033612631642853	26.0508572912437	24.709246471100197
36	23.92582830266535	24.574348835050372	26.58828288428442	24.911539977999862
37	23.607439289886607	24.336039483061434	26.850877340573998	25.20564388647796
38	23.43839660893346	24.18931697791173	27.247004572400378	25.125281840754425
39	23.033515444161498	24.09668295360992	27.92894065527659	24.94086094695199
40	23.302487093537774	23.937935556698953	28.13183423016043	24.627743119602844
41	22.72508050029668	23.91683904894166	28.51238322647613	24.84569722428553
42	22.313222074453847	24.122474110890447	28.52631421654006	25.037989598115644
43	22.332353653713945	23.310558596226844	28.421055232492808	25.936032517566403
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1871.0
1	1809.0
2	1747.0
3	4034.5
4	6322.0
5	6322.0
6	7064.5
7	7807.0
8	8051.5
9	8296.0
10	12452.5
11	16609.0
12	16609.0
13	32939.0
14	49269.0
15	79890.5
16	110512.0
17	81698.0
18	52884.0
19	52884.0
20	61958.0
21	71032.0
22	60319.0
23	49606.0
24	60290.5
25	70975.0
26	70975.0
27	86868.5
28	102762.0
29	118563.0
30	134364.0
31	155308.0
32	176252.0
33	176252.0
34	201471.5
35	226691.0
36	259300.0
37	291909.0
38	331471.5
39	371034.0
40	371034.0
41	413887.5
42	456741.0
43	500252.0
44	543763.0
45	588035.5
46	632308.0
47	632308.0
48	674065.0
49	715822.0
50	738117.0
51	760412.0
52	751587.5
53	742763.0
54	742763.0
55	704497.5
56	666232.0
57	622569.5
58	578907.0
59	533391.0
60	487875.0
61	487875.0
62	430182.5
63	372490.0
64	316568.5
65	260647.0
66	220314.0
67	179981.0
68	179981.0
69	149041.0
70	118101.0
71	99629.5
72	81158.0
73	67666.0
74	54174.0
75	54174.0
76	45104.5
77	36035.0
78	30363.5
79	24692.0
80	19828.0
81	14964.0
82	14964.0
83	12035.0
84	9106.0
85	7419.0
86	5732.0
87	4746.0
88	3760.0
89	3760.0
90	2828.0
91	1896.0
92	1396.0
93	896.0
94	656.0
95	416.0
96	416.0
97	288.0
98	160.0
99	112.0
100	64.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	8499037.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.16381205403036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.52520509045054	45.787662963663436
2	9.681902397019048	10.875450931032876
3	3.2908883116777603	5.54486497883625
4	1.561894278639514	3.5088774685509994
5	0.8680510238149283	2.4376527277425133
6	0.5774884653613142	1.946037217915396
7	0.4057494277830198	1.5951904222125124
8	0.3112244483853563	1.3983641140587537
9	0.2379438532383988	1.2027450467423892
>10	1.3877618077050085	14.525605145441226
>50	0.1082300484165993	4.161263917951585
>100	0.04095714448336789	3.8849849962637095
>500	0.0018446053938306834	0.7130475359355501
>1k	7.333760476181816E-4	0.678703734249382
>5k	4.1907194621036176E-5	0.16931109336618694
>10k+	8.381438924207235E-5	1.5702377060371835
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	70540	0.829976384383313	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22942	0.269936464566515	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	22055	0.2594999880574705	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	17892	0.21051796809450296	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	9104	0.1071180181942966	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1766038905348924E-5
2	1.1766038905348924E-5	0.0	0.0	0.0	1.1766038905348924E-5
3	1.1766038905348924E-5	0.0	0.0	0.0	1.1766038905348924E-5
4	1.1766038905348924E-5	0.0	0.0	1.1766038905348924E-5	1.1766038905348924E-5
5	2.3532077810697848E-5	2.3532077810697848E-5	0.0	1.1766038905348924E-5	1.1766038905348924E-5
6	2.3532077810697848E-5	2.3532077810697848E-5	0.0	1.1766038905348924E-5	1.1766038905348924E-5
7	2.3532077810697848E-5	2.3532077810697848E-5	0.0	1.1766038905348924E-5	2.3532077810697848E-5
8	2.3532077810697848E-5	2.3532077810697848E-5	0.0	1.1766038905348924E-5	5.883019452674462E-5
9	2.3532077810697848E-5	2.3532077810697848E-5	0.0	2.3532077810697848E-5	9.412831124279139E-5
10	4.7064155621395695E-5	2.3532077810697848E-5	0.0	3.529811671604677E-5	9.412831124279139E-5
11	5.883019452674462E-5	2.3532077810697848E-5	0.0	7.059623343209355E-5	9.412831124279139E-5
12	5.883019452674462E-5	2.3532077810697848E-5	0.0	7.059623343209355E-5	9.412831124279139E-5
13	5.883019452674462E-5	2.3532077810697848E-5	0.0	1.1766038905348924E-4	1.2942642795883816E-4
14	5.883019452674462E-5	2.3532077810697848E-5	0.0	1.8825662248558278E-4	1.52958505769536E-4
15	5.883019452674462E-5	4.7064155621395695E-5	0.0	3.294490893497699E-4	1.6472454467488494E-4
16	7.059623343209355E-5	4.7064155621395695E-5	0.0	6.706642176048886E-4	2.1178870029628063E-4
17	7.059623343209355E-5	4.7064155621395695E-5	0.0	0.0010236453847653564	2.1178870029628063E-4
18	7.059623343209355E-5	4.7064155621395695E-5	0.0	0.0011413057738188456	2.2355473920162956E-4
19	7.059623343209355E-5	4.7064155621395695E-5	0.0	0.001541351096600709	2.2355473920162956E-4
20	8.236227233744247E-5	4.7064155621395695E-5	0.0	0.0022120153142055976	2.2355473920162956E-4
21	9.412831124279139E-5	4.7064155621395695E-5	0.0	0.0035180456326993283	2.588528559176763E-4
22	9.412831124279139E-5	4.7064155621395695E-5	0.0	0.005706528869094228	2.823849337283742E-4
23	1.0589435014814031E-4	4.7064155621395695E-5	0.0	0.00710668749883075	4.3534343949791016E-4
24	1.0589435014814031E-4	4.7064155621395695E-5	0.0	0.009212808462888208	4.3534343949791016E-4
25	1.0589435014814031E-4	8.236227233744247E-5	0.0	0.010177623653126818	4.471094784032591E-4
26	1.0589435014814031E-4	8.236227233744247E-5	0.0	0.01168367663301148	4.5887551730860803E-4
27	1.0589435014814031E-4	8.236227233744247E-5	0.0	0.018519745237019207	4.7064155621395694E-4
28	1.0589435014814031E-4	8.236227233744247E-5	0.0	0.05694762830188879	4.8240759511930585E-4
29	1.0589435014814031E-4	8.236227233744247E-5	0.0	0.14329858782824453	4.8240759511930585E-4
30	1.0589435014814031E-4	8.236227233744247E-5	0.0	0.26458291686458124	4.8240759511930585E-4
31	1.0589435014814031E-4	8.236227233744247E-5	0.0	0.6805947544410031	4.941736340246548E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	6825	0.0	19.164103	37
AACGCAG	26410	0.0	16.167362	5
GAGTACT	19200	0.0	14.385677	11
CGTTACG	940	0.0	14.367021	24
TCTTATA	11285	0.0	14.344262	37
TCGTTAC	1115	0.0	14.269058	23
ACGCAGA	30110	0.0	14.235968	6
AGTACTT	19630	0.0	14.07998	12
CGCAGAG	30575	0.0	14.0073595	7
AGAGTAC	31230	0.0	13.701728	10
TTAGTAC	490	0.0	13.591836	3
CAGAGTA	32035	0.0	13.4382715	9
GTACTTT	20680	0.0	13.409817	13
TATACAC	2250	0.0	13.320001	37
GTTACGG	1630	0.0	13.27914	25
GCAGAGT	32835	0.0	13.122125	8
TACTTTT	21165	0.0	13.067565	14
CATTACG	725	0.0	13.013793	24
CTCGTTA	1295	0.0	12.857142	22
ACTTTTT	22155	0.0	12.525389	15
>>END_MODULE